Large-scale CRISPR/Cas9 deletions within the WFDC gene cluster uncover gene functionality and critical roles in mammalian reproduction.

Despite 96 million years of evolution separating humans and rodents, 11 closely related reproductive tract-specific genes in humans-, , , , , , , , , , and -and the 13 reproductive tract-specific orthologous genes in mice, form highly conserved syntenic gene clusters indicative of conserved, combined critical functions. Further, despite significant progress toward a nonhormonal male contraceptive targeting the protein encoded by one of these genes, epididymal peptidase inhibitor (EPPIN), and associations found between mutations in and an increased risk of male infertility, neither EPPIN nor any closely related whey acidic protein four-disulfide core (WFDC) gene have been explored functionally. To clarify the involvement of WFDC genes in male fertility, we strategically used CRISPR/Cas9 to generate mice lacking 13, 10, 5, or 4 genes within the cluster and demonstrated that males with deletions of 13, 10, or 4 genes (Wfdc6a, Eppin, Wfdc8, and Wfdc6a) were sterile due to an arrest in spermatogenesis, preventing formation beyond round spermatids.

Animal-Assisted Mental Health Education for Veterinary Students.

Veterinary students often face mental health challenges due to the demanding nature of their studies and the pressures of adopting to their future profession. To address this issue, an animal-assisted education in mental health (AAE-MH) program was developed and implemented at a veterinary school in Hong Kong. The primary goal of the AAE-MH program was to enhance students' mental health literacy, raise awareness of mental health topics, and improve their help-seeking behavior and overall well-being.

Part I: examining the broken history of manual therapy across professions. A survey-based analysis.

The historical development of manual therapy is an area of ongoing debate impacting clinical practice, education, and practice regulations. Primary professions utilizing manual therapy include chiropractic, manual medicine, osteopathy, and physiotherapy. A survey was developed to explore perceptions, experiences, and opinions across professions, and was disseminated globally.

Part II: Beyond Broken Histories: Reframing Professional Identity and the Historical Genesis of Manual Therapy. Interviews across professions.

Background: Perspectives on the historical genesis of manual therapy for chiropractic, manual medicine, osteopathy, and physiotherapy are limited.

Objective: This study sought to identify themes and narratives related to the genesis of manual therapy; the potential of a common root for manual therapy in 19 century Northern Europe; and the potential impact of a current 'broken history' for manual therapy.

Methods: An exploratory phenomenological approach was utilized, based upon structured one-hour interviews of 21 professionals across four professions who had previously completed a survey on the historical genesis of manual therapy.

Development and Integration of Models for Teaching Ram Breeding Soundness Examinations in Veterinary Education.

Proficiency with ram breeding soundness examinations requires competency with palpation, a skill that can be difficult to teach and assess. There are limited small ruminant clinical skills models available, despite the advantages they offer in veterinary education. We developed reusable models for teaching ram breeding soundness examinations, focusing on scrotal assessment and palpation.

Setting Up and Running Online Communities of Practice (CoPs) for Veterinary Educators.

Communities of practice (CoPs) are social systems consisting of individuals who come together to share knowledge and solve problems around a common interest. For educators, membership of a CoP can facilitate access to expertise and professional development activities and generate new collaborations. This teaching tip focuses on online CoPs and provides tips for setting up and running such communities.

Associations between Racing Thoroughbred Movement Asymmetries and Racing and Training Direction.

Background: Racehorses commonly train and race in one direction, which may result in gait asymmetries. This study quantified gait symmetry in two cohorts of Thoroughbreds differing in their predominant exercising direction; we hypothesized that there would be significant differences in the direction of asymmetry between cohorts.

Methods: 307 Thoroughbreds (156 from Singapore Turf Club (STC)-anticlockwise; 151 from Hong Kong Jockey Club (HKJC)-clockwise) were assessed during a straight-line, in-hand trot on firm ground with inertial sensors on their head and pelvis quantifying differences between the minima, maxima, upward movement amplitudes (MinDiff, MaxDiff, UpDiff), and hip hike (HHD).

The behavior and welfare of neglected species: some examples from fish and mammals.

Animal welfare is the state of an animal's body and mind and the level to which its requirements are satisfied. Animal welfare is affected by human decisions and actions. Numerous decisions concerning animals are driven by human desires to enhance their own lives, and some of these decisions may be influenced by self-interest or a strong emphasis on economic factors.

Bringing proteomics to bear on male fertility: key lessons.

Introduction: Male infertility is a major public health concern globally. Proteomics has revolutionized our comprehension of male fertility by identifying potential infertility biomarkers and reproductive defects. Studies comparing sperm proteome with other male reproductive tissues have the potential to refine fertility diagnostics and guide infertility treatment development.

Assessing the impact of draught load pulling on welfare in equids.

About 112 million working equids are the source of income for 600 million people globally. Many equids are used for pulling loads (up to 15,000 kg per day) to transport goods. Most of them are associated with brick kilns, mining, and agriculture industries in developing countries.

Responses to and Reflections on Clinical Skills Teaching and Assessment during COVID-19: A Global Survey.

Clinical skills are traditionally taught face-to-face with a focus on hands-on learning. The COVID-19 pandemic forced institutions to adjust their teaching and assessment. This project investigated how veterinary schools adapted clinical skills teaching and assessment, and identified resulting changes and innovations that will progress clinical skills teaching in the future.

Recognition of emotion and pain by owners benefits the welfare of donkeys in a challenging working environment.

Working donkeys () support human living standards globally. However, there is little information on the effect of human perceptions of emotion and pain on the welfare of working donkeys. We interviewed donkey owners ( = 332) in Pakistan to determine the relationship between human perspectives on donkey sentience: emotions and the ability to feel pain, and the routine working practices that could impact donkey welfare.

