Introduction: Plant growth is a plastic phenomenon controlled both by endogenous genetic programs and by environmental cues. The embryonic stem, the hypocotyl, is an ideal model system for the quantitative study of growth due to its relatively simple geometry and cellular organization, and to its essentially unidirectional growth pattern. The hypocotyl of Arabidopsis thaliana has been studied particularly well at the molecular-genetic level and at the cellular level, and it is the model of choice for analysis of the shade avoidance syndrome (SAS), a growth reaction that allows plants to compete with neighboring plants for light.
View Article and Find Full Text PDFProlonged consumption of water contaminated with fluoride ions (F) at concentrations exceeding 1.5 ppm can lead to considerable health implications, particularly in children and developing embryos. With irreversible and potentially severe forms of fluoride (F) toxicity such as skeletal fluorosis being endemic in at least 25 countries, constructing affordable, remote-access, reliable water-sampling methods for F contamination is an important goal.
View Article and Find Full Text PDFPlants are highly plastic in their potential to adapt to changing environmental conditions. For example, they can selectively promote the relative growth of the root and the shoot in response to limiting supply of mineral nutrients and light, respectively, a phenomenon that is referred to as balanced growth or functional equilibrium. To gain insight into the regulatory network that controls this phenomenon, we took a systems biology approach that combines experimental work with mathematical modeling.
View Article and Find Full Text PDFBackground: Genes involved in arbuscular mycorrhizal (AM) symbiosis have been identified primarily by mutant screens, followed by identification of the mutated genes (forward genetics). In addition, a number of AM-related genes has been identified by their AM-related expression patterns, and their function has subsequently been elucidated by knock-down or knock-out approaches (reverse genetics). However, genes that are members of functionally redundant gene families, or genes that have a vital function and therefore result in lethal mutant phenotypes, are difficult to identify.
View Article and Find Full Text PDFMost terrestrial plants form arbuscular mycorrhiza (AM), mutualistic associations with soil fungi of the order Glomeromycota. The obligate biotrophic fungi trade mineral nutrients, mainly phosphate (P(i) ), for carbohydrates from the plants. Under conditions of high exogenous phosphate supply, when the plant can meet its own P requirements without the fungus, AM are suppressed, an effect which could be interpreted as an active strategy of the plant to limit carbohydrate consumption of the fungus by inhibiting its proliferation in the roots.
View Article and Find Full Text PDFPlant excitability, as measured by the appearance and circulation of action potentials (APs) after biotic and abiotic stress treatments, is a far lesser and more versatile phenomenon than in animals. To examine the genetic basis of plant excitability we used different Arabidopsis thaliana accessions. APs were induced by wounding (W) with a subsequent deposition (D) of 5μL of 1M KCl onto adult leaves.
View Article and Find Full Text PDFA platform to study ultrasound as a source for wireless energy transfer and communication for implanted medical devices is described. A tank is used as a container for a pair of electroacoustic transducers, where a control unit is fixed to one wall of the tank and a transponder can be manually moved in three axes and rotate using a mechanical system. The tank is filled with water to allow acoustic energy and data transfer, and the system is optimized to avoid parasitic effects due to cables, reflection paths and cross talk problems.
View Article and Find Full Text PDFWe present a new superheterodyne technique for long-distance measurements by two-wavelength interferometry (TWI). While conventional systems use two acousto-optic modulators to generate two different heterodyne frequencies, here the two frequencies result from synchronized sweeps of optical and radio frequencies. A distributed feedback laser source is injected in an intensity modulator that is driven at the half-wave voltage mode.
View Article and Find Full Text PDFWe present a new technique applied to the variable optical synthetic wavelength generation in optical interferometry. It consists of a chain of optical injection locking among three lasers: first a distributed-feedback laser is used as a master to injection lock an intensity-modulated laser that is directly modulated around 15 GHz by a radio frequency generator on a sideband. A second distributed-feedback laser is injection locked on another sideband of the intensity-modulated laser.
View Article and Find Full Text PDFVoltage-elicited action potentials (APs) have been reproducibly obtained in Arabidopsis thaliana ecotype col. Excitations pulses (voltage-duration: V-t) were given in the 0- to 18-V and 0- to 35-s ranges, respectively, by two galvanically isolated Pt/Ir small wires inserted trough the main vein in the distal part of the leaf. Conventional liquid junction Ag/AgCl electrodes were placed at the zone between leaf/petiole (e1) and a second one on the petiole, near the central axis of the rosette (e2).
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