Micronucleus test in reptiles: Current and future perspectives.

Micronucleus (MN) cell counting emerged in 1973-1975 as a valid alternative for characterizing chromosomal damage caused by different agents. It was first described in mammals, but its application was rapidly extended to other vertebrates, mainly fish. However, it was not until 28 years later that this test was implemented in studies on reptiles.

Pesticide-induced alterations in zebrafish (Danio rerio) behavior, histology, DNA damage and mRNA expression: An integrated approach.

To assess the impact of glyphosate and 2,4-D herbicides, as well as the insecticide imidacloprid, both individually and in combination, the gills of adult zebrafish were used due to their intimate interaction with chemicals diluted in water. Bioassays were performed exposing the animals to the different pesticides and their mixture for 96 h. The behavior of the fish was analyzed, a histological examination of the gills was carried out, and the genotoxic effects were also analyzed by means of the comet assay (CA) and the change in the expression profiles of genes involved in the pathways of the oxidative stress and cellular apoptosis.

DNA damage, alterations in the expression of antioxidant enzyme genes and in the histoarchitecture of gill cells of zebrafish exposed to 17-α-ethinylestradiol.

Endocrine disruptors, such as estrogen, are chemical substances with the potential to alter the hormonal balance of organisms. Their origin can be natural or artificial, and they can act at very low doses. The estrogen 17α-ethinylestradiol (EE2) is used worldwide as an oral contraceptive and is a potential contaminant in aquatic ecosystems.

DNA damage and oxidative stress in gill cells of Cnesterodon decemmaculatus exposed to pesticides by runoff source in an agricultural basin.

In our country, great concern exists about diffuse pollution cause by the great use of pesticides in rural environments. A thorough analysis is needed to generate information, know the real situation and thus, be able to make decisions with the purpose of reducing environmental pollution. In situ bioassays have been carried out using Cnesterodon decemmaculatus within limnocorrals located in a surface natural water system that receives rainfall excess flowing from an agricultural basin with a typical crop rotation, including corn, wheat and soy.

Identification and evaluation of antioxidant and reference genes for quantitative real-time PCR in blood of .

The quantitative real-time polymerase chain reaction (qPCR) has been one of the most promising approaches to perform rapid and accurate quantification of DNA in various biological systems. The aim of this study was to standardized the qPCR technique for the analysis of important genes involved in the main routes of antioxidant defense against reactive oxygen species (catalase: and superoxide dismutase: ) and evaluate the stability of different reference genes in blood of hatchlings. The stability of the reference genes, , glyceraldehyde 3-phosphate dehydrogenase () and ribosomal protein L8 () was determined using the comparative ΔCt, NormFinder, geNorm, BestKeeper and RefFinder.

The problem with implementing fish farms in agricultural regions: A trial in a pampean pond highlights potential risks to both human and fish health.

The safety of creating fish farms in agricultural settings was evaluated by growing Piaractus mesopotamicus in a pond, while crops where cultivated in a nearby field under a pesticide application regime typical of the Pampa region. Atrazine, glyphosate and its metabolite, aminomethylphosphonic acid (AMPA), were detected in the water of the pond at concentrations ranging between 92 and 118 μg/L for atrazine, 12 and 221 μg/L for glyphosate and 21 and 117 μg/L for AMPA. Atrazine and malathion were detected in fish muscles at concentrations ranging between 70 and 105 μg/kg for atrazine and 8.

Molecular analysis and bioinformatic characterization of cooper, zinc-superoxide dismutase (Cu/Zn-sod) gene of Caiman latirostris.

Superoxide dismutase (SOD) is an antioxidant enzyme that acts as a component of first-line defense system against reactive oxygen species (ROS). Copper/Zinc superoxide dismutase (Cu/Zn-SOD) is one of the isoforms of SOD enzyme and is sensitive to the exposure of different environmental factors, in different species and tissues. Caiman latirostris is one of the two crocodilian species living in Argentina and no information is available on the molecular and biochemical characteristics of the Cu/Zn-sod gene in this species.

Cypermethrin induction of DNA damage and oxidative stress in zebrafish gill cells.

Cypermethrin (CYP) is a synthetic pyrethroid insecticide, used to control pests in domestic, industrial and agricultural environments. According to recent reports, it is one of the most common contaminants in freshwater aquatic systems. The aim of this study was to evaluate its potential genotoxic effect and the activation of the superoxide dismutase (SOD) and catalase (CAT) systems of adult zebrafish gill cells after in vivo exposure.

Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish.

Cypermethrin (CM), widely used for control of indoor and field pests, is one of the most common contaminants in freshwater aquatic systems. We evaluated CM genotoxicity and the activities of superoxide dismutase (SOD) and catalase (CAT) in retinal cells of adult zebrafish. Histological and immunofluorescence techniques show the presence of apoptotic cells in the zebrafish retina after 9 d of treatment with 0.