An integrative view on vacuolar pH homeostasis in Arabidopsis thaliana: Combining mathematical modeling and experimentation.

The acidification of plant vacuoles is of great importance for various physiological processes, as a multitude of secondary active transporters utilize the proton gradient established across the vacuolar membrane. Vacuolar-type H -translocating ATPases and a pyrophosphatase are thought to enable vacuoles to accumulate protons against their electrochemical potential. However, recent studies pointed to the ATPase located at the trans-Golgi network/early endosome (TGN/EE) to contribute to vacuolar acidification in a manner not understood as of now.

Impact of explicit area scaling on kinetic models involving multiple compartments.

Background: Computational modelling of cell biological processes is a frequently used technique to analyse the underlying mechanisms and to generally understand the behaviour of these processes in the context of a pathway, network or even the whole cell. The most common technique in this context is the usage of ordinary differential equations that describe the kinetics of the relevant processes in mechanistic detail. Here, it is usually assumed that the content of the cell is well-stirred and thus homogeneous - which is of course an over-simplification, but often worked in the past.

Computational systems biology of cellular processes in Arabidopsis thaliana: an overview.

Systems biology strives for gaining an understanding of biological phenomena by studying the interactions of different parts of a system and integrating the knowledge obtained into the current view of the underlying processes. This is achieved by a tight combination of quantitative experimentation and computational modeling. While there is already a large quantity of systems biology studies describing human, animal and especially microbial cell biological systems, plant biology has been lagging behind for many years.

Robustness of frequency vs. amplitude coding of calcium oscillations during changing temperatures.

Intracellular calcium oscillations have been widely studied. It is assumed that information is conveyed in the frequency, amplitude and shape of these oscillations. In particular, calcium signalling in mammalian liver cells has repeatedly been reported to display frequency coding so that an increasing amount of stimulus is translated into an increasing frequency of the oscillations.