Publications by authors named "Patricia Ann Holden"

Copper formulations have been used for decades for antimicrobial and antifouling applications. With the development of nanoformulations of copper that are more effective than their ionic and microsized analogues, a key regulatory question is whether these materials should be treated as new or existing materials. To address this issue, here we compare the magnitude and mechanisms of toxicity of a series of Cu species (at concentration ranging from 2 to 250 μg/mL), including nano Cu, nano CuO, nano Cu(OH)2 (CuPro and Kocide), micro Cu, micro CuO, ionic Cu(2+) (CuCl2 and CuSO4) in two species of bacteria (Escherichia coli and Lactobacillus brevis).

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The aim of this study was to determine nutrient elements in soybean (Glycine max) plants cultivated in farm soil amended with nCeO2 at 0-1000 mg kg(-1) and nZnO at 0-500 mg kg(-1). Digested samples were analyzed by ICP-OES/MS. Compared to control, pods from nCeO2 at 1000 mg kg(-1) had significantly less Ca but more P and Cu, while pods from 100 mg kg(-1)nZnO had more Zn, Mn, and Cu.

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Silver nanoparticles (Ag NPs) are commonly added to various consumer products and materials to impair bacterial growth. Recent studies suggested that the primary mechanism of antibacterial action of silver nanoparticles is release of silver ion (Ag(+)) and that particle-specific activity of silver nanoparticles is negligible. Here, we used a genome-wide library of Escherichia coli consisting of ∼4000 single gene deletion mutants to elucidate which physiological pathways are involved in how E.

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With the increased use of engineered nanomaterials such as ZnO and CeO₂ nanoparticles (NPs), these materials will inevitably be released into the environment, with unknown consequences. In addition, the potential storage of these NPs or their biotransformed products in edible/reproductive organs of crop plants can cause them to enter into the food chain and the next plant generation. Few reports thus far have addressed the entire life cycle of plants grown in NP-contaminated soil.

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