Stimulation of anti-melanoma immune effectors via modified tumour cells exhibiting inhibited IGF-I and low CD9.

Modified melanoma cells (B16-F0.MOD) characterized by inhibited IGF-I, CD9 low but not their wild-type counterparts (B16-F0.WT), IGF-I positive, CD9 high, were shown to be immunogenic for syngeneic hosts.

Modulation of the primary and the secondary antibody response by tobacco smoke condensates.

Cigarette smoke condensate administered to C57BL/6 mice led to a decrease in the primary antibody response to OVA (hen egg albumin) antigen. Selenium (Se)-supplementation allowed to relieve significantly this inhibition. Moreover, even being not supplemented with Se, a preparation was found devoid of inhibitory effects.

In vitro and in vivo immunotoxic and immunomodulatory effects of nonsupplemented and selenium-supplemented cigarette smoke condensate.

Cigarette smoke condensate has been evaluated for its in vitro and in vivo immunotoxic and immunomodulatory properties. It was found that cigarette smoke condensate used in vitro at concentration from 6.6 to 20 microg/ml exerted pronounced inhibitory effects upon cell surface antigen-presenting major histocompatibility complex class I (MHC-I) expression and immunoglobulin (Ig) synthesis.

Adenovirus-mediated anti insulin-like growth factor I gene transfer for the treatment of hepatocellular carcinoma.

We have established a hepatocarcinoma cell line (LFCl2A) that produces voluminous tumors when injected into syngeneic Commentary rats. We have previously shown that when these cells were transfected with an episomal vector expressing the antisense IGFI cDNA the transduced cells partly lost their tumorigenic properties and were able to induce the regression of established hepatocarcinoma in syngeneic animals. In this paper, our aim was to determine if one could substitute the use of episomal expression vector by constructing a recombinant adenoviral vector that should be, in theory, easier to supply to humans.