Publications by authors named "Parul Nisha"
Article Synopsis
- The RNAi pathway in the yeast Schizosaccharomyces pombe plays a critical role in forming heterochromatin, but it's unclear if this process is the same in Drosophila.
- Using chromatin immunoprecipitation (ChIP)-seq, researchers found that Argonaute2 (AGO2) in Drosophila mainly localizes to euchromatin and interacts with chromatin insulators like CTCF and CP190, rather than with areas producing siRNA.
- Mutations in AGO2, CTCF, or CP190 disrupt chromosomal looping and affect gene expression, suggesting AGO2’s RNAi-independent role aids in organizing transcriptional domains in the genome.
While heterochromatic gene silencing in cis is often accompanied by nucleosomal compaction, characteristic histone modifications, and recruitment of heterochromatin proteins, little is known concerning genes silenced by heterochromatin in trans. An insertion of heterochromatic satellite DNA in the euchromatic brown (bw) gene of Drosophila melanogaster results in bwDominant (bwD), which can inactivate loci on the homolog by relocation near the centric heterochromatin (trans-inactivation). Nucleosomal compaction was found to accompany trans-inactivation, but stereotypical heterochromatic histone modifications were mostly absent on silenced reporter genes.
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