Temporal recording of mammalian development and precancer.

Temporal ordering of cellular events offers fundamental insights into biological phenomena. Although this is traditionally achieved through continuous direct observations, an alternative solution leverages irreversible genetic changes, such as naturally occurring mutations, to create indelible marks that enables retrospective temporal ordering. Using a multipurpose, single-cell CRISPR platform, we developed a molecular clock approach to record the timing of cellular events and clonality in vivo, with incorporation of cell state and lineage information.

Finding a secure base: Exploring children's attachment behaviors with professional caregivers during the first months of daycare.

Recent decades have seen a major rise in demand for daycare services for children aged 0 to 3 years, and this has increased research interest in the child-professional caregiver relationship at daycare centers: How does the relationship between children and their new caregivers develop over time? How long does it take for children to settle in at daycare? What variables can influence the settling-in process? These questions are all of the utmost salience and bear crucial implications for children, parents, and daycare practitioners. In this study, we set out to explore the relationship between infants and their new caregivers over the first two months in daycare, using the Professional Caregiver Attachment Diary. The study involved seven Italian daycare centres and 55 professional caregivers, who observed 148 children (M=17.

Temporal recording of mammalian development and precancer.

Key to understanding many biological phenomena is knowing the temporal ordering of cellular events, which often require continuous direct observations [1, 2]. An alternative solution involves the utilization of irreversible genetic changes, such as naturally occurring mutations, to create indelible markers that enables retrospective temporal ordering [3-8]. Using NSC-seq, a newly designed and validated multi-purpose single-cell CRISPR platform, we developed a molecular clock approach to record the timing of cellular events and clonality , while incorporating assigned cell state and lineage information.

Parent, child, and environmental predictors of vegetable consumption in Italian, Polish, and British preschoolers.

This study compared the vegetable intake of preschool children from three European countries [Italy, Poland, and the United Kingdom (UK)] and explored the parent, child, and environmental factors that predicted intake in each country. A total of 408 parents of preschoolers (Italy: = 61, Poland: = 124, and UK: = 225; child mean age = 32.2 months, = 9.

Theory of Mind Development in Italian Children with Specific Language Impairment and Autism Spectrum Disorder: Delay, Deficit, or Neither?

Some studies report delayed theory of mind (ToM) development in children with specific language impairment (SLI), while others do not. A ToM delay is acknowledged in children with autism spectrum disorder (ASD), while whether these children also display a deficit is still under debate. In the current study, we drew on a developmental trajectory approach to assess whether children with SLI or ASD displayed delays or deficits in their ToM performance.

Corrigendum: See & Eat! Using E-books to Promote Vegetable Eating Among Preschoolers: Findings From an Italian Sample.

[This corrects the article DOI: 10.3389/fpsyg.2021.

See & Eat! Using E-books to Promote Vegetable Eating Among Preschoolers: Findings From an Italian Sample.

Different strategies have been developed to help parents with introducing new or disliked vegetables. Nonetheless, many parents of preschoolers struggle against children's refusal to eat vegetables. In this study, we aimed to evaluate the effectiveness of e-books in promoting positive attitudes toward vegetables through repeated visual exposures.

Radiolytic Degradation of Soil Carbon from the Mojave Desert by Co Gamma Rays: Implications for the Survival of Martian Organic Compounds Due to Cosmic Radiation.

The martian surface has been continuously exposed to galactic cosmic radiation. Since organic compounds are degraded by ionizing radiation, knowledge of their decay constants is fundamental to predicting their stability on the martian surface. In this study, we report the radiolysis constant for the destruction of soil organic compounds at a starting concentration of ∼2011 μg C/g from the Mojave Desert.

Production of nitrates and perchlorates by laser ablation of sodium chloride in simulated Martian atmospheres. Implications for their formation by electric discharges in dust devils.

Nitrates and perchlorates are present both on Earth and Mars. In the Martian environment perchlorates dominate over nitrates whereas on Earth is contrariwise. This implies that the mechanisms responsible for their formation are different for both planets.

Fndc-1 contributes to paternal mitochondria elimination in C. elegans.

Paternal mitochondria are eliminated following fertilization by selective autophagy, but the mechanisms that restrict this process to sperm-derived organelles are not well understood. FUNDC1 (FUN14 domain containing 1) is a mammalian mitophagy receptor expressed on the mitochondrial outer membrane that contributes to mitochondrial quality control following hypoxic stress. Like FUNDC1, the C.

Ubiquitination is required for the initial removal of paternal organelles in C. elegans.

