Extreme effects of Seabuckthorn extracts on influenza viruses and human cancer cells and correlation between flavonol glycosides and biological activities of extracts.

Seabuckthorn is a medicinal plant that is used to prevent cold. It was tested for its metabolic content followed by activity against cancer and virus. The metabolic distribution of different polarity solvent extractions from the leaves was analyzed by LC-MS/MS.

Berberine-piperazine conjugates as potent influenza neuraminidase blocker.

In these studies, we analyzed substituted piperazine based berberine analogs conjugated through a pentyloxy side chain for their in vitro and in silico biological effects. All the final analogs were screened for their in vitro antiviral action against a collection of different influenza virus strains using the CPE assay and SRB assay. Moreover, their cytotoxicity towards non-cancer cell lines was examined employing Madin-Darby canine kidney (MDCK) cell lines.

A rich source of potential bioactive compounds with anticancer activities by Catharanthus roseus cambium meristematic stem cell cultures.

Ethnopharmacological Importance: Catharanthus roseus (L.) G. Don.

Discovery of berberine based derivatives as anti-influenza agent through blocking of neuraminidase.

In this study, we investigated the antiviral activity of newly synthesized berberine derivatives (BD) against influenza virus infection using several strains in in vitro and in silico. The CPE reduction, pre-incubation, NA activity inhibition and molecular docking assays were used for antiviral evaluation. The anti-influenza activities of BDs were stronger than plant-derived pure commercial berberine, and some of the BDs were more potent than control drug Oseltamivir.

Renal-protective and ameliorating impacts of omega-3 fatty acids against aspartame damaged MDCK cells.

Aspartame is widely used artificial sweeteners as food additives. Several researchers have pointed that the controversial report on the use of aspartame over more than decades. Omega-3 fatty acids are essential and unsaturated fatty acids, and it plays a remarkable role in vision, intelligence, neural development, and metabolism of neurotransmitters.

Synthesis and Evaluation of Antioxidant and Cytotoxicity of the N-Mannich Base of Berberine Bearing Benzothiazole Moieties.

Background: Berberine, a quaternary ammonium salt from the protoberberine group of benzylisoquinoline alkaloids has drawn high attention for its several biological potencies.

Objective: To furnish new rationalized derivatives based on berberine core which can deliver promising antioxidant and cytotoxic activities.

Method: The N-Mannich base of an isoquinoline alkaloid, berberine, bearing substituted benzothiazole moieties was obtained.

Cytotoxic effect of TDZ on human cervical cancer cells.

The present study investigates the anticancer activity of Thidiazuron (TDZ). Anticancer activity of TDZ was evaluated in cervical carcinoma cells (HeLa cells). Sulforhodamine-B (SRB) assay indicates that TDZ was about 100 times more toxic to the cancer cell than normal cells.

β-Alanine intercede metabolic recovery for amelioration of human cervical and renal tumors.

β-Alanine is a non-essential amino acid and presents as a major component of various sports supplements. It is a non-proteogenic amino acid, formed in vivo by degradation of carnosine, anserine, balenine, and dihydrouracil. The present study was aimed at investigating the anti-tumor effects of β-alanine in renal and cervical tumor cells.

Inhibitory effect of 2,4-dichlorophenoxyacetic acid on ROS, autophagy formation, and mRNA replication for influenza virus infection.

Influenza virus has had a high rate of antigenic shift and drift that causes significant morbidity and mortality in humans and animals. The lack of excellent pharmacological treatment underlines the importance of the development of the novel antiviral drugs. We investigated the anti-influenza A and B viruses of 2,4-dichlorophenoxyacetic acid (2,4-D), which is the synthetic analog to auxin and is used as a popular herbicide in the agricultural practices.

Toxicity and efficacy of CdO nanostructures on the MDCK and Caki-2 cells.

This article reports the toxicological effects of synthesized cadmium oxide (CdO) nanostructures via a simple green route using a Polygala tenuifolia root extract on normal and renal tumor cells. First, the formation of cadmium oxide nanostructures were confirmed structurally by Fourier transform infrared spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy (XPS). The powder was crystallized in a cubic structure with a space group of Fm-3m.

Efficacy of carnosine on activation of caspase 3 and human renal carcinoma cell inhibition.

Carnosine is a natural antioxidant dipeptide that is highly concentrated in muscles and brain. The present study investigated the effect of carnosine cell growth inhibition and activation of the caspase-3 enzyme under cell co-culture system. Renal carcinoma and normal cells were co-cultured to provide three-dimensional views for the experimental analyses.

Probing the impact of quercetin-7-O-glucoside on influenza virus replication influence.

Background: Influenza virus is still at large and seriously affects social welfare and health. Dianthus superbus is a well-known medicinal plant widely used in Mongolian and Chinese traditional medicine for anti-inflammatory purposes.

Purpose: To investigate the influence of this novel herbal medicinal product over virus infection and virus-induced symptoms

Method: Quercetin-7-O-glucoside was isolated by bioassay (anti-influenza)-guided fractionation.

Investigation of role of aspartame on apoptosis process in HeLa cells -->.

Aspartame is an artificial sweetener used as an alternate for sugar in several foods and beverages. The study reports that consumption of aspartame containing product could lead to cancer. However, the effect of aspartame on apoptosis process in cancer is not yet understood clearly.

