Cloning and expression of type D vaccine strain epsilon toxin gene in as a recombinant vaccine candidate.

Background And Objectives: a Gram-positive obligate anaerobic bacterium, is able to form resistant spores which are widely distributed in the environment. is subdivided into five types A to E based on its four major alpha, beta, epsilon and iota toxins. The aim of the present study was cloning and expression of type D vaccine strain epsilon toxin gene.