A feasibility study on the effects of Triton X-100 for the in vitro inactivation of Ebola virus on haematological assays.

Aims: The aim of this study was to check the effect of Triton X-100 on various, commonly used haematology test parameters.

Methods: Anonymised blood samples were treated with 10 µL of 10% Triton X-100 per 1 mL of blood. Treated and untreated samples were tested in parallel for blood film morphology, complete blood counts (CBCs), flow cytometry, blood grouping and antibody screens.

Flow cytometry and thromboelastography to assess platelet counts and coagulation in patients with haematological malignancies.

Background: Accurate platelet counts (PC) are necessary in order to follow recommendations for prophylactic platelet transfusion. We carried out a study comparing the standard way of counting platelets using a routine analyser and compared it with PC determined by flow cytometry (FC) and haemostatic data obtained with thromboelastography (TEG).

Materials And Methods: The study was carried out on 24 patients with haematological malignancies, all given one adult dose of platelets.

Evaluation of a method allowing preservation of fresh lymph nodes for flow cytometric immunophenotyping.

Background: Flow cytometric immunophenotyping (FCI) of lymph nodes (LN) requires fresh unfixed tissue, with analysis being carried out within few hours post surgery. This study evaluated a novel method for fresh LN preservation, in order to allow histomorphology-based FCI.

Methods: This study was carried out prospectively on 30 LN with suspected involvement by haematolymphoid neoplasms (HLN).