Simulation of limb frostbites in air medium.

The clinical course of frostbites of the limbs was studied in 3 animal species (swine, dog, rabbit) with frostbites of different intensity and duration. Animals of different species exhibited different sensitivity of their limb tissues to cold. The rabbit proved to be the most adequate animal for frostbite simulation.

Effect of local application of superoxide dismutase on dielectric parameters of cooled skin in rats.

The effect of on Changes in dielectric parameters of the skin (modulus of complex dielectric permittivity |e| and dielectric loss tangent tgd) were studied on rats with local surface contact cooling followed by treatment with various cream formulations. Addition of antioxidant superoxide dismutase (SOD) to the cream significantly prevented the shifts in these parameters, which attested to less pronounced changes in the water balance in SOD-treated skin. Application of SOD during the early terms after cooling accelerated wound healing.

Comparative evaluation of the depth of collagen and hyaluronic acid hydrolysis in vitro by collagenase and hyaluronidase preparations.

The molecular weights of collagen and hyaluronic acid solutions after their incubation with collagenase and hyaluronidase were evaluated by capillary viscosimetry. The results indicate high amylolytic activity of collagenase and the absence of proteolytic activity in hyaluronidase in an in vitro system.

[Suspension of autogenic thrombocytes in local treatment of ischemic trophic ulcers of lower extremities].

Based on the results of treatment of 10 patients the authors have shown that the application of the suspension of autogenic thrombocytes increased the frequency and rate of epithelization of ulcers.

UHF-dielectrometry of the urine in prognosis of aggregation stability.

Dielectric permeability of the urine from healthy subjects and patients with various types of urolithiasis was studied using millimeter electromagnetic waves. The urine from healthy subjects and patients with urolithiasis differed in dielectric properties, specific water content, and structure of water. A relationship was revealed between aggregation stability of urine colloids and dielectric properties of the urine in millimeter-wave electromagnetic radiation.

Matrigel increases the rate of split wound healing and promotes keratinocyte ;take' in deep wounds in rats.

The influence of matrigel, a mixture of the components of thebasement membrane, on the wound healing was studied in a modelof experimental wounds in rats. Matrigel was found to increasethe rate of epithelization of split-thickness wounds. The modelof deep wound was developed in which the host animal could notprovide enough migrating and proliferating keratinocytes tocover the wound area.

Dynamics of hydration of cicatricial tissue during local therapy with collagenase preparations.

The components of complex dielectric permeability of cicatricial tissue at the frequency of 55 GHz were studied during local therapy of cicatrices with Fermenkol (a complex of collagenolytic proteases). Electrical characteristics of tissues in the microwave range were interpreted in terms of hydration parameters (total content of water in tissues and the ratio of structured and free water). The rightfulness of interpretations is discussed on the basis of the results of measurements of cell suspension models (native blood and its fractions brought to a standard hematocrit).

[Application of enzymes for treatment of patients with hypertrophic cicatrices].

Treatment of 27 patients by electrophoresis with preparation "Polycollagenase K" was analyzed and it was shown that it gave positive results according to complex assessment by the scale developed by the authors.

The efficacy of cell therapy in the treatment of patients with trophic venous ulcers of the lower limbs.

This paper analyzes the results of cell therapy carried out during multimodality treatment of 23 patients with trophic ulcers of the lower limbs because of varicosity and postthrombotic disease. Twenty-five patients were entered into the control group provided the modern wound dressings Suprasorb. It has been demonstrated that cell therapy applying fibroblasts, strain 11 00/14, noticeably upgrades the treatment efficacy.

[Cell therapy in treatment of trophic ulcers of lower extremities].

The aim of the work was to study the effectiveness of using human embryo fibroblast culture in complex treatment of trophic ulcers of venous etiology in 23 patients with trophic ulcers of lower extremities. The cause of the appearance of ulcers was postthrombophlebitic disease in 16 patients and varicose disease in 7 patients. A control group consisted of 25 patients (postthrombophlebitic disease in 18 patients and varicose disease in 7 patients).

Ointments with superoxide dismutase and interleukin-1beta: effect on reparative processes and impedance of burn wound.

