Publications by authors named "Paraskevi Vasileiou"
FLAER Revealed Normally Expected Non-PNH FLAER-Dim Immature Myeloid Cells (CD117+/CD34-) In Bone Marrow Aspirates and Could Be Utilized as a Marker of Hierarchical Hematopoiesis.
Int J Lab Hematol
October 2024
Article Synopsis
- FLAER (Fluorescently labeled aerolysin) is used to identify PNH clones in blood samples, but there's limited information on its expression in normal bone marrow cells.
- This study analyzed 54 bone marrow samples from various conditions, including PNH and different types of leukemia, to understand FLAER expression in both normal and diseased hematopoiesis.
- Results showed that while FLAER intensity was generally intermediate in stem cells, a specific FLAER-negative subgroup was found in PNH patients, indicating varying expression levels in myeloid precursors across different conditions.
Introduction: Venous blood (VB) sampling for complete blood count (CBC) via venipuncture is the basic method for the daily evaluation of hematological patients. However, several issues during this process, such as venipuncture difficulty and repetitive attempts, may cause pain, phlebitis, hematomas, inadequate sampling, and patient discomfort. Capillary blood (CB) sampling could be an alternative and less painful solution for the patient.
View Article and Find Full Text PDFAims: The causes and diagnosis of 'double-negative' (CD3+CD4-CD8-) T-cell lymphocytosis are not well studied. We aimed to define the causes of double-negative T-cell lymphocytosis in children and adults, and to identify simple clinical and laboratory features that would help to differentiate between the underlying conditions.
Methods: We collected clinical and laboratory data on 10 children and 30 adults with significantly increased peripheral-blood double-negative T-cells (>10% of total lymphocytes).
"Bone marrow aspirate automated counts on hematology analyzers: formulating a scoring system based on hematology parameters, to discriminate reactive versus myelodysplastic syndrome-related bone marrows".
Int J Lab Hematol
August 2019
Introduction: Diagnosis of myelodysplastic syndromes (MDS) is usually challenging. In this context, we have attempted to employ data derived from automated analysis of bone marrow (BM) samples as an ancillary tool for the discrimination between reactive marrow and MDS.
Methods: A total of 101 BM anticoagulated samples referred for flow cytometry (FCM) analysis on the clinical suspicion of MDS had been previously counted in a Mindray BC-6800 hematology analyzer (testing set).