Hybrid YSGOA and neural networks based software failure prediction in cloud systems.

In the realm of cloud computing, ensuring the dependability and robustness of software systems is paramount. The intricate and evolving nature of cloud infrastructures, however, presents substantial obstacles in the pre-emptive identification and rectification of software anomalies. This study introduces an innovative methodology that amalgamates hybrid optimization algorithms with Neural Networks (NN) to refine the prediction of software malfunctions.

A microfluidic approach to study variations in alkaline phosphatase activity in response to phosphate availability.

Algal growth depends strongly on phosphorus (P) as a key nutrient, underscoring the significance of monitoring P levels. Algal species display a sensitive response to fluctuations in P availability, notably through the expression of alkaline phosphatase (AP) when challenged with P-depletion. As such, alkaline phosphatase activity (APA) serves as a valuable metric for P availability, offering insights into how algae utilize and fix available P resources.

Metagenomics approaches in unveiling the dynamics of Plant Growth-Promoting Microorganisms (PGPM) vis-à-vis Phytophthora sp. suppression in various crop ecological systems.

Phytophthora species are destructive pathogens causing yield losses in different ecological systems, such as potato, black pepper, pepper, avocado, citrus, and tobacco. The diversity of plant growth-promoting microorganisms (PGPM) plays a crucial role in disease suppression. Knowledge of metagenomics approaches is essential for assessing the dynamics of PGPM and Phytophthora species across various ecosystems, facilitating effective management strategies for better crop protection.

Authentication of traditional meat products (Haleem) sold in India: a first report confirming species mislabelling.

Unlabelled: Traditional meat products like Haleem play a pivotal role in the culinary landscapes of Indian consumers, along with high economic value and business potential. Due to anticipated gains associated with adulterating 'Haleem' and constant evasion from regulatory oversight, the susceptibility to adulteration has substantially increased. Furthermore, no reports/surveillance regarding their labelling compliance has been reported.

Eco-friendly K-10 Clay-Mediated [3 + 3] Spiroannulation of Morita-Baylis-Hillman Adduct of Isatin with Anthracene: Synthesis of Green Fluorophore Compounds.

An easy and simple spiroannulation of the Morita-Baylis-Hillman adduct of isatin derivatives with anthracene was achieved in moderate-to-good yields (37-75%). The spiroderivatives synthesized in this work exhibited green fluorescence properties. The reaction occurred in metal-free eco-friendly K-10 clay-mediated conditions.

Predictors of maternal and fetal outcome in severely anemic pregnant mothers: A sequential mixed methods study.

Introduction: Severe anemia in pregnancy increases maternal and neonatal morbidity and mortality in the antenatal period.

Objective: To find out the maternal and fetal outcomes in severely anemic pregnant women and explore the reason for the persistence of severe anemia in pregnant women.

Materials And Methods: Sequential explanatory mixed method study (Quan-Qual) was conducted in the Government Women and Children Hospital for a period of 1 and half years.

Analytical Techniques for Single-Cell Biochemical Assays of Lipids.

Lipids are essential cellular components forming membranes, serving as energy reserves, and acting as chemical messengers. Dysfunction in lipid metabolism and signaling is associated with a wide range of diseases including cancer and autoimmunity. Heterogeneity in cell behavior including lipid signaling is increasingly recognized as a driver of disease and drug resistance.

Duplex real-time PCR assay with high-resolution melt analysis for the detection and quantification of species and in meat products.

Unlabelled: contamination in foods of animal origin is one of the most concerning food safety issues. A duplex, SYBR green-based, real-time PCR assay was developed with high-resolution melting analysis-based differentiation of the genus and . The primers were designed and tested against other related foodborne pathogens.

Tire Condition Monitoring Using Transfer Learning-Based Deep Neural Network Approach.

Monitoring tire condition plays a deterministic role in the overall safety and economy of an automobile. The tire condition monitoring system (TCMS) alerts the driver of the vehicle if the inflation pressure of a particular tire decreases below a specific value. Owing to the high costs involved in realizing this system, most vehicles do not feature this technology as a standard.

Area-to-point regression Kriging approach fusion of Landsat 8 OLI and Sentinel 2 data for assessment of soil macronutrients at Anaimalai, Coimbatore.

Spectral indices-based soil prediction models derived from multispectral datasets are too intricate in terms of accuracy as well as resolution. Complications arise while incorporating multispectral datasets for regional-scale spatial assessment of soil macronutrients. Sporadically satellite image fusion techniques have been used for soil nutrient interpolation to circumvent the complications.

Complete Genome Sequence Analysis of Bbv57, a Promising Biocontrol Agent against Phytopathogens.

Plant growth-promoting rhizobacteria (PGPR) are a group of root-associated beneficial bacteria emerging as one of the powerful agents in sustainable plant disease management. Among the PGPR, Bacillus sp. has become a popular biocontrol agent for controlling pests and the diseases of several crops of agricultural and horticultural importance.

Development and comparative evaluation of droplet digital PCR and quantitative PCR for the detection and quantification of Chlamydia psittaci.

