Successful treatment of experimental murine vulvovaginal candidiasis with gentian violet.

This study evaluated the antifungal efficacy of gentian violet (GV) in an experimental vulvovaginal candidiasis (VVC) model. susceptibility and cytotoxicity assays were performed to validate the antifungal potential and safety of GV. The antifungal efficacy was then evaluated through comparative analysis of the fungal burden following treatment with GV or nystatin, as well as assessment of the vaginal tissue by histology and electron microscopy.

In vitro antifungal activity of curcumin mediated by photodynamic therapy on Sporothrix brasiliensis.

Background: Sporothrix brasiliensis is a pathogenic dimorphic fungus that affects humans and animals causing sporotrichosis. The treatment of this disease with conventional antifungals commonly results in therapeutic failures and resistance. Therefore, this study aimed to evaluate the in vitro effect of curcumin (CUR) mediated by photodynamic therapy (PDT) in its pure state and incorporated into pharmaceutical formulation in gel form, on the filamentous and yeast forms of S.

Analysis of the antifungal potential of extract against .

To investigate the antifungal potential of extracts against . Extracts were obtained as aqueous (EfraMat-22 and EfraMat-45) and methanolic/ethyl acetate fractions. Broth microdilution and disk diffusion assays showed that EfraMat-45 provided the best results in terms of minimum inhibitory concentration.

Cell wall associated proteins involved in filamentation with impact on the virulence of Candida albicans.

Candida albicans is a commensal microorganism of the human microbiota that can be associated with superficial to disseminated infections. This species possesses several attributes that contribute to pathogenesis and virulence, such as the ability to transition from yeast to hyphae forms. During this transition, several changes occur in the fungal cell wall, which is the first point of contact between the pathogen and the host.

The zoonosis sporotrichosis can be successfully treated by photodynamic therapy: A scoping review.

Sporotrichosis is a worldwide zoonosis, prevalent in tropical and subtropical regions. In recent years, there has been a substantial increase in human and feline cases reported in Brazil. Despite this, the antifungal treatment for sporotrichosis is still limited, and thus, research into new therapeutic modalities must be encouraged.

Hypericin-P123-photodynamic therapy in an ex vivo model as an alternative treatment approach for onychomycosis caused by Fusarium spp.

BackgroundFusarium has been considered an opportunistic pathogen, causing several infections in humans, including onychomycosis. In addition, a high resistance to conventional antifungals has been linked to this genus. Photodynamic Therapy (PDT), known as a non-invasive therapy, can be an alternative treatment for fungal infections, based on the excitation of a photosensitizing compound (PS) by a specific length of light, causing damage to the target.

A systematic review of photodynamic therapy as an antiviral treatment: Potential guidance for dealing with SARS-CoV-2.

Background: SARS-CoV-2, which causes the coronavirus disease (COVID-19), presents high rates of morbidity and mortality around the world. The search to eliminate SARS-CoV-2 is ongoing and urgent. This systematic review seeks to assess whether photodynamic therapy (PDT) could be effective in SARS-CoV-2 inactivation.

Impact of serial systemic infection on virulence factors.

To evaluate changes in virulence and pathogenicity approaches from after successive passages in a murine model of systemic candidiasis. Phenotypic assays were performed using colonies recovered from animals infected serially, totalizing five passages. A progressive infection was observed along the passages, with increased fungal burden and the presence of greater inflammatory areas in the histopathological findings.

Vulvovaginal candidiasis in a murine model of diabetes emphasizing the invasive ability of etiological agents.

To compare the pathogenesis of vulvovaginal candidiasis by three species in diabetic mice. Estrogenized and diabetic mice were challenged with , and . Diabetic animals infected with and maintained the highest fungal burden, despite of high levels of proinflammatory cytokines (IL-6 and TNF-α), respectively.

Copolymeric micelles as efficient inert nanocarrier for hypericin in the photodynamic inactivation of species.

To evaluate the efficacy of photodynamic inactivation (PDI) mediated by hypericin encapsulated in P-123 copolymeric micelles (P123-Hyp) alone and in combination with fluconazole (FLU) against planktonic cells and biofilm formation of species PDI was performed using P123-Hyp and an LED device with irradiance of 3.0 mW/cm . Most of isolates (70%) were completely inhibited with concentrations up to 2.

Artepillin C Induces Selective Oxidative Stress and Inhibits Migration and Invasion in a Comprehensive Panel of Human Cervical Cancer Cell Lines.

Background: Artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) is the main bioactive component of Brazilian green propolis, and possesses, among other things, anticancer properties. However, to the best of our knowledge, there are no studies of artepillin C in cervical cancer.

Method: To explore a new therapeutic candidate for cervical cancer, we have evaluated the effects of artepillin C on cellular viability in a comprehensive panel of human cervical cancer-derived cell lines including HeLa (human papillomavirus/HPV 18-positive), SiHa (HPV 16-positive), CaSki (HPV 16- and 18-positive) and C33A (HPV-negative) cells compared to a spontaneously immortalized human epithelial cell line (HaCaT).

Candida parapsilosis isolates from burn wounds can penetrate an acellular dermal matrix.

We isolated and identified yeasts from burn wounds and evaluated the ability of Candida parapsilosis isolates from burn wounds to penetrate an acellular dermal matrix (ADM). A prospective study was conducted with patients from the burn treatment center of North Paraná University Hospital in Londrina, Brazil from February 2015 to January 2016. Yeast cultures were obtained from the tissue of burn wounds that had been debrided and cleansed with 2% chlorhexidine.

γ-irradiation from radiotherapy improves the virulence potential of Candida tropicalis.

Aim: To evaluate if radiation used in radiotherapy can cause changes in the virulence potential of Candida tropicalis ATCC 750.

Materials & Methods: C. tropicalis was exposed in vitro to identical dose and scheme of irradiation would be used in patients with head and neck cancer.

Propolis: a potential natural product to fight Candida species infections.

Aim: To evaluate the effect of propolis against Candida species planktonic cells and its counterpart's biofilms.

Materials & Methods: The MIC values, time-kill curves and filamentation form inhibition were determined in Candida planktonic cells. The effect of propolis on Candida biofilms was assessed through quantification of CFUs.

Cellular Structural Changes in Candida albicans Caused by the Hydroalcoholic Extract from Sapindus saponaria L.

Vulvovaginal candidiasis (VVC) is a disease caused by the abnormal growth of yeast-like fungi in the mucosa of the female genital tract. Candida albicans is the principal etiological agent involved in VVC, but reports have shown an increase in the prevalence of Candida non-C. albicans (CNCA) cases, which complicates VVC treatment because CNCA does not respond well to antifungal therapy.