Case of Video-Assisted Thoracoplasty Application in Pulmonary Tuberculosis Treatment.

A patient with extensive fibrocavitary pulmonary tuberculosis and extensively drug-resistant Mycobacterium tuberculosis underwent bilateral video-assisted thoracic surgery thoracoplasty. An examination 4 years post-surgery revealed that the patient was in a satisfactory condition and able-bodied. We performed surgery according to our proposed video-assisted thoracic surgery thoracoplasty technique.

Randomized DNA libraries construction tool: a new 3-bp 'frequent cutter' TthHB27I/sinefungin endonuclease with chemically-induced specificity.

Background: Acoustic or hydrodynamic shearing, sonication and enzymatic digestion are used to fragment DNA. However, these methods have several disadvantages, such as DNA damage, difficulties in fragmentation control, irreproducibility and under-representation of some DNA segments. The DNA fragmentation tool would be a gentle enzymatic method, offering cleavage frequency high enough to eliminate DNA fragments distribution bias and allow for easy control of partial digests.

Thermostable proteins bioprocesses: The activity of restriction endonuclease-methyltransferase from Thermus thermophilus (RM.TthHB27I) cloned in Escherichia coli is critically affected by the codon composition of the synthetic gene.

Obtaining thermostable enzymes (thermozymes) is an important aspect of biotechnology. As thermophiles have adapted their genomes to high temperatures, their cloned genes' expression in mesophiles is problematic. This is mainly due to their high GC content, which leads to the formation of unfavorable secondary mRNA structures and codon usage in Escherichia coli (E.

[Personal e-cards for military personnel and military-medical information system].

The article presents main directions of activities of the medical service, dealing with implementation of personal electronic cards for military personnel, organizing the process of automation of medical service management, military and medical organizations and health care departments. The given article, reveals the on-going activity, concerning creation of the military-medical information system, which will unite all medical units, organizations, and governments into one information space.

Two-stage gene assembly/cloning of a member of the TspDTI subfamily of bifunctional restriction endonucleases, TthHB27I.

The Thermus sp. family of bifunctional type IIS/IIG/IIC restriction endonucleases (REase)-methyltransferases (MTase) comprises thermo-stable TaqII, TspGWI, TspDTI, TsoI, Tth111II/TthHB27I enzymes as well as a number of putative enzymes/open reading frames (ORFs). All of the family members share properties including a large protein size (ca.

Increased levels of antibodies against heat shock proteins in stroke patients.

Ischemic stroke is the second leading cause of death worldwide. One of the main risk factors of the ischemic stroke is atherosclerosis which is a chronic inflammatory and immune-mediated disease. Bacterial infections generate specific human antibodies against various antigens, including Hsps.

[Participation of base hospital in regional trauma system].

The authors demonstrated the ability effective work of base hospital in a regional trauma system as a specialized patient care clinic with polytrauma. An experience of the hospital in the treatment of combat injuries, using scales of objective assessment of the severity of injury was applied.

[Clinicomorphological rationale for mediastinal lymphadenectomy in the surgical treatment of disseminated destructive pulmonary tuberculosis].

The paper analyzes morphological changes and results of surgical treatment in 515 patients with destructive pulmonary tuberculosis. Two hundred and seventy-four patients underwent mediastinal lymphadenectomy and in 241 patients lung resections or pneumonectomies were not followed by lymphadenectomy or solitary caseous molten lymph nodes were removed during an operation. In disseminated destructive pulmonary tuberculosis, active tuberculosis of intrathoracic lymph nodes (ILN) was found in 97%.

[Efficiency of partial pneumonectomies in patients with multidrug-resistant tuberculosis].

The immediate results of partial resections were analyzed in 120 patients with drug-resistant pulmonary tuberculosis, among whom 70 patients had multidrug resistance. A complete clinical effect (abacillation and no decay cavities) was achieved in 117 (97.5%) patients, including in 67 (95.

[Pleural biopsy in the diagnosis of pleurisy: potentialities and efficiency].

The results of pleural biopsy were analyzed in 293 patients with exudative pleurisy, among whom a group of free pleurisy included 88 cases, encapsulated pleurisy and pleural obliteration were observed in 156 and 11 patients, respectively. Thoracoscopy with visual pinching pleural biopsy was performed in 59 patients puncture pleural biopsy was made in 234 patients. There is evidence for that thoracoscopic pleural biopsy had a significant advantage in pleurisy in the phase of organization.