[Post-radiation effect on the interhemispheric asymmetry in EEG and thermography characteristics].

Complex analysis of EEG and thermographic parameters carried out in 10 healthy subjects and 34 patients, Chernobyl clean-up participants revealed a correlation between EEG and brain temperature changes in the baseline state and during mental arithmetic. During cognitive activity the maximal increase in the average EEG coherence and temperature shifts in healthy subjects were observed in the left frontotemporal and right parietotemporal areas. In patients changes in both parameters under study were most pronounced, the interhemispheric relations were impaired.

[Use of transmembrane current harmonics originating from electrostriction for clarifying mechanisms of ion transport in prepartions of sodium-potassium ATPase].

The mechanisms of generation of transmembrane current harmonics arising due to electrostriction of the bilayer upon applying an alternating electric voltage to sodium/potassium ATPase sorbed on the support was studied. It is discussed what information can be obtained by measurements of transmembrane current harmonics to clarify the mechanism of ion transport in sodium/potassium ATPase preparations. It was shown that for studying the current harmonics upon electrostriction, the preparations should be sorbed on bilayer lipid membranes with a high modulus of elasticity.

[Generation of capacitance current harmonics during electrostriction of inhomogeneous bilayers].

The mechanisms of generation of capacitance current harmonics arising from bilayer electrostriction are investigated theoretically in the case when fixed charges or dipoles are located inside the bilayer. If the elastic properties of the bilayer are inhomogeneous in a transversal direction, these charges or dipoles affect substantially the amplitude A2 of the second current harmonic and accordingly the magnitude of compensatory constant voltage VC, which shold be applied to the membrane to reduce A2 to zero. In membranes whose Young's modulus is minimum at the center of bilayer, the dependence of VC on depth of immersion of the charge xA into the bilayer represents a function with alternating signs, which is reduced to zero at the center of bilayer.

Microbial pathogens and apoptotic anticancer therapy.

In spite of tremendous efforts to control cancer, the mortality associated with this disease has been increasing in developed countries in the recent decades. Inadequate efficiency of existing therapeutic regimens and the rise of multi-drug resistant cancer cells are the main factors which require a broadening of investigations into novel anticancer strategies. Enhancement of apoptosis in tumours has been suggested recently as a new anticancer strategy.

[Current-voltage characteristics of bilayer lipid membranes formed on the substrates].

Current-voltage characteristics (CVC) of bilayer lipid membranes formed on the support (s-BLM) were measured in static mode as well as in cyclic one with the period 33 s. The new cut of the wire from stainless stell SS-10T served as the support. In the range of voltages -0.

[Conductivity fluctuation of lipid bilayer membranes formed on a substrate].

In the range of voltages -0.9-(+)0.9 V the fluctuations of conductivity of bilayer lipid membranes formed on the support (s-BLM) were measured.

[Modeling changes in membrane physical changes during binding of a substrate to membrane proteins].

Change in the physical properties of the bilayer membranes due to binding of the hormone molecules by the membrane proteins are investigated. They are manifested substantially only for definite protein concentration and are determined by the dimensions of the zones of the distorted structure which are located near free receptor molecules as well as the receptor-substratum complex. The mechanisms of membrane and blosensors sensitivity to hormones are discussed.

[Prospects for studying biomembrane elasticity fluctuations].

The fluctuations of membrane elasticity accompanying the substratum binding and dissociation with the macromolecules incorporated into the bilayer can bear some information on constants of binding reactions as well as on the size of distortion zones around incorporated macromolecules as well as on their number in the membrane. The measurement of membrane elasticity fluctuations can be also used for the biosensors containing supported bilayers.

Purification of bacterial exotoxins. The case of botulinum, tetanus, anthrax, pertussis and cholera toxins.

Bacterial protein toxins and their fragments have been isolated and purified for various reasons, including the development of efficient vaccines and for methods of identification of bacterial agents causing disease. This activity continues today but a new area of bacterial protein toxin research has recently emerged. Since it was shown that toxin molecules comprise several types of biological activity within their structural domains, it was suggested to use these domains (and their combinations) as biochemical tools for developing novel agents for disease imaging and and/or relieving.

[Kinetics of heat processes in human muscle].

Measurements of deep temperature in muscle are conducted by means of microwave radiothermometer which allow one to separate contributions of different intramuscle processes: that of the contractile system, bloodflow, heat production due to metabolic processes during muscle work. The efficiency coefficient of the contractile system is not less than 33%, bloodflow and metabolism induce equal temperature increase. A mathematical model is proposed to calculate from noninvasive experimental data the blood flow dynamics during and after different muscle loads.

[Luminescence, induced by UV-irradiation, of intact human skin].

UV-C induced chemiluminescence of human skin was investigated in vivo by means of an image-producing system using photon-counting camera and computer PERICOLOR-1000. Luminescence after UV-C application is shown to decline nonexponentially, the parameters of the decline depending on irradiation dose, skin specimen and previous irradiations. If the same skin specimen is irradiated second time 3-36 hours after the first irradiation, the luminescence decline time increases from one-two minutes to more than half an hour.

[The nature of fluctuations in spontaneous otoacoustic emission].

The nature of fluctuations of sound pressure during spontaneous otoacoustic emission is studied. The phenomenological mechanism of realization of positive feedback in cochlear is proposed. It is based on usage of hair cells ionic channels.

[Aggregation of membrane proteins of E. coli cells after treatment with singlet oxygen].

It was found that interaction of 1O2 with bacterial membranes of E. coli cells results in covalent binding and aggregation of membrane proteins. This process was shown to be inhibited by water-soluble free radical scavengers, e.

[Ion fluxes during propagation of depression wave].

A mathematical model of spreading depression wave is proposed and ionic fluxes are calculated.

[Immunodiagnosis of AIDS using preparations of the recombinant antigen of the surface protein of the HTLV-III virus].

A technological scheme has been developed for purification of recombinant antigen of surface protein (ASP) of the causative virus of AIDS from Escherichia coli cells carrying plasmid pL2 which codes for the synthesis of a hybrid polypeptide consisting of phage lambda N protein (59 amino acid residues) and a fragment of SP (env) of HIV virus (569 a.r.).

[Effect of SOS repair in enterobacteria on their interaction with the complement system].

The interaction of E. coli (serovar 0124) and its rec A-mutants with serum complement resulting in the alternative pathway activation was studied. Bacteria VT1240 (original smooth strain), VT1241 (rough mutant) and VT 2240 (recA56 mutant) were shown to be complement-sensitive when treated with 1.

[Evaluation of heat production during propagation of the depression wave].

A mathematical model is proposed to describe a heat wave accompanying the spreading cortical depression wave in the large brain. The model takes into account heat production of neurons, thermal transmission with thermoconductivity and volume thermal losses. The model fits well for the wave shape and permits one to estimate heat production which is about 4-20 J/g X min.

[Mechanism of the anti-tumoral effect of the blastolysin fraction isolated from Lactobacillus bulgaricus].

A blastolysin fraction, isolated from lysosyme lysates of Lactobacillus Bulgaricus strain 51 by gel chromatography, was shown to markedly inhibit the growth of sarcoma S-180. A single administration of the agent was followed by an insignificant decrease in tumor growth rate, while repeated treatment induced a complete regression of tumor in 15% of animals and resulted in a prolonged suppression of tumor growth in the others. Cells isolated from metastases which developed after the withdrawal of the drug retained susceptibility to fraction 2 of blastolysin.