The Restriction-Modification Systems of P7.

P7 (DSM 15243) is a bacterium that converts syngas (a mixture of CO, H, and CO) into hexanol. An optimized and scaled-up industrial process could therefore provide a renewable source of fuels and chemicals while consuming industry waste gases. However, the genetic engineering of this bacterium is hindered by its multiple restriction-modification (RM) systems: the genome of encodes at least ten restriction enzymes and eight methyltransferases (MTases).

Impact of different trace elements on metabolic routes during heterotrophic growth of C. ljungdahlii investigated through online measurement of the carbon dioxide transfer rate.

Synthesis gas fermentation using acetogenic clostridia is a rapidly increasing research area. It offers the possibility to produce platform chemicals from sustainable C1 carbon sources. The Wood-Ljungdahl pathway (WLP), which allows acetogens to grow autotrophically, is also active during heterotrophic growth.

Hexanol biosynthesis from syngas by P7 - product toxicity, temperature dependence and extraction.

converts syngas into industrial alcohols like hexanol, but titers may be limited by product toxicity. Investigation of IC at 30 °C (17.5 mM) and 37 °C (11.

Regulation of lactate metabolism in the acetogenic bacterium Acetobacterium woodii.

Acetogenic bacteria compete in an energy-limited environment by coupling different metabolic routes to their central metabolism of CO fixation. The underlying regulatory mechanisms are often still not understood. In this work, we analysed how lactate metabolism is regulated in the model acetogen Acetobacterium woodii.

Efficient whole cell biocatalyst for formate-based hydrogen production.

Background: Molecular hydrogen (H) is an attractive future energy carrier to replace fossil fuels. Biologically and sustainably produced H could contribute significantly to the future energy mix. However, biological H production methods are faced with multiple barriers including substrate cost, low production rates, and low yields.