Ionizing radiation increases the endothelial permeability and the transendothelial migration of tumor cells through ADAM10-activation and subsequent degradation of VE-cadherin.

Background: We analyzed the changes in permeability of endothelial cell layers after photon irradiation, with a focus on the metalloproteases ADAM10 and ADAM17, and on VE-cadherin, components crucial for the integrity of endothelial intercellular junctions, and their roles in the transmigration of cancer cells through endothelial cell monolayers.

Methods: Primary HUVEC were irradiated with 2 or 4 Gy photons at a dose rate of 5 Gy/min. The permeability of an irradiated endothelial monolayer for macromolecules and tumor cells was analyzed in the presence or absence of the ADAM10/17 inhibitors GI254023X and GW280264X.

The Triple-negative Breast Cancer Cell Line MDA-MB 231 Is Specifically Inhibited by the Ionophore Salinomycin.

Background/aim: Tumour cells of the profile CD44/CD24 have a high tumorigenic potential. Salinomycin can specifically inhibit the growth of these cells. Herein, we investigated the effects of salinomycin on the viability and migration of triple negative breast cancer cells.

The increased adhesion of tumor cells to endothelial cells after irradiation can be reduced by FAK-inhibition.

Background: Radiotherapy is administered in more than 60% of all solid tumors. Most patients are cured but a significant number develops local recurrences or distant metastases. The question arises if irradiation might influence the metastatic process.

Robo1 and vimentin regulate radiation-induced motility of human glioblastoma cells.

Glioblastoma is a primary brain tumor with a poor prognosis despite of many treatment regimens. Radiotherapy significantly prolongs patient survival and remains the most common treatment. Slit2 and Robo1 are evolutionarily conserved proteins involved in axon guidance, migration, and branching of neuronal cells.

Automated Multichamber Time-lapse Videography for Long-term Observation of Migrating Cells.

Aim: To observe and document the migration of living cells by time-lapse videography, we constructed a low-budget system based on a common inverted microscope.

Materials And Methods: Long-term observation of six-well plates is enabled through maintenance of cell culture conditions (5% CO in air at 37°C). Points of interest can be revisited in definable intervals with <1 μm repositioning error.

The therapeutic effect of photon irradiation on viable glioblastoma cells is reinforced by hyperbaric oxygen.

Background: Hyperbaric oxygen (HBO) seems to intensify the effect of ionising radiation. We investigated whether HBO combined with irradiation decreases the capability of U251 glioblastoma cells for relapse and metastasis.

Materials And Methods: Cells were treated with O2 at 1.