Therapeutic efficacy of natural dipeptide carnosine against human cervical carcinoma cells.

Natural substances have been attracted several researchers in the recent years, because of its potential antioxidant, anti-inflammatory and anti-cancer properties. We have investigated the effect of carnosine on cell viability, apoptosis, DNA damage, reactive oxygen species (ROS) and caspase 3 enzyme expression in human cervical carcinoma and Madin-Darby Kidney Cells (MDCK) cells. Carnosine inhibited cancer cell growth up to 23%.

Anticancer studies of synthesized ZnO nanoparticles against human cervical carcinoma cells.

A metal oxide nanoparticle has been widely investigated for its potential use in the biomedical application. The present study investigates the cytotoxicity of ZnO nanoparticle in human cervical carcinoma cells. Cell viability was determined, and it showed the possible cytotoxic effect of ZnO nanoparticles.

Anti-Proliferative Effect of Copper Oxide Nanorods Against Human Cervical Carcinoma Cells.

Metal oxide nanoparticles have been widely investigated for its use in the pharmacological field. The present study was aimed to investigate the cytotoxicity of copper oxide nanorods in human cervical carcinoma cells. The effect of copper oxide nanorods on cell viability was determined by sulforhodamine-B (SRB) assay.

In vitro anticancer potential of BaCO3 nanoparticles synthesized via green route.

Green synthesis of nanoparticles is a growing research area because of their potential applications in nanomedicine. Barium carbonate nanoparticles (BaCO3 NPs) were synthesized using an aqueous extract of Mangifera indica seed as a reducing agent. These particles were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM), selected area electron diffraction (SAED), Energy-dispersive-X-ray (EDX) and X-ray photoelectron spectroscopy (XPS) analysis.

Therapeutic potential of cyanobacteria against streptozotocin-induced diabetic rats.

Hypoglycemic effect of cyanobacteria has evaluated in the normal and streptozotocin-induced diabetic male albino rats as a mammalian model. Normal and streptozotocin-diabetic rats were orally administered cyanobacteria for 60 consecutive days, and their blood levels of glucose, insulin, C-peptide, lipid peroxidation, body weight and enzyme changes were determined using standard methods. Cyanobacteria administration reduced blood glucose level, and increased plasma insulin, C-peptide levels and restored the body weight.

Homobrassinolide induced conformational changes in hexokinase: a possible mechanism for its antidiabetic potential.

Hormonal regulation of cell growth and development, tissue morphology, metabolism and physiological function in animals and man is a well-established knowledge domain in modern biological science. The present study was carried out to investigate the structural stability of hexokinase when exposed to diabetic levels of glucose and its binding efficiency. The fluorescence study indicated that 28-homobrassinolide was able to protect or restore the native structure of hexokinase.

ZnO nanoparticles augment ALT, AST, ALP and LDH expressions in C2C12 cells.

The present study aimed to investigate the effect of ZnO nanoparticles on alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) enzyme expressions in C2C12 cells. ZnO nanoparticles are widely used in the several cosmetic lotions and other biomedical products. Several studies report on ZnO nanoparticle mediated cytotoxicity.

ZnO nanoparticles assist the refolding of denatured green fluorescent protein.

Proteins are essential for cellular and biological processes. Proteins are synthesized and fold into the native structure to become active. The inability of a protein molecule to remain in its native conformation is called as protein misfolding, and this is due to several environmental factors.

In Vitro Selective Anti-Proliferative Effect of Zinc Oxide Nanoparticles Against Co-Cultured C2C12 Myoblastoma Cancer and 3T3-L1 Normal Cells.

The zinc oxide (ZnO) nanoparticle has been widely used in biomedical applications and cancer therapy and has been reported to induce a selective cytotoxic effect on cancer cell proliferation. The present study investigated the cytotoxicity of ZnO nanoparticles against co-cultured C2C12 myoblastoma cancer cells and 3T3-L1 adipocytes. Our results showed that the ZnO nanoparticles could be cytotoxic to C2C12 myoblastoma cancer cells than 3T3-L1 cells.

In Vitro Therapeutic Potential of Tio2 Nanoparticles Against Human Cervical Carcinoma Cells.

Cellular and physiological responses to the degradation products of titanium implants are key indicators to determine the quality of biocompatibility of implant devices. The present study investigated titanium dioxide (TiO2) nanoparticle-induced cytotoxicity, apoptotic morphological modification, and apoptotic-related gene expressions in the human cervical carcinoma cells. TiO2 nanoparticle-induced cytotoxicity on cancer cells was determined by the sulphorhodamine-B assay.

Cytotoxic effects of aspartame on human cervical carcinoma cells.

Aspartame is used as an artificial sweetener in more than 6000 food varieties. The present study aims to determine the effects of aspartame at various concentrations on the cell viability, morphology, ROS level and DNA of human cervical carcinoma cells over two time periods of exposure. The effects of aspartame on HeLa cell viability were investigated using the sulphorhodamine-B assay (SRB assay) and flow cytometry.

Time and Concentration-Dependent Therapeutic Potential of Silver Nanoparticles in Cervical Carcinoma Cells.

Silver nanoparticles (AgNPs) have well-known anti-bacterial properties and have been widely used in daily life as various medical and general products. There is limited information available on the cytotoxicity of AgNPs. Therefore, the present study aimed to investigate the cytotoxicity of AgNPs in HeLa cells.