Agreement between PDL1 immunohistochemistry assays and polymerase chain reaction in non-small cell lung cancer: CLOVER comparison study.

The goal of the CLOVER study was to perform a pairwise comparison of four tests based on the same patient population with non-small cell lung cancer (NSCLC): three validated PDL1 immunohistochemistry (IHC) assays (Ventana SP142, Ventana SP263, Dako 22C3) and one PCR test. Four hundred seventy-three NSCLC samples were obtained from a biobank and were stained using PDL1 IHC assays. Four trained pathologists independently evaluated the percentage of tumor cells (TC) and immune cells (IC) that stained positive at any intensity.

RUSSCO-RSP comparative study of immunohistochemistry diagnostic assays for PD-L1 expression in urothelial bladder cancer.

In this collaborative study by the Russian Society of Clinical Oncology and the Russian Society of Pathology, we assessed the concordance among three validated, commercially available PD-L1 immunohistochemistry assays for patients with urothelial cancer. Tumors from 100 urothelial cancer patients were stained with the antibody clones 22C3 (Agilent), SP142 (Ventana Medical Systems), and SP263 (Ventana Medical Systems), which are used in clinical trials of second-line therapy with checkpoint inhibitors. Four trained pathologists independently evaluated the percentages of tumor cells (TC) and tumor-infiltrating immune cells (IC) that were stained at any intensity by each of the antibodies.

Laminar distribution of gap-junctions in rat somatic cortical columns.

Results about the spindle-like activity peculiarities and the dendro-dendritic gap-junctions numbers in the infragranular and supragranular layers (modules) of the rat somatic cortex columns are presented. During simultaneous recording of extracellular field potentials from the cortical columns, the following was revealed: (i) the spindle-like activity was more synchronized inside one cortical column than in different columns; (ii) in most of the experiments (9 out of 10) an amplitude of the spindle waves was two times more in the supragranular layers than in the infragranular layers. During electron microscopy investigation of the experimental objects used in the electrophysiological study, it was shown that the number of the gap-junctions was more in the supragranular columnar layers as compared with the infragranular layers (4.