Chlamydia psittaci is a zoonotic pathogen mainly transmitted by psittacine birds and poultry. The low shedding rate of the pathogen in the apparently healthy birds and human clinical cases may result in false-negative results. In the present study, a droplet digital PCR (ddPCR) assay was developed and compared with optimized quantitative PCR (qPCR) for the detection of C.

Authentication of camel meat using species-specific PCR and PCR-RFLP.

In India and some of the African countries, one of the unconventional meats receiving the latest attention in meat adulteration is camel meat. So, the objective of this study was to develop a species-specific PCR based on mitochondrial cytochrome b (CYTB) gene and a PCR-RFLP assay of mitochondrial 12S rRNA to identify camel meat in suspected samples. Known sample of camel meat, samples suspected to be from illegally slaughtered camel and known samples of cattle, buffalo, sheep, goat, pork and chicken were used in the study.

Multiresponse Optimization of HPLC Method: Simultaneous Estimation of Protease Inhibitors and NNRTI in Human Plasma.

Multiresponse optimization approach to develop a simple isocratic, highly sensitive and accurate HPLC method for the simultaneous determination of Efavirenz, Atazanavir, Lopinavir and Ritonavir in human blood plasma along with carvedilol as an internal standard. Optimized the factors (ACN, buffer concentration and flow rate) effecting and interacting with the responses (k1, Rs2,1, Rs3,2 and tR5) applying Central Composite Design a chemometric tool. All the mathematical models as well as response surfaces were defined and derived for the separation using this strategy.

Characterization of PMI-5011 on the Regulation of Deubiquitinating Enzyme Activity in Multiple Myeloma Cell Extracts.

Deubiquitinating enzyme (DUB)-targeted therapeutics have shown promise in recent years as alternative cancer therapeutics, especially when coupled with proteasome-based inhibitors. While a majority of DUB-based therapeutics function by inhibiting DUB enzymes, studies show that positive regulation of these enzymes can stabilize levels of protein degradation. Unfortunately, there are currently no clinically available therapeutics for this purpose.

Lignin-graft-PLGA drug-delivery system improves efficacy of MEK1/2 inhibitors in triple-negative breast cancer cell line.

Few targeted therapies are available for triple-negative breast cancer (TNBC) patients. Here, we propose a novel alkaline-lignin-conjugated-poly(lactic--glycolic acid) (L-PLGA) nanoparticle drug delivery system to improve the efficacy of targeted therapies.  L-PLGA nanoparticles (NPs) loaded with the MEK1/2 inhibitor GDC-0623 were characterized, tested on MDA-MB-231 TNBC cell line and compared with loaded PLGA NPs.

CPProtectides: Rapid uptake of well-folded β-hairpin peptides with enhanced resistance to intracellular degradation.

Cell penetrating peptides (CPPs) have emerged as powerful tools for delivering bioactive cargoes, such as biosensors or drugs to intact cells. One limitation of CPPs is their rapid degradation by intracellular proteases. β-hairpin "protectides" have previously been demonstrated to be long-lived under cytosolic conditions due to their secondary structure.

FluoroCellTrack: An algorithm for automated analysis of high-throughput droplet microfluidic data.

High-throughput droplet microfluidic devices with fluorescence detection systems provide several advantages over conventional end-point cytometric techniques due to their ability to isolate single cells and investigate complex intracellular dynamics. While there have been significant advances in the field of experimental droplet microfluidics, the development of complementary software tools has lagged. Existing quantification tools have limitations including interdependent hardware platforms or challenges analyzing a wide range of high-throughput droplet microfluidic data using a single algorithm.

Green biocide for mitigating sulfate-reducing bacteria influenced microbial corrosion.

In the present investigation, plant extract (PLAE) was used as biocide to control corrosion in the presence of sulfate-reducing bacteria (SRB). Transmission electron microscopy showed the damage of SRB outer cell membrane which lead to cell destruction and disturbed membrane permeability. The scanning electron microscopy also confirmed the cell shrinkage due to green biocide, and energy-dispersive Fourier transform infra-red spectroscopy indicated the decrease in sulfide concentration in the presence of biocide.

Luminescent nanomaterials for droplet tracking in a microfluidic trapping array.

The use of high-throughput multiplexed screening platforms has attracted significant interest in the field of on-site disease detection and diagnostics for their capability to simultaneously interrogate single-cell responses across different populations. However, many of the current approaches are limited by the spectral overlap between tracking materials (e.g.

Microfluidic and Paper-Based Devices for Disease Detection and Diagnostic Research.

Recent developments in microfluidic devices, nanoparticle chemistry, fluorescent microscopy, and biochemical techniques such as genetic identification and antibody capture have provided easier and more sensitive platforms for detecting and diagnosing diseases as well as providing new fundamental insight into disease progression. These advancements have led to the development of new technology and assays capable of easy and early detection of pathogenicity as well as the enhancement of the drug discovery and development pipeline. While some studies have focused on treatment, many of these technologies have found initial success in laboratories as a precursor for clinical applications.

A Goal Unrealized: Patient Empowerment on Hand Hygiene- A Web-Based Survey from India.

Introduction: Each year, millions of patients around the world are affected by Health Care Associated Infections (HCAIs). Understanding and assessing the global burden of HCAI is one of the key areas of work to improve the hand hygiene.

Aim: To assess the patient empowerment and awareness on hand hygiene among online users.