Publications by authors named "Parvathy Madhusoodanan Sujatha"
In insect genome editing CRISPR/Cas9 is predominantly employed, while the potential of several classes of Cas enzymes such as Cas12a largely remain untested. As opposed to Cas9 which requires a GC-rich protospacer adjacent motif (PAM), Cas12a requires a T-rich PAM and causes staggered cleavage in the target DNA, opening possibilities for multiplexing. In this regard, the utility of Cas12a has been shown in only a few insect species such as fruit flies and the silkworm, but not in non-model insects such as the fall armyworm, Spodoptera frugiperda, a globally important invasive pest that defies most of the current management methods.
View Article and Find Full Text PDFEmbryonic microinjection of ribonucleoprotein complex (Cas9+sgRNA) of white gene in melon fly, Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae) produced white eye phenotype.
Sanjay Kumar Pradhan Ashok Karuppannasamy Parvathy Madhusoodanan Sujatha Bhargava Chikmagalur Nagaraja Anu Cholenahalli Narayanappa
Arch Insect Biochem Physiol
December 2023
Article Synopsis
- The melon fly (Zeugodacus cucurbitae) is a significant pest affecting cucurbit crops, leading to considerable yield loss and economic impact.
- The CRISPR/Cas9 system is a powerful genome editing tool that can target specific genes to create stable genetic changes, which can help manage pest traits more efficiently without needing extensive genetic data.
- In this study, researchers successfully used CRISPR/Cas9 to mutate the white gene in melon flies, resulting in a white eye phenotype, which aids in understanding gene function and could lead to innovative pest control methods like precision guided sterile insect technique (pgSIT).