Publications by authors named "Patricia Estima Antonia Abreu"
High level SARS-CoV-2 nucleocapsid refolding using mild condition for inclusion bodies solubilization: Application of high pressure at pH 9.0.
Rosa Maria Chura-Chambi Alvaro Rossan de Brandão Prieto-da-Silva Matheus Martins Di Lela João Ezequiel Oliveira Patricia Estima Antonia Abreu
PLoS One
February 2022
Article Synopsis
- - The SARS-CoV-2 Nucleocapsid (N) protein is crucial for diagnosis, and a specific 35 kDa fragment (N122-419) was engineered to minimize non-specific antibody binding while being expressed in E. coli.
- - N122-419 was successfully extracted and solubilized under specific conditions that maintain its structure, yielding high amounts of pure protein quickly without the need for dialysis.
- - Antibody binding tests confirmed that this fragment can effectively differentiate between COVID-19-positive and negative patient sera, suggesting its potential for broader applications in protein refolding.