Microbial cell lysate supernatant (CLS) alteration impact on platinum nanoparticles fabrication, characterization, antioxidant and antibacterial activity.

Microbial mediated biological synthesis of nanoparticles is of enormous interest to modern nanotechnology due to its simplicity and eco-friendliness. In the present study, a novel green method for the synthesis of platinum nanoparticles (PtNPs) has been developed using bio-derived product-cell lysate supernatant (CLS) from various microorganisms including Gram-negative bacteria: Pseudomonas kunmingensis ADR19, Psychrobacter faecalis FZC6, Vibrio fischeri NRRL B-11177, Gram-positive bacteria: Jeotgalicoccus coquinae ZC15, Sporosarcina psychrophila KC19, Kocuria rosea MN23, genetically engineered bacterium: Pseudomonas putida KT2440 and yeast: Rhodotorula mucilaginosa CCV1. The biogenic PtNPs were characterized by UV-visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR), transmission electron microscopy (TEM), high resolution transmission electron microscopy (HRTEM), and atomic force microscopy (AFM).