Comorbidities of COVID-19 Patients.

The novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) responsible for the coronavirus disease outbreak initiated in 2019 (COVID-19) has been shown to affect the health of infected patients in a manner at times dependent on pre-existing comorbidities. Reported here is an overview of the correlation between comorbidities and the exacerbation of the disease in patients with COVID-19, which may lead to poor clinical outcomes or mortality. General medical issues are also reviewed, such as the types of symptoms present in people infected with SARS-CoV-2, the long-term effects of COVID-19 disease, and the types of treatment that are currently used.

On the Stability of Glutaraldehyde in Biocide Compositions.

Glutaraldehyde (GA) is used as biocide in hospitals. Recent public investigations on the chemical composition of biocides used in Romania have in some cases found GA, as a key ingredient, to be apparently diluted. However, these data did not explicitly consider the complex chemical equilibria inherent to GA.

PC2 Ovotransferrin: Characterization and Alternative Immunotherapeutic Activity.

Characterization and evaluation of immunotherapeutic potential of ovotransferrin PC2 (OTf PC2) were performed in this study. The ovoprotein was obtained from egg white from hens immunized with bacterial antigens, pathogenic for humans. For the negative control samples, OTf was extracted from eggs collected from Specific Pathogen-Free (SPF) hens and purified by affinity chromatography on Protein G-agarose column with two eluting peaks: I, representing ovalbumin, and II, ovotransferrin.

Growth factors and beta-tricalcium phosphate in the treatment of periodontal intraosseous defects: A systematic review and meta-analysis of randomised controlled trials.

Objective: To evaluate the effectiveness at different points in time, of recombinant human platelet-derived growth factor-BB (rhPDGF-BB) coated onto a beta-tricalcium phosphate (β-TCP) carrier compared to β-TCP alone, or to recombinant human growth/differentiation factor-5 (rhGDF-5) adsorbed onto a β-TCP scaffold in intraosseous periodontal defects.

Design: A digital search for randomised controlled trials (RCTs) was conducted on MEDLINE/PubMed. The quality of reporting and the risk of bias of the included RCTs were assessed using the CONSORT guidelines and the Cochrane risk of bias tool.

The dentist's operating posture - ergonomic aspects.

The practice of dentistry involves laborious high finesse dental preparations, precision and control in executions that require a particular attention, concentration and patience of the dentist and finally the dentist's physical and mental resistance. The optimal therapeutic approach and the success of practice involve special working conditions for the dentist and his team in an ergonomic environment. The meaning of the posture in ergonomics is the manner in which different parts of the body are located and thus the reports are established between them in order to allow a special task execution.

Implant overdenture and Locator system in edentulous patient with severely resorbed mandible - a case report.

Clinical studies have revealed that the main objective of implants in the edentulous jaw is to provide support for fixed prostheses or to stabilize complete dentures. Various attachment systems were developed for universal use in partially and completely edentulous patients such as clasps, cone-shape telescope copings, magnets, bar systems, locators. The aim of this case report is to present the Locator attachment that does not use the splinting of implants.

Retrieval analysis on dental implants biointegration phases.

This study was developed for evaluating the possible causes of losing primary stability of dental implants and to find possible associations between implant designs or implant surface treatment and the quality of surrounding bone tissue. There have been evaluated five CP (Commercial Products) titanium dental implants having different shapes and surface morphology. All the implants have been extracted between three and six month from insertion time due to the loosening of primary stability.

Immunodiagnosis of Trichinella infection in the horse.

From 1998 to 2000, 5,267 horse sera were collected from several Trichinella regions in Romania. Sera were initially screened in laboratories in Romania, Serbia and Italy with an ELISA and a Western blot (Wb) using an excretory/secretory (ES) antigen and several conjugates (protein A, protein G, and sheep or goat anti-horse). Differences in serology results were obtained among the different conjugates and also between ELISA and Wb.

The lateral flow card test: an alternative method for the detection of Trichinella infection in swine.

A novel lateral flow card (TS-Card pork) test was developed for the serological detection of Trichinella infected pigs. Based on extensive studies performed in Romania during 1999-2000 this test proved to be highly specific sensitive, rapid (3-12 minutes) and easy to use (no need for laboratory facilities). It can be used both for the detection of Trichinella infection in carcasses and for epizooliological studies using a variety of samples including whole or dried blood, serum, or tissue fluids.

Whole Blood, Serum, and Tissue Fluids in an Enzyme Immunoassay for Swine Trichinellosis.

Two enzyme immunoassays (EIA) for trichinellosis in swine (a research version and a commercial kit obtained from LMD Agro-Vet) were used to test pig whole blood, serum, and tissue fluids as sample sources. Five pigs inoculated with 2,500 Trichinella spiralis were bled weekly and then sacrificed after 42 days. Blood and serum samples from each sampling time and tissue fluids collected at slaughter were tested in both EIA formats.

The epidemic of human immunodeficiency virus infection in Romanian children.

Between June 1989 and May 1991, 29,020 subjects were tested for anti-human immunodeficiency virus (HIV) antibodies; 1079 were seropositive. A big discrepancy existed between the high seroprevalence in the group 0-3 yr of age, 11.22%, and that of 0.

Evaluation of efficacy of pooled sera in a human immunodeficiency virus antibody prevalence in population surveys.

The pooling of five individual serum samples for the detection of human immunodeficiency virus type 1 (HIV-1) antibodies was examined to assess whether testing pooled versus individual sera was technically feasible. Detection of HIV-1 antibodies was performed using a competitive enzyme immunoassay (EIA) Wellcozyme HIV Recombinant commercially available kit. Positive, weak positive and negative sera or HIV-1 antibody from Wellcozyme HIV Recombinant, Du Pont ELISA, SERODIA HIV Mast Diagnostica and from "Stefan S.