We found that tissue impedance can serve as a reliable criterion of the severity of wound process and efficiency of burn treatment. Ointment with superoxide dismutase effectively promoted wound reparation and recovery of tissue structure after thermal skin burn compared to ointments containing interleukin-1beta or mixture of interleukin-1beta and superoxide dismutase.

Effects of new wound dressings on healing of thermal burns of the skin in acute radiation disease.

Effects of new wound dressing bacterial cellulose impregnated with SOD and poviargol (Procel-Super and Procel-PA) and Inerpan hydrogel dressing on the reparative processes in deep dermal burns (IIIa-IIIb degree) in rats exposed to total even irradiation in a dose of 4 Gy were studied. Inerpan and Procel-Super dressings proved to be the most effective under these conditions: they accelerated healing of burn wounds by 17.0 and 5.

[UHF-dielectrometry in the assessment of the structural organization of salt solutions and interstitial fluids in normal and cicatricial tissues].

The complex dielectric permittivity of salt solutions with positive and negative salvation as well as healthy and cicatricially changed human skin in situ at the frequencies of 42 and 56.6 GHz was measured. The relation between the dielectric characteristics of water and diluted salt solutions and changes in their structural organization conditioned by different temperatures of samples and the type of salvation of electrolytes was studied.

[Modern principles of the pathogenetic therapy of the burn shock].

The article is devoted to the review of main directions of anti-shock therapy improvement for patients with severe burns. It is shown that the treatment efficiency can be considerably increased both by optimization of infusion volume and by the use of modern infusion drugs with anti-hypoxic and anti-oxidant effects. The use of anti-hypoxic and anti-oxidant drugs permits to change the burn shock course and to decrease its duration.

Effect of Erysod (erythrocyte superoxide dismutase) on blood concentration of reactive oxygen species in patients with severe burns and burn shock.

The dynamics of blood concentrations of reactive oxygen species and LPO products in patients with thermal injuries of different severity was studied. Monitoring of these parameters by chemiluminescent and spectrophotometric techniques helps to predict the course of burn shock and prevent complications. Erysod (0.

[Matrix metalloproteinases MMP-2 and MMP-9 of wound and burn exudates and their effect on extracellular matrix proteins].

The dynamics of matrix metalloproteinases (MMP), as well as of fibronectin concentration in wound and burn fluids was traced. The wound fluid proteolytic activity was studied by gelatin zymography method. The data on degradation of fibronectin and various laminin isoforms by wound fluid proteases show that laminin-1, laminin-2/4 and fibronectin were degraded by wound fluid into small fragments.

[Effect of burn exudate on functional activity of Balb/3T3 cell line and the role of matrix metalloproteinases in this process].

The influence of burn fluid and its matrix metalloproteinases (MMPs) on Balb/3T3 cells was studied. The influence of burn fluid was assessed by morphology and specific functional activities of cells characteristic of the healing process--proliferation, monolayer contraction and migration of cells in wound model. The presence of burn fluid in cultivating medium accelerated cell proliferation by 2.

Erysod, erythrocyte superoxide dismutase preparation: effects on LPO processes and morphological changes in the viscera of rats with burns under conditions of delayed antishock infusion therapy.

Biochemical and morphological changes in the kidneys, liver, heart, and lungs were studied in rats with deep burns of 20% body surface. Erysod (0.47 mg/kg/day) added to antishock therapy notably reduced the intensity of LPO processes in tissues both in cases when infusion therapy was started immediately (by 8-20% 12 h and by 5-24% 24 h after the injury) and when this therapy was 6 h delayed (by up about 36% after 12 h).

[Study of water exchange in skin tissues by microwave dielectrometry].

The complex dielectric permitivity of human skin was measured at a frequency of 42 GHz, and a method for calculating water content in skin tissues in vivo was proposed. The water content reaction of skin to standard physical exercises and nontoxic doses of nicotine was investigated. The functionally related changes in skin dielectric properties were interpreted as structural rearrangements of water contained in blood and tissues fluids.

[Impact of high-energy laser irradiation on pulmonic fibroblast in human embryo].

This impact was attained by medicinal laser application to alexandrite, dye, erbium and gallium arsenid in various conditions on cultures of pulmonic fibroblasts of human embryo. Obtained results were estimated by transmission and scanning microscopy. The highest cell destruction was observed in dye and alexandrite, being less expressed in gallium arsenid under laser irradiation.