Analysis of the Association between the Tgfb1 Gene Haplotype and Liver Diseases in Children.

Transforming growth factor-β1 (TGF-β1), a cytokine with immunosuppressive and pro-fibrogenic activity, is a potential marker of infection, liver transplant rejection, and fibrosis. Its levels in the blood and tissues depend on many factors; however, the role of gene polymorphism is still unclear. In this work, the distribution frequency of three single nucleotide polymorphism (SNP) variants of the gene, namely rs1800469, rs1800470, and rs1800471, was studied in children with end-stage liver disease (ESLD).

Time is of the essence: A single-center experience of hepatic arterial supply impairment management in pediatric liver transplant recipients.

Background: Impairment of hepatic arterial flow including hepatic arterial thrombosis (HAT), hepatic arterial stenosis (HAS), and splenic artery steal syndrome (SASS) is potentially life-threatening complications. The proposed early diagnosis and urgent treatment strategy of graft arterial flow reduction aim to decrease morbidity and mortality.

Methods: Pediatric patients with known hepatic arterial flow impairment were retrospectively reviewed.

Transforming growth factor beta 1 levels in the blood of pediatric liver recipients: Clinical and biochemical correlations.

TGF-β1 is a cytokine with profibrogenic and immunosuppressive activities, which suggest the clinical significance of TGF-β1 for the assessment of graft function after LT. We analyzed the dynamics of TGF-β1 levels in the blood after LDLT in 135 pediatric liver recipients and examined the relationship between the cytokine levels and the laboratory and clinical variables. We found that TGF-β1 levels in the blood of patients with ESLD were lower than that in healthy children of the same age, P = .

Deceased vs living donor grafts for pediatric simultaneous liver-kidney transplantation: A single-center experience.

Introduction: In conditions of limited experience of pediatric simultaneous liver-kidney transplantation (SLKT) using grafts from living and deceased donors, there is a certain need to validate the approach.

Patients: The retrospective study of 18 pediatric patients who received SLKT between 2008 and 2019.

Results: Grafts were obtained from both living and deceased donors.

Effects of Growth Factors on Mobilization of Mesenchymal Stem Cells.

We studied mobilization of bone marrow stem cells the under conditions of boost-therapy with granulocytic CSF and erythropoietin. Immunophenotyping of the peripheral blood was carried out in patients after transplantation of tissue-engineered trachea during the perioperative period. We observed an increase in hemopoietic stem cell pool and appearance of multipotent mesenchymal stromal cells in systemic circulation, their maximum concentration being recorded on days 10-14 after surgery.

Determination of the equilibrium constant of C fullerene binding with drug molecules.

We report a new analytical method that allows the determination of the magnitude of the equilibrium constant of complexation, K, of small molecules to C fullerene in aqueous solution. The developed method is based on the up-scaled model of C fullerene-ligand complexation and contains the full set of equations needed to fit titration datasets arising from different experimental methods (UV-Vis spectroscopy, H NMR spectroscopy, diffusion ordered NMR spectroscopy, DLS). The up-scaled model takes into consideration the specificity of C fullerene aggregation in aqueous solution and allows the highly dispersed nature of C fullerene cluster distribution to be accounted for.

BLOOD TRANSFUSION IN LUNG OPERATIONS.

The authors admit the risks of blood transfusion, as well as the fact that the blood is a limited resource. These conclusions became the basis of the research in order to make an analysis and develop transfusion strategies in the hospital. An assessment of blood components application was performed in specific cases.

Characterization of C60 fullerene complexation with antibiotic doxorubicin.

The aim of this paper was to provide the physico-chemical characterization of a key process leading to amplification of the antitumor effect of antibiotic Doxorubicin (Dox) in vivo and in vitro and occurring at the molecular level through complexation with C60 fullerene. A wide range of physico-chemical tools was used such as UV/Vis and NMR spectroscopies, atomic force microscopy, isothermal titration calorimetry and zeta-potential methods. The unusual thermodynamic behavior of the complexation process was reported, featuring unexpected and, to a certain extent, contradictory experimental observations.

[The relationship between the parameters of mineral density of bone tissue and somatotype in women residing in the Republic of Karelia].

Comprehensive anthropometric and densitometric study using the dual x-ray absorptiometry was conducted to determine the relationship between the mineral density of bone tissue and somatotype in 360 women aged 20 to 87 years, permanently residing in the Republic of Karelia. Significant direct correlation was detected between the somatotype and the amount of mineral substances in the vertebrae, bone mineral density and the area of the lumbar vertebrae. Bone mineral density level of the lumbar vertebrae was higher in women with europlastic and athletic somatotypes, which were characterized by high values of body mass and length, body muscle and fat mass.

[Moot points of diagnosis and treatment of hormone-producing neuroendocrine tumors of pancreas].

Based on literature data and own experience of treatment of 225 patients with neuroendocrine tumors (NET) authors discuss moot points of diagnosis and treatment of this severe category of patients. It is demonstrated that the most appropriate algorithm of diagnosis before surgery is the combination of US with endoscopic US and angiography. Intraoperative diagnosis must be performed with intraoperative US and endoscopic transillumination.