[Parodonto-prophylactic restoration of occluding stops by dental bridges].

The authors have studied data from the specialized literature and evaluated their personal experience for identifying the positive and the negative aspects of current methods for the restoration of occluding stops. The study has evidenced that the method employing a median articulatory device and the galvanic model of the antagonist teeth can be used for the biological integration at a higher level of efficiency of dental bridges which are intended to restore occluding stops. Superior functional parameters are achieved.

Epstein-Barr virus (EBV). X. Incidence of EBV antibodies in patients with malignant tumors.

Serum samples from 31 patients with various types of malignancies, 18 patients with different viral infections and 6 healthy subjects as controls, were tested by indirect immunofluorescence (IF) method for antibodies against viral capsid antigens (VCA) and the presence of active EBV infection. EBV antibodies anti-VCA were detected in 19 patients with tumors, in 8 patients with viral infections and in 2 healthy subjects. EBV active infection was found out in 9/19, 3/8 and 0/2 EBV anti-VCA positive patients with malignancies, different viral infections and healthy subjects respectively.

Marek's disease (MD). XX. Incidence and epizootology of MD in broiler flocks.

The frequency of Marek's disease in broiler chickens as measured by gross evidence of tumors at processing differs markedly among flocks and between poultry farm origin. Organs affected in broilers, in order of frequency of tumors, were proventriculus (57.6%), spleen (42.

Epstein-Barr virus (EBV). VII. Established lymphoid cell line (IVPat-88) obtained from synovial fluid of a patient with aseptic arthritis.

Attempts have been made to culture mononuclear cells from synovial fluid of 8 patients with arthropathy, and have led to the development of the lymphoid cell line IVPat-88. Cell line has been propagated by serial passages for more than 14 weeks in continuous culture. The cells grew as single, free-floating individuals, or in dense clumps without adherence to glass or plastic surface.

Epstein-Barr virus (EBV). VI. Incidence of EBV antibodies in patients with malignant lymphoproliferative diseases.

Serum samples from 52 patients with malignant lymphoproliferative diseases and 12 clinically healthy subjects were tested by indirect immunofluorescence (IF) reaction for the presence of specific EB antibodies anti-VCA and active EBV infection. The tests revealed EB antibodies anti-VCA in 32 patients with lymphoproliferative diseases and in 2 clinically healthy subjects and active EBV infection in 18/32 and in 0/2 EB anti-VCA positive patients and clinically healthy subjects, respectively.

Epstein-Barr virus (EBV). V. Incidence of EBV antibodies in patients with rheumatic diseases.

Serum samples from 95 patients with rheumatoid arthritis, 24 patients with other various rheumatic diseases, 50 patients with diabetes mellitus, 34 patients with acute viral infections, 6 patients with infectious mononucleosis, 77 patients with lymphomas and leukemia and 110 blood donors and 24 healthy subjects as normal controls, respectively, were tested by indirect immunofluorescence (IF) reaction for the presence of specific antibodies against Epstein-Barr virus determined viral capsid antigen (anti-VCA) and Epstein-Barr active viral infection. The IF test carried out in acetone-fixed smears of EB-3 cell line revealed EB antibodies anti-VCA in 83.3% of infectious mononucleosis, 61.

Bovine leukemia virus. VII. In vitro replication of virus in bat lung cell culture NBL BLV 2.

Bat lung cell culture NBL 12 Tb 1 Lu chronically infected with cell free bovine leukemia virus (BLV), designed by NBL BLV 2, produces virus proteins and showed the presence of cells with the BLV antigens as well as BLV-induced syncytia for more than 11 years. Virus replication was abundant and increased with passage in NBL BLV 2 cell line and was moderate but decreased with passage in fetal lamb splenic cell line chronically infected with BLV (FLS NI 1228). The amount of virus proteins released in culture fluid by NBL BLV 2 cultures growing on roller bottle was 32.

Turkey herpesvirus. VII. The evolution of Marek's disease in chickens vaccinated at one day of age and challenged at different periods of time after vaccination.

The Single Comb White Leghorn, the Cornell S strain chickens vaccinated at one day of age with cell free Herpesvirus of turkey (HVT) 100 and 1000 focus forming-units (FFU) per bird were raised in isolatores. At different periods of time the vaccinated and control chickens were reared together with chickens with clinical symptoms of Marek's disease previously inoculated with JM-Strain of Marek's disease virus (MDV) in a separate isolation room throughout the experiment. The specific protective effect was 84.

Epstein-Barr virus (EBV). IV. Studies on experimental inactivated vaccine against human EBV infection.

It is well established that EBV-immunogen successfully invoke a strong neutralizing anti-EBV response in experimental animals. The authors report here the successful production of an EBV-immunogen prepared with whole disrupted productive cells of 2 MEBV cell line and with whole virus inactivated with ethylenimine (EI) which produce neutralizing anti-EBV response in experimental animals (calves, sheep, rabbits and SPF chickens).

Epstein-Barr virus (EBV). III. Incidence of EBV antibodies in patients with various tumors.

Serum samples from 553 patients with various tumors, from 26 patients with different viral infections and from 78 clinically healthy subjects were tested by indirect immunofluorescence (IF) reaction for the presence of specific antibodies to Epstein-Barr virus. The test revealed antibodies to EBV in 127 patients with tumors, in 14 patients with viral infections and in 8 healthy persons.