Convenient, high-efficiency multiplex genome editing in autotetraploid alfalfa using endogenous promoters and visual reporters.

Unlabelled: CRISPR/Cas is a simple, robust, versatile tool for plant biology studies and precision plant breeding. However, establishing a high-efficiency gene editing system for multiplex editing of the autotetraploid crop alfalfa ( L.), the most important forage legume worldwide, remains a formidable challenge.