Publications by authors named "Pappalardo G"

In 14 healthy volunteers (8 M and 6 F), aged 19 to 33 years, serum and salivary concentrations of ofloxacin, administered in a single oral dose of 300 mg, were measured by high performance liquid chromatography (HPLC) and the microbiological agar diffusion method. The serum peak was observed at hour 1 (2.61 +/- 0.

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For the sterility testing of intravenous solutions, the Pharmacopeia recommend the use of liquid media. However, contamination by a single micro-organism during the test may lead to the inappropriate blocking of the release of the batch. An alternative method using solid media for culturing the membranes used in the filtration was therefore sought.

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Very low concentrations of somatostatin (S-14) strongly potentiate the in vitro aggregation induced by collagen, ristocetin and arachidonic acid, but not that induced by ADP or epinephrine, in both human platelet rich plasmas and gel-filtered platelet preparations. Desensitization phenomena may be induced either by repeated addition of S-14 or long lasting contact between S-14 and platelets.

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The purpose of the present study was to pre- and postoperatively evaluate the anal sphincter after coloanal anastomosis in 20 patients with carcinoma of the rectum at 5.5 to 8 cm from the anal verge. The 20 patients matched age and sex with the controlled subjects.

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Japanese authors have described a procedure for the sex determination of human hair roots without epithelial sheath, paraffin embedded and fluorescent stained for cortical cell nuclei Y or X sex chromatins. This method however is not sufficiently practical for forensic science purposes: it is difficult and not guaranteed to obtain suitable longitudinal hair sections with possible loss of evidence particularly serious when the questioned hair is only one. The authors, researching Y-bodies with Quinacrine, present an alternative technique of preparation of specimens without embedding which is simple and quick, reproducible and satisfying in results and that avoids loss of material.

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An experimental study on rabbit sclerosing cholangitis (SC) secondary to direct injection into the biliary tract of the chemical substances commonly employed for intraoperative sterilization of the hydatid cyst content is reported. Among the various substances utilized, 10% formalin and 30% hypertonic solution caused gross and microscopic lesions compatible with less advanced forms of sclerosing cholangitis. The authors present their hypothesis on the development of SC in operated hydatid cysts of the liver.

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The one-stage intracolonic bypass procedure prevents gastrointestinal secretions and fecal content from coming in contact with an anastomotic site without interrupting the intraluminal continuity of the fecal flow from proximal to distal colon. This can be achieved by the intraluminal implantation of a soft, pliable tube above the anastomotic site. Previous clinical and experimental data have indicated that the intracolonic bypass procedure can protect an anastomosis in the presence of maximal colonic loading, gross dehiscences, or fecal peritonitis.

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The prevalence of protein-calorie malnutrition (PCM) and micronutrient deficiency (MND) at the time of admission and discharge from hospital was evaluated in 91 patients consecutively admitted to the Surgery Clinic of the University Hospital of Rome. Anthropometric parameters and circulating levels of vitamins (A, E, C, B1, and B2) and visceral proteins [retinol-binding protein (RBP, prealbumin (PA), and transferrin] were measured. Twenty-one patients (23%) had moderate to severe PCM, and 70 patients (77%) had normal nutritional status (NNS) at admission.

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The experience with 28 patients affected by hepatic and abdominal hydatidosis and treated by combined medical and surgical therapy is reported. All the patients were subject to preoperative and postoperative medical therapy with mebendazole (50 mg/kg/daily). The percentage of living scolices in hydatid fluid obtained during surgery, evaluated microscopically and by mice inoculation, was lower in patients treated medically than in patients operated on without pre and postoperative medical therapy.

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Bassini's technique represents a fundamental step in the treatment of inguinal hernia. It can be asserted that a new era in its surgical therapy started with Bassini. As many difficulties exist in comprehending the archaic language used a century ago by the Italian surgeon, the principles of his technique are analysed.

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Dakin's solution and a chloroxydizer in a hypertonic sodium chloride solution were tested for bacteriological and clinical effectiveness and stability. No real difference in effectiveness was observed between the two products; however, only the chloroxydizer showed satisfactory stability at 150 days under the test storage conditions.

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Forty-six patients operated on for chronic pancreatitis are reported. 3 (6.4%) underwent splanchnicectomy, 29 (59.

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Fifty patients (27 females, 23 males) operated on for parotid neoplasms are reported. Eleven patients (22%) had benign tumors, 31 (62%) mixed tumors and 8 (16%) malignant tumors. Among patients with benign neoplasms, 10 (90,9%) were treated by enucleation (En.

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The following report includes a description of the technical details of a new surgical method for the total removal of the rectum while preserving the anal sphincter function. Seventeen patients including 11 patients with rectal carcinoma, two with diffuse polyposis involving the rectum, three with ulcerative colitis, and one with Crohn's disease of the colon and rectum have undergone operation by this method in the last 4 years. Clinical follow-up (mean 22 months) of these patients has demonstrated normal and continence for feces and gas.

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