Elimination of paternal mitochondria after fertilization occurs in many species using the process of selective autophagy. The mechanism for targeting paternal mitochondria, but not maternal mitochondria, for elimination in the early embryo is not well understood. The results in this paper suggest that there are at least two different mechanisms for targeting paternal mitochondria for elimination: the first involving ubiquitination and a second involving a mitochondrial associated autophagy receptor, fndc-1.

Theory of Mind Development in Children with Visual Impairment: The Contribution of the Adapted Comprehensive Test ToM Storybooks.

Research that focused on Theory of Mind (ToM) development in blind children showed that they were delayed, but not permanently deficient, in various types of false belief tasks. More recent studies reported first evidence of typical ToM development in blind children and suggested that more comprehensive tools to evaluate ToM had to be used. The current paper analyzed ToM development in blind children, using the adapted version of the ToM Storybooks; this is a standardized comprehensive test developed to provide a reliable and stable measurement, in comparison with the false belief tasks.

Children With Cerebral Palsy Playing With Mainstream Robotic Toys: Playfulness and Environmental Supportiveness.

Play is a right for every child and has a key role in child development. Play can be analyzed according to the construct of playfulness, which is the child's disposition to play. Children with cerebral palsy (CP) show difficulties in play and can also experience lower playfulness scores when compared to matched typically developing children.

The Factor Structure of the CA-MIR as Evaluated Using Confirmatory Factor Analysis.

The aim of the current study was to evaluate the factor structure of the CArtes- Modèles Individuels de Relations (CA-MIR), a self-report questionnaire designed to tap into the relational strategies of adults that was developed by a French-speaking research group coordinated by Blaise Pierrehumbert. The CA-MIR's particular merit lies in the richness and complexity of the theoretical model underpinning it. However, to date, this model has only been partially reproduced in studies using exploratory analysis and has never been tested via confirmatory factor analysis (CFA).

The Migrant Border Crossing Study: A methodological overview of research along the Sonora-Arizona border.

Increased border enforcement efforts have redistributed unauthorized Mexican migration to the United States (US) away from traditional points of crossing, such as San Diego and El Paso, and into more remote areas along the US-Mexico border, including southern Arizona. Yet relatively little quantitative scholarly work exists examining Mexican migrants' crossing, apprehension, and repatriation experiences in southern Arizona. We contend that if scholars truly want to understand the experiences of unauthorized migrants in transit, such migrants should be interviewed either at the border after being removed from the US, or during their trajectories across the border, or both.

Social Cognition in Preschoolers: Effects of Early Experience and Individual Differences.

Social cognition is the way in which people process, remember, and use information in social contexts to explain and predict their own behavior and that of others. Children's social cognition may be influenced by multiple factors, both external and internal to the child. In the current study, two aspects of social cognition were examined: Theory of Mind and Emotion Understanding.

Factorial structure of the 'ToM Storybooks': A test evaluating multiple components of Theory of Mind.

This study examined the factorial structure of the Theory of Mind (ToM) Storybooks, a comprehensive 93-item instrument tapping the five components in Wellman's model of ToM (emotion recognition, understanding of desire and beliefs, ability to distinguish between physical and mental entities, and awareness of the link between perception and knowledge). A sample of 681 three- to eight-year-old Italian children was divided into three age groups to assess whether factorial structure varied across different age ranges. Partial credit model analysis was applied to the data, leading to the empirical identification of 23 composite variables aggregating the ToM Storybooks items.

Emotion regulation strategies in preschool children.

This study investigated the development of emotion regulation strategies as reflected in the narratives of children between the ages of 3 and 6 years. An experimental procedure based on story completion tasks was devised to elicit the emotion-related narratives of 69 preschool children. Coding of the narratives led to the observation of different emotion regulation strategies: Behavioural strategies, social support, and cognitive reappraisal.

The limitations on organic detection in Mars-like soils by thermal volatilization-gas chromatography-MS and their implications for the Viking results.

The failure of Viking Lander thermal volatilization (TV) (without or with thermal degradation)-gas chromatography (GC)-MS experiments to detect organics suggests chemical rather than biological interpretations for the reactivity of the martian soil. Here, we report that TV-GC-MS may be blind to low levels of organics on Mars. A comparison between TV-GC-MS and total organics has been conducted for a variety of Mars analog soils.

Mars-like soils in the Atacama Desert, Chile, and the dry limit of microbial life.

The Viking missions showed the martian soil to be lifeless and depleted in organic material and indicated the presence of one or more reactive oxidants. Here we report the presence of Mars-like soils in the extreme arid region of the Atacama Desert. Samples from this region had organic species only at trace levels and extremely low levels of culturable bacteria.