Publications by authors named "Patricia Stecum"
Article Synopsis
- Spinal nociceptive circuits become sensitized in neuropathic pain, largely influenced by gene expression changes through transcriptional and translational controls.
- The study indicates that during chronic neuropathic pain, it's primarily translational regulation that impacts gene expression in the spinal cord.
- Findings show that regulating translation in specific spinal neurons, particularly inhibitory ones, significantly affects pain sensitivity, with increased translation in these neurons linked to heightened hypersensitivity.
The fluorescent non-canonical amino acid tagging (FUNCAT) technique has been used to visualize newly synthesized proteins in cell lines and tissues. Here, we present a protocol for measuring protein synthesis in specific cell types in the mouse brain using in vivo FUNCAT. We describe steps for metabolically labeling newly synthesized proteins with azidohomoalanine, which introduces an azide group into the polypeptide.
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