A Validated Stability-Indicating HPLC-PDA Method for Tolnaftate: Identification, Characterization and In Silico Toxicity Predictions of Major Degradation Products.

Simple and rapid stability indicating High Performance Liquid Chromatography-Photo Diode Array (HPLC-PDA) method was developed and validated for the estimation of tolnaftate in the presence of its forced degradation products. The method employed SunQSil C18 column (250 mm × 4.6 mm, 5 μm) as stationary phase and acetonitrile:water (85:15, v/v) as mobile phase.

Evaluation of BNT162b2 vaccine effectiveness in Malaysia: test negative case-control study.

There is a notable lack of vaccine effectiveness studies using test-negative case-controlled approach in low- and middle-income countries which have different logistic, demographic and socio-economic conditions from high-income countries. We aimed to estimate the effectiveness of BNT162b2 vaccine against COVID-19 infection over time, intensive care unit admission, severe or critical disease and death due to COVID-19. This study was conducted in the resident population of Labuan aged ≥18 years who had been tested for SARS-CoV-2 by Reverse-Transcriptase Polymerase Chain Reaction between 1 March 2021 and 31 October 2021.

Biotransformation of Cranberry Proanthocyanidins to Probiotic Metabolites by Enhances Their Anticancer Activity in HepG2 Cells .

This study was designed to unravel the role of in the bioconversion of cranberry proanthocyanidins and cytotoxicity of resulting metabolites to hepatocellular carcinoma HepG2 cells. Crude (CR) and flavonol+dihydrochalcone- (FL+DHC-), anthocyanin- (AN-), proanthocyanidin- (PR-), and phenolic acid+catechin- (PA+C-) rich fractions were subjected to fermentation with at 37°C for 12, 24, and 48 h under anaerobic conditions. The major metabolites produced by bioconversion of polyphenols were 4-hydroxyphenylacetic acid, 3-(4-hydroxyphenyl)propionic acid, hydrocinnamic acid, catechol, and pyrogallol.

Molecular Docking, In-Silico ADMET Study and Development of 1,6- Dihydropyrimidine Derivative as Protein Tyrosine Phosphatase Inhibitor: An Approach to Design and Develop Antidiabetic Agents.

Background: 1,6-Dihydropyrimidine exerts notable pharmacological efficiency and emerged as integral backbones for treatment of type-II diabetes mellitus. To optimize the in vitro and In-silico study we carried out on substituted 1,6-Dihydropyrimidine. The objective of the present study is to evaluate the binding interaction of 1,6-Dihydropyrimidine compounds with Protein Tyrosine Phosphatase (PTP1B) enzyme and also check ADME/T properties of best scored compounds.

Kinase-targeted cancer therapies: progress, challenges and future directions.

The human genome encodes 538 protein kinases that transfer a γ-phosphate group from ATP to serine, threonine, or tyrosine residues. Many of these kinases are associated with human cancer initiation and progression. The recent development of small-molecule kinase inhibitors for the treatment of diverse types of cancer has proven successful in clinical therapy.

Erythrocyte Alloimmunization and Autoimmunization among Blood Donors and Recipients visiting a Tertiary Care Hospital.

Introduction: The ultimate aim of pretransfusion testing is the acceptable survival of donor red cells in recipient's body and antibody detection plays a critical role in achieving the same. The cornerstone of antibody detection method is detecting an unexpected antibody as against the expected antibodies of ABO blood group system. Autoantibodies can also interfere with the detection of clinically significant alloantibodies.

Anti-Inflammatory Activity of Haskap Cultivars is Polyphenols-Dependent.

Haskap (Lonicera caerulea L.) berries have long been used for their health promoting properties against chronic conditions. The current study investigated the effect of Canadian haskap berry extracts on pro-inflammatory cytokines using a human monocytic cell line THP-1 derived macrophages stimulated by lipopolysaccharide.

Optimization of β-cyclodextrin-based flavonol extraction from apple pomace using response surface methodology.

The present study investigated five cyclodextrins (CDs) for the extraction of flavonols from apple pomace powder and optimized β-CD based extraction of total flavonols using response surface methodology. A 2(3) central composite design with β-CD concentration (0-5 g 100 mL(-1)), extraction temperature (20-72 °C), extraction time (6-48 h) and second-order quadratic model for the total flavonol yield (mg 100 g(-1) DM) was selected to generate the response surface curves. The optimal conditions obtained were: β-CD concentration, 2.