[Changes in heat resistance of the muscle and glycerinated muscle fibers of green toad (Bufo viridis complex)].

Heat resistance of muscles and actomyosin proteins was determined at damaging temperatures of 41, 42, and 43 degrees C in three green toad (Bufo viridis complex) populations from different climatic zones of Uzbekistan. The highest muscle and model resistance to these temperatures was revealed in toads living in the warmest plain regions of the country, while the lowest resistance was specific for animals from the piedmont and mountain regions. The variability of heat resistance of the muscle and models was also the highest in toads of the first group.

[Age dependent changes in cervical region of the spine according to data of nuclear magnetic tomography].

Morphological characteristics of spine cervical region were studied in 132 patients (62 female and 70 male) aged 12-76 years using magnetic resonance tomography. The imaging was performed with the use of T1 and T2--weighting regimes. The study revealed sex- and age-dependent differences in the dimensions of cervical vertebrae.

[Differences in muscle thermostability between diploid and tetraploid species of green toads (Bufo viridis complex) of Middle Asia].

Significant differences were found between diploid (Bufo viridis) and tetraploid (B. danatensis) toad species in their skeletal muscle thermostability. The lower muscle thermostability in tetraploids may be associated with the adaptation of B.

[Effect of the elevated temperature on sex ratio in population of hog slaters (Asellus aquaticus L.)].

Seasonal changes in the abundance and sex ratio in the hog slater were monitored in nature throughout the annual cycle. It was shown that the sex ratio was balanced at a relatively low abundance in the autumn-winter period. The number of males could exceed 1.

[Sensitivity of the hog slater Asellus aquaticus to the toxic effects of heavy metals during different periods of ontogenesis, during different seasons and at different temperatures].

A study was made of acute toxic effects of Cd+, Cu+, Al+, Pb+, Ni+, Mn+ and Zn+ ions on embryos, juveniles, and adults of Asellus aquaticus L. at 19 and 29 degrees C, when examined in different seasons of the same year. The highest sensitivity to heavy metals was displayed by embryos.

[Effect of acute hypothermia on parameters of tetanic contraction of the rat skeletal muscle].

In rats under acute hypothermia, tetanic tension of the medial m. gastrocnemius had a higher amplitude.

[Variability of gamete thermoresistance and heat selection of gametes in frogs and molluscs].

The variability of heat resistance of gametes in amphibians and molluscs has been analysed in terms of the problem of direct thermal selection at presygotic stages of ontogenesis. A high variability in gamete heat resistance was revealed both within the frame of a population of animals and in a particular individual. The variability in gamete resistance of an individual animal was as great as 6-8 fold, whereas that within a population reached 8-10 fold value.

[The effect of preliminary acute hypothermia on the characteristics of the single contraction of rat skeletal muscle].

The time parameters and amplitude of the m.gastrocnemius contraction in rats were studied under conditions of acute hypothermia. The contraction time was found to increase whereas the contraction force decreased in acute hypothermia.

[Use of hydrazine for rapid deblocking of synthetic oligonucleotides].

A rapid method for the removal of protecting groups from synthetic oligodeoxyribonucleotides, obtained by the phosphoroamidite and H-phosphonate methods, including the use of hydrazine solutions has been developed. The combination of this procedure with the application of isopropoxyacetyl N-protecting group and oxalyl ester linkage for the first nucleoside attachment to the polymer support allows to reduce to several minutes the time needed for the full oligonucleotide deprotection.

[The heavy metal content in the soft tissues and shells of specimens of 3 varieties of the Azov and Black Sea mussel Mytilus galloprovincialis].

In representatives of 15 micropopulations of the Black Sea mussels related to three varieties of Mytilus galloprovincialis var. dilatata, var. acrocyrta, var.

[Synthesis and expression in Escherichia coli of the gene for the albumin-binding domain of Streptococcus protein G].

The chemical-enzymatic synthesis of a gene coding for A2B2 repeats of the albumin-binding domain of streptococcal protein G has been accomplished. The codon usage of the natural gene has been modified to adapt an artificial sequence for the efficient translation in E. coli.

Rapid deprotection procedures for synthetic oligonucleotides.

Two new rapid procedures for the full deprotection of synthetic oligonucleotides has been developed. We have successfully used the mixture of ethanolamine and ethanol (1:1) or pure ethanolamine for deprotection of oligonucleotides, prepared by different methods. In the case of oligonucleotides prepared by commonly used beta-cyanoethyl phosphoramidite and H-phosphonates method deprotection takes half an hour at 70 degrees C.

[Synthesis of human proinsulin in Escherichia coli cells].

Expression of the synthetic gene for human proinsulin in E. coli has been investigated. The proinsulin gene has been expressed directly under the control of a synthetic promoter of phage fd DNA and a promoter of tryptophan operon, or using fusions with fragments of some bacterial proteins.