Long-term decrease of CD4+CD45RA+ T cells and impaired primary immune response after post-traumatic splenectomy.

Congenital or acquired absence of the spleen and functional hyposplenism are associated with abnormalities of host defence such as an increased susceptibility to infection with encapsulated bacteria. The effects of the lack of the spleen on cell-mediated immunity are largely unknown. In the present study we have investigated peripheral blood lymphocyte subpopulations in healthy adults who had undergone splenectomy because of severe abdominal trauma > 4 years before the study.

Immunoscintigraphy of inflammatory processes with a technetium-99m-labeled monoclonal antigranulocyte antibody (MAb BW 250/183).

Antigranulocyte immunoscintigraphy with a technetium-99m- (99mTc) labeled monoclonal antigranulocyte antibody (MAb BW 250/183) was performed in 34 in-patients of the departments of accident surgery and internal medicine in order to prove or exclude inflammatory processes. After labeling with 99mTc, 555 MBq, 99mTc-MAb (0.5 mg antibody) were slowly injected intravenously over a period of 5 min.

[Fibrin adhesion of cartilage surfaces].

Basing on encouraging results with the fixation of pure articular cartilage with the fibrin sealant in animal experiments, pure cartilage fragments were replanted in human patients using fibrin sealant. From individual cases biopsies could be taken from macroscopically not well healed areas during arthroscopic examination. The histological results from these cases are presented together with overall clinical experiences.

[Healing of chondral fragments].

Basing on encouraging results with the fixation of pure articular cartilage with the fibrin sealant in animal experiments, pure cartilage fragments were replanted in human patients using fibrin sealant. From individual cases biopsies could be taken from macroscopically not well healed areas during arthroscopic examination. The histological results from these cases are presented together with overall clinical experiences.

Divergent patterns of leucocyte locomotion in experimental post-traumatic osteomyelitis.

Leucocyte locomotion (LL) was evaluated in guinea-pigs with experimental post-traumatic osteomyelitis. Surgical procedure (fracture of the right femur), the number of micro-organisms applied to the fractured site and the time sequence of investigations were kept constant. After the right femur of guinea-pigs had been fractured, the animals were divided into three groups: in group I the fractured site remained uninfected, while in groups 2 and 3 the fractured sites were infected with either Escherichia coli (10(5] or with Staphylococcus aureus (10(4].

A model of experimental post-traumatic osteomyelitis in guinea pigs.

A model of experimental post-traumatic osteomyelitis is described in which the femur of guinea pigs was fractured and infected with E. coli (10(5)) or Staphylococcus aureus (10(4)). Traumatized uninfected animals served as controls.

[Posttraumatic osteomyelitis. Mode of infection, significance of metal implants].

Guniea-pigs of the Duncan-Hartley strain were used in a model of posttraumatic osteomyelitis. One femur had been fractured with or without consequent metal implantation and infected subsequently with 10(7) or 10(5) staphylococcus aureus or E. coli.

Pertrochanteric fractures of the femur. A comparative study of internal fixation with angle nail-plates and flexible condylar nails.

Two methods of internal fixation of pertrochanteric fractures of the femur, angle nail-plates and flexible condylar nails, were compared in a retrospective clinical study, with emphasis on the results and indications as related to the age and physical condition of the patients.

[Acute and chronic experimental posttraumatic osteomyelitis in guinea pigs].

The model of an experimental posttraumatic osteomyelitis is described. Acute and chronic osteomyelitis was produced by inoculation of 10(5) Staph. aureus or E.

[The posttraumatic osteomyelitis in guinea-pigs (author's transl)].

A model of posttraumatic osteomyelitis was developed in 120 Duncan-Hartley guinea-pigs. For this purpose the right femura of all animals were fractured surgically. In 60 guinea-pigs immobilisation of the fractured extremity was omitted, in remaining 60 the medullary space was splinted with intramedullary pin.

[Immunologic examination after posttraumatic splenectomy in childhood (author's transl)].

Immunologic examination was done in 26 patients on to 20 years after posttraumatic splenectomy at the age of 3 to 6 years of life. Immunologic parameters used were: concentration of IgG, IgA, IgM, isoagglutinins, heterophila antibodies, agglutinins against staphylococci E. coli and pertussis, serum opsonins for E.

[Homologous articular cartilage transplantation in animal experiments. Preliminary studies on sheep (author's transl)].

Homologous articular cartilage without subchondral bone instead of the usual osteochondral grafts was transplanted in sheep. The transplants were fixed to the host by a biological tissue adhesive based on fibrinogen clotting. Light- and electronmicroscopic studies showed viable transplants up to 7 months and a stable bony union with the host through endochondral bone formation.

[Behaviour of pertrochanteric fractures of the femur after condylar nailing under dynamical stress (author's transl)].

In continuation of our studies on dynamic tolerance after condylar nailing in pertrochanteric fractures, we have examined the femora of patients who died within the first 2 weeks after surgery and studied their stress tolerance. We appreciated the lack of the weight bearing medial cortical bone and the need for abduction at the fracture site by the behaviour under stress. The results under stress could be compared with an experimental fracture model.

[Splenectomy for traumatic rupture of the spleen in childhood and its sequaelae].

This study was undertaken in order to assess the clinical and immunological consequences of splenectomy for traumatic reasons in childhood. Immunological testing of 22 persons 1 to 20 years subsequent to removal of the spleen for traumatic rupture between the ages of 3 and 6 revealed diminished or absent agglutinins in 10 cases and diminished or absent opsonins to E. coli in 16 cases.

[Stress tolerance of different osteosynthetic procedures in pertrochanteric fractures (author's transl)].

To obtain valid information on stress tolerance of pertrochanteric fractures, we exposed three different methods of osteosyntheses to dynamical stress and the results were compared. The methods of osteosyntheses were application of a 130 degrees angle plate, performing an adjoining osteosynthesis with bone cement or using cephalocondylar nails. The measurements in connection with photographical and roentgenological examinations of osteosyntheses under stress gave the following results: Compared with the other methods treatment with condylar nails gave equal results in static behaviour yet was clearly superior in its dynamic reaction.