Methanogen activity and microbial diversity in Gulf of Cádiz mud volcano sediments.

The Gulf of Cádiz is a tectonically active continental margin with over sixty mud volcanoes (MV) documented, some associated with active methane (CH) seepage. However, the role of prokaryotes in influencing this CH release is largely unknown. In two expeditions (MSM1-3 and JC10) seven Gulf of Cádiz MVs (Porto, Bonjardim, Carlos Ribeiro, Captain Arutyunov, Darwin, Meknes, and Mercator) were analyzed for microbial diversity, geochemistry, and methanogenic activity, plus substrate amended slurries also measured potential methanogenesis and anaerobic oxidation of methane (AOM).

Rapid screening of phenolic compounds in extracts of photosynthetic organisms separated using a C18 monolithic column based HPLC-UV method.

The sourcing of novel bioactive compounds from bioresources has become a sustainability priority for several industrial sectors. Reliable methods are hence needed for the screening and identification of desired molecules in complex extracts. As such, phenolic compounds have attracted increasing interests due to their antioxidant potential.

Influence of exogenous phytohormone supplementation on the pigment and fatty acid content of three marine diatoms.

Diatoms are ubiquitous photosynthetic microorganisms with great potential for biotechnological applications. However, their commercialisation is hampered by production costs, requiring hence optimisation of cultivation methods. Phytohormones are plant growth regulators which may be used to influence physiological processes in microalgae, including diatoms.

Active learning for an evidence-based veterinary medicine course during COVID-19.

Epidemiology is often a challenging course that is not well appreciated by many students learning veterinary medicine. The curriculum for this topic can sometimes be dry, difficult for students to contextualize, and heavy with statistics and mathematical concepts. We incorporated the concepts of epidemiology that are most important for practicing veterinarians and combined these with evidence-based veterinary medicine principles to create a practical course for second-year undergraduate veterinary students.

Serotype Diversity and Antimicrobial Resistance Profile of Isolates From Freshwater Turtles Sold for Human Consumption in Wet Markets in Hong Kong.

Chelonians are recognized as a source of human salmonellosis through direct contact or consumption of their meat. Freshwater turtles sold for food are widely available in wet markets in Asia. In this pilot study, 50 turtles belonging to three species were randomly sampled from wet markets throughout Hong Kong.

Hoof morphometry in a population of lame and nonlame working donkeys in Pakistan.

Background: Hoof morphometry, conformation and shoeing practices have all been associated with lameness in horses. Hoof morphometry in working donkeys in Pakistan has not been objectively measured.

Objectives: To quantitatively assess hoof morphology in donkeys, to identify factors underlying hoof morphology, and interrelationships between conformation, lameness, and shoeing status.

Welfare Concerns for Mounted Load Carrying by Working Donkeys in Pakistan.

Working donkeys () are vital to people's livelihoods. They are essential for carrying goods, however, globally, overloading is one of the primary welfare concerns for working donkeys. We studied mounted load carrying by donkeys and associated factors in Pakistan.

Antioxidant Activity and Carotenoid Content Responses of Three Haematococcus sp. (Chlorophyta) Strains Exposed to Multiple Stressors.

There has been increasing demands worldwide for bioactive compounds of natural origins, especially for the nutraceutical and food-supplement sectors. In this context, microalgae are viewed as sustainable sources of molecules with an array of health benefits. For instance, astaxanthin is a xanthophyll pigment with powerful antioxidant capacity produced by microalgae such as the chlorophyte Haematococcus sp.

Cross-Sectional Questionnaire of Donkey Owners and Farriers Regarding Farriery Practices in the Faisalabad Region of Pakistan.

Quality farriery is essential to ensure donkey welfare, and many intervention programs in low-middle income countries (LMICs) train farriers, and educate owners, regarding the farriery needs of donkeys. It is essential for interventional programs to understand the perspectives of all stakeholders in donkey-owning communities. A cross-sectional questionnaire study was conducted in the Faisalabad region of Pakistan.

Antibacterial Activity and Amphidinol Profiling of the Marine Dinoflagellate (Subclade III).

Microalgae have received growing interest for their capacity to produce bioactive metabolites. This study aimed at characterising the antimicrobial potential of the marine dinoflagellate strain LACW11, isolated from the west of Ireland. Amphidinolides have been identified as cytotoxic polyoxygenated polyketides produced by several species.

Comparative Response of Marine Microalgae to HO-Induced Oxidative Stress.

There have been growing interests in the biorefining of bioactive compounds from marine microalgae, including pigments, omega-3 fatty acids or antioxidants for use in the nutraceutical and cosmetic sectors. This study focused on the comparative responses of five marine microalgal species from different lineages, including the dinoflagellate Amphidinium carterae, chlorophyte Brachiomonas submarina, diatom Stauroneis sp., haptophyte Diacronema sp.

The role of methyl jasmonate in enhancing biomass yields and bioactive metabolites in Stauroneis sp. (Bacillariophyceae) revealed by proteome and biochemical profiling.

The diatom Stauroneis sp. was previously identified as a promising source of fucoxanthin and omega-3 oils. Methyl jasmonate (MJ) supplementation is known to enhance metabolite yields in this species without impacting on growth or photosynthesis.

Cross-Modality Interaction Network for Equine Activity Recognition Using Imbalanced Multi-Modal Data.

With the recent advances in deep learning, wearable sensors have increasingly been used in automated animal activity recognition. However, there are two major challenges in improving recognition performance-multi-modal feature fusion and imbalanced data modeling. In this study, to improve classification performance for equine activities while tackling these two challenges, we developed a cross-modality interaction network (CMI-Net) involving a dual convolution neural network architecture and a cross-modality interaction module (CMIM).