Optimization of Lipid Nanoparticles with Robust Efficiency for the Delivery of Protein Therapeutics to Augment Cancer Immunotherapy.

Lipid nanoparticles (LNPs) have been successful in delivering nucleic acids like siRNA and mRNA, but face challenges in protein delivery due to limited protein encapsulation and endosome escape. In this study, a family of LNPs is developed with robust high efficiency in addressing the multiple barriers in cytosolic protein delivery by incorporating clinically approved ionizable lipids into traditional cationic LNPs. The combination of cationic and ionizable lipids enables efficient protein binding and endosomal escape.

A Dynamic Deferoxamine Polymer with Exceptional Performance in Mitochondrial Iron Depletion and Cytosolic Protein Delivery.

Deferoxamine (DFO) is an FDA-approved naturally occurring iron chelator commonly used to treat transfusion-induced iron overload. The abundant and flexible hydroxamic acid groups in DFO enable exceptional iron binding capacity and high protein binding via hydrogen bonding interactions. However, the applications of DFO to sequester intracellular iron and to deliver proteins inside cells are limited due to poor membrane-permeability.

A Serum Resistant Polymer with Exceptional Endosomal Escape and mRNA Delivery Efficacy for CRISPR Gene Therapy.

Nanoparticle-based mRNA delivery offers a versatile platform for innovative therapies. However, most of the current delivery systems are limited by poor serum tolerance, suboptimal endosomal escape and mRNA delivery efficacy. Herein, a highly efficient mRNA-delivering material is identified from a library of fluoropolymers.

and expression and association with the prognosis in esophageal squamous cell carcinoma.

We investigated whether and were related to esophageal squamous cell carcinoma (ESCC). ESCC microarray datasets and reverse transcriptase qualitative PCR were used to analyze and expression. The GSE120356 and GSE33810 datasets identified and as the candidates and and expression was downregulated in ESCC.

Decreased expression of SPINT1-AS1 and SPINT1 mRNA might be independent unfavorable prognostic indicators in esophageal squamous cell carcinoma.

The serine peptidase inhibitor, Kunitz type 1 antisense RNA1 (), a long non-coding RNA , has been linked to cancer progression. In this study, we aimed to explore the expression in matched esophageal squamous cell carcinoma (ESCC) and normal tissues, and analyze the potential correlations of expression with clinicopathological characteristics, in order to evaluate its prognosis and therapeutic value. SPINT1-AS1 expression was detected in 99 cases of matched ESCC and normal tissues samples using the quantitative real-time polymerase chain reaction method.