Recognition of G-quadruplex topology through hybrid binding with implications in cancer theranostics.

The selective recognition and imaging of oncogene specific G-quadruplex (GQ) structures holds great promise in the development of diagnostic therapy (theranostics) for cancer and has been challenging due to their structural dynamics and diversity. We report selective recognition of GQ by a small molecule through unique hybrid loop stacking and groove binding mode with turn on far-red fluorescence response and anticancer activity demonstrating the potential implications for GQ-targeted cancer theranostics. Biophysical investigation reveal the turn on far-red emission property of TGP18 for selective recognition of GQ.

Phosphoglycolate phosphatase is a metabolic proofreading enzyme essential for cellular function in .

(Pf) 4-nitrophenylphosphatase has been shown previously to be involved in vitamin B1 metabolism. Here, conducting a BLASTp search, we found that 4-nitrophenylphosphatase from Pf has significant homology with phosphoglycolate phosphatase (PGP) from mouse, human, and yeast, prompting us to reinvestigate the biochemical properties of the enzyme. Because the recombinant PfPGP enzyme is insoluble, we performed an extended substrate screen and extensive biochemical characterization of the recombinantly expressed and purified homolog from (Pb), leading to the identification of 2-phosphoglycolate and 2-phospho-L-lactate as the relevant physiological substrates of PbPGP.

Syntheses of Bipyricorroles and Their Meso-Meso Coupled Dimers.

The metal-templated condensation strategy has been developed for the synthesis of meso-free bipyricorrole complexes. The reactive meso-CH in the monomer complex is further treated with various oxidative coupling reagents such as AgPF, AgOTf, and FeCl. Unlike Ag(I) salts, the FeCl resulted in a meso-meso-linked corrole homologue dimer.