Unfolding Case Studies for Nursing Leadership.

The education of nursing students has changed radically during the COVID-19 pandemic, with more content being delivered virtually. With less face-to-face (F2F) contact with educators, content translation to real-world scenarios is diminished. To determine if an educational seminar using unfolding case studies will improve students' understanding of concepts.

Advancing Nursing Science and Practice in Unison.

Purpose: The purpose of this article is to explore the strengths of collaborative Doctor of Nursing Practice (DNP) and the Doctor of Philosophy (PhD) research drawing on the assets of the complementary skill sets, explore examples of current DNP/PhD collaborations, and provide a model for building these partnerships.

Design: A DNP and PhD collaboration was examined through case analysis. Courtney & Neiheisel's model was used as a framework to examine the development, implementation, and evaluation of DNP and PhD collaboration.

Hospice Staff Comfort with Telehospice.

Telehospice has been recognized as a way to meet the needs of hospice patients and their caregivers. The purpose of this study was to examine the comfort of hospice staff using telehospice to connect virtually with patients and caregivers. The hospice in this study uses Alpha Virtual Assist (AVA), which employs a commercial remote patient monitoring platform and a communication platform that focuses on holistic patient and caregiver comfort as well as interpersonal and interprofessional communication.

Systematic Review of Telehospice Telemedicine and e-Health.

A 2012 systematic review by Oliver et al. of evidence-based research on telehospice included 26 research articles published between 2000 and 2010 on the use of telehospice connecting hospice professionals with caregivers and their care recipients. The present study replicated research by Oliver et al.

Caregiver Comfort With Telehospice.

The purpose of this mixed methods study was to examine comfort and emotional support that caregivers of home hospice patients derived from using electronic tablets for telehospice. The use of technology was intended to increase caregiver confidence in symptom management for individuals receiving home-based hospice care. Caregiver survey data were collected over a 2-year period.

The future of graduate and postdoctoral training in the biosciences.

This article summarizes the outcomes of the second national conference on the Future of Bioscience Graduate and Postdoctoral Training. Five topics were addressed during the conference: diversity in leadership positions; mentoring; modernizing the curriculum; experiential learning; and the need for better data on trainees. The goal of the conference was to develop a consensus around these five topics and to recommend policies that can be implemented by academic and research institutions and federal funding agencies in the United States.

The three-dimensional structure of MAP kinase p38beta: different features of the ATP-binding site in p38beta compared with p38alpha.

The p38 mitogen-activated protein kinases are activated in response to environmental stress and cytokines and play a significant role in transcriptional regulation and inflammatory responses. Of the four p38 isoforms known to date, two (p38alpha and p38beta) have been identified as targets for cytokine-suppressive anti-inflammatory drugs. Recently, it was reported that specific inhibition of the p38alpha isoform is necessary and sufficient for anti-inflammatory efficacy in vivo, while further inhibition of p38beta may not provide any additional benefit.

Myosin16b: The COOH-tail region directs localization to the nucleus and overexpression delays S-phase progression.

Rat Myo16a and Myo16b comprise the founding members of class XVI myosin and are characterized by an N-terminal ankyrin repeat domain thought to mediate an association with protein phosphatase 1 catalytic subunits 1alpha and 1gamma. Myo16b is the principal isoform and reveals predominant expression in developing neural tissue. Here, we use COS-7 cells as a model system to develop an understanding of Myo16b function.

Lattice stabilization and enhanced diffraction in human p38 alpha crystals by protein engineering.

Mitogen-activated protein (MAP) kinase p38 alpha is activated in response to environmental stress and cytokines, and plays a significant role in inflammatory responses. For these reasons, it is an important target for the treatment of a wide range of inflammatory and autoimmune diseases. The crystals of p38 alpha that we obtained by published procedures were usually small, quite mosaic, and difficult to reproduce and thus posed a difficulty for the intensive high-resolution studies required for a structure-guided drug discovery approach.

Structural basis for p38alpha MAP kinase quinazolinone and pyridol-pyrimidine inhibitor specificity.

The quinazolinone and pyridol-pyrimidine classes of p38 MAP kinase inhibitors have a previously unseen degree of specificity for p38 over other MAP kinases. Comparison of the crystal structures of p38 bound to four different compounds shows that binding of the more specific molecules is characterized by a peptide flip between Met109 and Gly110. Gly110 is a residue specific to the alpha, beta and gamma isoforms of p38.

Design and synthesis of potent, orally bioavailable dihydroquinazolinone inhibitors of p38 MAP kinase.

The development of potent, orally bioavailable (in rat) and selective dihydroquinazolinone inhibitors of p38alpha MAP kinase is described. These analogues are hybrids of a pyridinylimidazole p38alpha inhibitor reported by Merck Research Laboratories and VX-745. Optimization of the C-5 phenyl and the C-7 piperidinyl substituents led to the identification of 15i which gave excellent suppression of TNF-alpha production in LPS-stimulated whole blood (IC(50)=10nM) and good oral exposure in rats (F=68%, AUCn PO=0.

Photochemical preparation of a pyridone containing tetracycle: a Jak protein kinase inhibitor.

Jak3 is a protein tyrosine kinase that is associated with the shared gamma chain of receptors for cytokines IL2, IL4, IL7, IL9, and IL13. We have discovered that a pyridone-containing tetracycle (6) may be prepared from trisubstituted imidazole (5) in high yield by irradiation with >350 nm light. Compound 6 inhibits Jak3 with K(I)=5 nM; it also inhibits Jak family members Tyk2 and Jak2 with IC(50)=1 nM and murine Jak1with IC(50)=15 nM.

Caveolin-1 expression is maintained in rat and human astroglioma cell lines.

Caveolin-1 is the principal structural and functional component of caveolae, a plasmalemmal compartment that has been proposed to sequester lipid and protein components that participate in transmembrane signal transduction processes. Multiple studies reveal a reduction in the expression level of caveolin-1 mRNA and protein in many carcinomas as well as transformed cells. The human caveolin-1 gene is localized to a suspected tumor suppressor locus (7q31.