Comparison of CellRox green fluorescence upon thawing on and embryos cryopreserved by slow freezing or vitrification.

The aim of this study was to compare the levels of reactive oxygen species (ROS) in and embryos cryopreserved using either slow freezing or vitrification. Embryos were divided into four groups based on subspecies and freezing method: slow freezing (BiSF; = 8), vitrification (BiVT; = 10), slow freezing (BtSF; = 9), and vitrification (BtVT; = 6). After thawing, the embryos were incubated with CellRox Green and images were obtained using a confocal microscope.