Bio-conversion of apple pomace into ethanol and acetic acid: Enzymatic hydrolysis and fermentation.

Enzymatic hydrolysis of cellulose present in apple pomace was investigated using process variables such as enzyme activity of commercial cellulase, pectinase and β-glucosidase, temperature, pH, time, pre-treatments and end product separation. The interaction of enzyme activity, temperature, pH and time had a significant effect (P<0.05) on release of glucose.

Optimization of dilute acid-based pretreatment and application of laccase on apple pomace.

The present study was aimed to optimize acid-based pretreatment of apple pomace in relation to acid concentration, temperature and reaction time using response surface method with glucose as response variable. In addition, laccase (EC. 1.

Determinants of Overweight and Obesity in Affluent Adolescent in Surat City, South Gujarat region, India.

Background: Obesity is a major global burden. Low levels of physical activity, TV watching, and dietary pattern are modifiable risk factors for overweight and obesity in adolescent.

Objective: The objective of this study was to determine risk factors for overweight and obesity among affluent adolescent, in Surat city in south Gujarat.

Prevalence of hypertension in school going children of Surat city, Western India.

Background: Early diagnosis of hypertension (HT) is an important strategy in its control. Previous studies have documented that hypertension may begin in adolescence, perhaps even in childhood. The purpose of this study was to determine the prevalence of hypertension and risk factors among school going children in Surat city, south Gujarat, India.

Lipid profile in the progeny of parents with ischemic heart disease.

Lipid profile of 50 offsprings of parents with ischemic heart disease and 15 control children aged 5-16 years was studied. The children in both the groups were categorized into 3 sub groups, 5-10, 11-15 & > 15 years. The Serum total cholesterol levels (mean +/- S.

Tear lactoferrin concentration during postoperative ocular inflammation in cataract surgery.

Tear lactoferrin concentration was measured by ELISA technique and followed in 30 patients undergoing cataract surgery. On the first day following surgery, there was a significant decrease in tear lactoferrin concentration followed by a gradual return to the initial values during the postoperative observation period of 7 days. There was an inverse linear relationship between tear lactoferrin concentration and the tear secretion rate measured by a modified Schirmer I test (1 min) suggesting a constant lactoferrin secretion by the tear glands.

Tear film flow and stability in normal Indian subjects.

Tear film flow and stability studies were carried out in healthy population by Schirmer's test-1 and tear film break-up-time (BUT) measurements, respectively. The mean BUT observed was 13.87 +/- 4.

Preserved vision in a case of morning glory syndrome: some pertinent questions.

Morning glory syndrome, an unusual developmental anomaly of the optic nerve head, presenting with funnelled enlargement of the disc with a white dot in the centre and a peripheral vascular pattern along the disc margin, with features of typical entry and exit of retinal vessels on the optic nerve head and exceptionally good vision in the involved eye with old traumatic retinal detachment in the other, in a young Indian girl is described here for its rarity and indifferent clinical presentation. Various factors in its pathogenesis are postulated.

Effect of methyl cellulose on tear film break-up-time in health and disease.

Methyl cellulose instillation enhanced the values of BUT in healthy individuals, as well as in patients with trachoma stage III and chronic conjunctivitis, while no effect was observed in patients with squamous blepharitis.

Tear film flow and stability in acute and chronic conjunctivitis.

Tear film flow and stability studies were carried out in patients suffering from acute and chronic conjunctivitis by performing Schirmer test-I and break-up-time (BUT) measurements. In this clinical study, patients with chronic conjunctival inflammation demonstrated a premature break-up of tear film despite normal amounts of aqueous tears. In acute catarrhal conjunctivitis the values of BUT were within normal limits, whereas the values of Schirmer test-I were significantly higher (t-test, P less than 0.

Study of tear film flow and stability in chronic dacryocystitis.

Tear film flow and stability studies were carried out in patients suffering from chronic dacryocystitis by performing Schirmer test-I and tear film break-up-time (BUT) measurements. The results of Schirmer's test corroborated the theory that a reduced out flow of tears causes a compensatory reduced tear secretion. The results of break-up-time confirm the fact that BUT is independent of outflow of tears in patients with epiphora.

Tear film flow and stability in herpes simplex keratitis and chronic blepharitis.

Tear film flow and stability studies were carried out in patients suffering from herpes simplex keratitis and chronic blepharitis by performing Schirmer test I and tear film break-up-time (BUT) measurements. In herpes simplex keratitis the values of BUT were within normal limits. Values of Schirmer test I were significantly higher (t-test, P less than 0.