Green Synthesis of Copper Nanoparticles using Rosmarinus officinalis L. Extract Improves the Developmental Competence of Mouse Oocytes during in Vitro Maturation.

An effective approach to enrich the in vitro maturation (IVM) of oocyte medium as one of the main assisted reproduction technologies is the use of antioxidants to minimize oxidative stress. This study examined the effects of copper nanoparticles (CuNPs) synthesized by chemical (Ch-NPs) and green (G-CuNPs) methods on the IVM process of mouse oocytes and the development of the embryo in comparison to control oocytes (without nanoparticles treatment). Hydroalcoholic (G-H-CuNPs) and aqueous (G-A-CuNPs) Rosmarinus officinalis extracts were used for green synthesis.