Radiation-induced bystander effect in healthy G(o) human lymphocytes: biological and clinical significance.

To study the bystander effects, G(0) human peripheral blood lymphocytes were X-irradiated with 0.1, 0.5 and 3 Gy.

Relationship between spontaneous or radiation-induced apoptosis and telomere shortening in G(0) human lymphocytes.

To examine the correlation between spontaneous or radiation-induced apoptosis and telomere shortening, G(0) human peripheral blood lymphocytes were irradiated with X-rays and analyzed for viability, apoptosis, and telomere length. Part of the lymphocytes was kept under liquid-holding conditions for 48 h, and then loaded onto Ficoll-Paque medium to separate apoptotic (high-density) from normal (normal-density) cells. Then all samples were examined for the same three end-points.

Effect of storage conditions of blood on radiation-induced chromosomal aberrations and apoptosis in human lymphocytes.

To evaluate the effect of storage conditions of blood on the direct relationship between radiation-induced chromosome aberrations and apoptosis in human peripheral blood lymphocytes, whole blood was irradiated with 3Gy X-rays. Directly after irradiation, a sample of blood was analyzed for chromosome damage and proliferation index, after phytohaemagglutinin stimulation and incubation at 37 degrees C for 56h. Blood samples were stored for 48h at 4 and 20 degrees C with or without phytohaemagglutinin and analyzed for cell viability and apoptosis at 0, 24 and 48h storage time.

Effects of storage conditions of human whole blood on the viability of lymphocytes.

Purpose: To investigate the optimal storage conditions of human whole blood to retain viability of lymphocytes.

Material And Methods: Phytohaemagglutinin (PHA) stimulated and unstimulated human whole blood samples were stored for 48-96 h up to one week at 4 degrees C, 20 degrees C and 37 degrees C and were analysed for apoptosis. After 96 h and one week of storage unstimulated cultures were stimulated to proliferate.