Long-term assessment of Helicobacter pylori cagA EPIYA motif changes and pathology outcomes in gastric biopsies of dyspeptic patients: 10-year follow-up.

Introduction: Helicobacter pylori exhibit considerable genetic diversity, especially in the cagA gene, which is prone to rearrangement, affecting gastric pathology. This study aims to identify changes in the cagA EPIYA motif patterns and gastric pathology during long-term colonization and to explore how factors such as smoking, alcohol consumption, gender, and age influence these changes.

Methods: Paired formalin-fixed paraffin-embedded (FFPE) gastric biopsies from 100 H.

An Efficient and Easy- to- Use Method for Extraction of DNA from Archival Formalin-Fixed and Paraffin-Embedded Gastric Tissues.

Background & Objective: Formalin-fixed paraffin-embedded archived tissues are useful for the genetic analyses and assessment of some patients' disease history, including infectious diseases. However, there is no established protocol for extracting bacterial DNA from the archived specimens. In this study DNA was extracted from the archived -positive gastric biopsies by some modifications applied to the previously published protocols.

Individual hosts carry H. pylori isolates with different cagA features - motifs and copy number.

Background: H. pylori strains with different genetic contents may infect different or an individual human host. Genetic diversity of cagA is thought to contribute to differences in H.