Phage Genome Diversity in a Biogas-Producing Microbiome Analyzed by Illumina and Nanopore GridION Sequencing.

The microbial biogas network is complex and intertwined, and therefore relatively stable in its overall functionality. However, if key functional groups of microorganisms are affected by biotic or abiotic factors, the entire efficacy may be impaired. Bacteriophages are hypothesized to alter the steering process of the microbial network.

Draft Genome Sequence of a New Bacterium Isolated from Anaerobic Digestion of Biomass.

Here, we present the genome sequence and annotation of the novel bacterial strain HV4-5-C5C, which may represent a new genus within the family (order ). This strain is a potential keystone species in the hydrolysis of complex polymers during anaerobic digestion of biomass.

Complete Genome Sequence of a New sp. Isolated from Anaerobic Digestion and Biomethanation.

Here, we present the genome sequence and annotation of the bacterial strain HV4-5-A1G, a potentially new species. Based on its genomic data, this strain may act as a keystone microorganism in the hydrolysis of complex polymers, as well as in the different acidogenesis and acetogenesis steps during anaerobic digestion.

Complete Genome Sequence of a New Bacterium Isolated from Anaerobic Biomass Digestion.

Here, we present the genome sequence and annotation of HV4-6-C5C, a bacterial strain isolated from a mesophilic two-stage laboratory-scale leach bed biogas reactor system. Strain HV4-6-C5C may represent a new genus of the family and may have a key role in acidogenesis and acetogenesis steps during anaerobic biomass digestion.

Complete Genome Sequence of a New Ruminococcaceae Bacterium Isolated from Anaerobic Biomass Hydrolysis.

A new bacterium, strain HV4-5-B5C, participating in the anaerobic digestion of grass, was isolated from a mesophilic two-stage laboratory-scale leach bed biogas system. The draft annotated genome sequence presented in this study and 16S rRNA gene sequence analysis indicated the affiliation of HV4-5-B5C with the family outside recently described genera.

Microbial communities involved in biogas production exhibit high resilience to heat shocks.

We report here the impact of heat-shock treatments (55 and 70 °C) on the biogas production within the acidification stage of a two-stage reactor system for anaerobic digestion and biomethanation of grass. The microbiome proved both taxonomically and functionally very robust, since heat shocks caused minor community shifts compared to the controls, and biogas yield was not decreased. The strongest impact on the microbial profile was observed with a combination of heat shock and low pH.

Demand-driven biogas production from sugar beet silage in a novel fixed bed disc reactor under mesophilic and thermophilic conditions.

A newly developed fixed bed disc reactor (FBDR) which combines biofilm formation on biofilm carriers and reactor agitation in one single system was assessed for its applicability to demand-driven biogas production by variable feeding of sugar beet silage. Five different feeding patterns were studied at an organic loading of 4gLd under mesophilic and thermophilic conditions. High methane yields of 449-462Lkg were reached.

Production, characterization, and biogas application of magnetic hydrochar from cellulose.

Hydrothermal carbonization (HTC) produces carbon-rich nano-micro size particles. In this study, magnetic hydrochar (MHC) was prepared from model compound cellulose by simply adding ferrites during HTC. The effects of ferrites on HTC were evaluated by characterizing solid MHC and corresponding process liquid.