Anaerobic fungi (AF) efficiently degrade lignocellulosic biomass with unique pseudoroot system and enzymatic properties that can remove polysaccharides and some lignified components from plant cell walls, further releasing acetate, lactate, ethanol, hydrogen (H), etc. As research on AF for bioengineering has become a hot topic, a review of lignocellulosic conversion with AF for methane (CH) and H production is needed. Efficient degradation of lignocellulose with AF mainly relies on multiple free carbohydrate-active enzymes and cellulosomes in the free and bound state.
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