Staging of colorectal cancer using serum metabolomics with HNMR Spectroscopy.

Objectives: Determination of stages of colon cancer is done by biopsy usually after surgery. Metabolomics is the study of all the metabolites using LC-MS and HNMR spectroscopy with chemometric techniques. The stages of colon cancer were detected from patients' sera using HNMR.

Comparisons between RT-PCR, real-time PCR, and in vitro globin chain synthesis by α/β ratio calculation for diagnosis of α- from β-thalassemia carriers.

Background: Thalassemia, which may be due to point mutations, translocations, and deletions involving the α or βglobin gene, is the most prevalent single gene disorder in Iran.This study aims to calculate the α/β ratio in normal cases, α- and β-thalassemia carriers by RT-PCR, real-time PCR, and in vitro globin chain synthesis (GCS) in order to establish the most accurate technique to distinguish between α- and β-thalassemia carriers in suspicious cases.

Methods: The α/β ratios were calculated in all samples by RT-PCR, real-time RT-PCR, and in vitro GCS.