Elucidating the wedelolactone biosynthesis pathway from Eclipta prostrata (L.) L.: a comprehensive analysis integrating de novo comparative transcriptomics, metabolomics, and molecular docking of targeted proteins.

Eclipta prostrata belongs to the Asteraceae family. The plant contains bioactive compounds like wedelolactone (WDL) and demethylwedelolactone (DW). Its transcriptomic information engaged with secondary metabolite biosynthesis is not available.

Extreme temperatures elicit the accumulation of cardiac glycoside and their genin units in Calotropis procera by altering the expression of transcripts involved in its biosynthesis.

Calotropis procera (Aiton) W.T. Aiton is a medicinal plant belonging to the family Apocynaceae as a core source of natural cardenolides.

Changing practices, procedures, and perceptions: A multi-year person-centered planning initiative in group homes for residents with intellectual and developmental disabilities.

Person-centered planning has been shown to benefit people with disabilities and their quality of life. However, we have little knowledge of how person-centered planning can benefit staff and administration within a group home organization, as well as the extent to which it results in changes to organizational practices and procedures, as well as perceptions of people with disabilities. In this qualitative study, we explored the perspectives of organizational employees, an affiliating behavioral consultant, and residents with intellectual and developmental disabilities, taking into consideration key insights from person-centered planning consultant-coaches, to understand the effects of a person-centered planning initiative on the group home organization.

Mixed methods systematic review: Using a cultural validity assessment to evaluate prevention programs for Indigenous students.

Implementing culturally appropriate school-based prevention programs with Indigenous students that leverage culture as a protective factor has the potential to revitalize and sustain cultural connections that have historically and systematically been destroyed in the United States. However, there is a dearth of literature synthesizing the effectiveness of school-based prevention programs that have been implemented with Indigenous students across contexts. As such, we conducted a mixed method systematic review to (a) evaluate school-based prevention programs with quantitative and/or qualitative data, (b) assess the use of Indigenous research methods, and (c) examine cultural and community validity.

The CARES classroom observation tool: Psychometrics of an observational measure of culturally responsive practices.

There is growing interest in improving and assessing teachers' use of culturally responsive practices (CRP) in the classroom, yet relatively few research-based approaches exist to address these measurement gaps. This article presents findings on the psychometric properties of a newly developed classroom observation measure of CRP, called the CARES Observational Assessment Tool, where CARES refers to five theorized domains of CRP. We used a sample of 268 30-min video observations across urban classrooms (Grades 4-9) from the Measures of Effective Teaching Project (Bill and Melinda Gates Foundation, 2014).

Anomalous Thermal Transport in Compressed Carbon Phases.

Carbon materials display intriguing physical properties, including superconductivity and highly anisotropic thermal conductivity found in graphene. Compressive strain can induce structural and bonding transitions in carbon materials and create new carbon phases, but their interplay with thermal conductivity remains largely unexplored. We investigated the in situ high-pressure thermal conductivity of compressed graphitic phases using picosecond transient thermoreflectance and first-principles calculations.

Biophysical significance of fluorescence spectroscopy in deciphering nucleic acid dynamics: From fundamental to recent advancements.

Fluorescence spectroscopy has revolutionized the study of nucleic acids, providing invaluable insights into the dynamic processes that underpin gene expression, replication, and repair. This review explores the application of fluorescence probes in monitoring the conformational changes, interactions, and regulatory mechanisms of DNA and RNA. We discuss the utility of intercalating and non-intercalating fluorescent probes in real-time tracking of nucleic acid dynamics, highlighting their role in elucidating the molecular mechanisms of DNA replication, transcriptional regulation, and DNA repair.

The role of hydrogen sulfide (HS) in postharvest fruits: A comprehensive analysis.

Hydrogen sulfide (HS) has emerged as a crucial signaling molecule in plant biology, exhibiting diverse roles in growth, development, and stress responses. In recent years, its involvement in postharvest physiology has drawn significant attention, particularly in the context of fruit ripening, senescence, and quality maintenance. However, despite the increasing recognition of HS's importance in postharvest processes, there remains a limited understanding of the specific molecular mechanisms by which HS modulates these physiological responses and its interaction with other signaling pathways in fruit tissues.

Musculoskeletal imaging fellowship program directors: A study of educational paths, and scholarly engagement in the United States.

Purpose: The overall aim of the study is to analyze and provide a comprehensive understanding of the demographics, educational backgrounds, and scholarly activities of musculoskeletal imaging fellowship program directors across the United States.

Methods: A list of all members of the Society of Skeletal Radiology was obtained and musculoskeletal imaging fellowship program directors across the US were included. Publicly available online sources were used to gather demographic and educational information about each musculoskeletal imaging fellowship program director, which included the online curriculum vitae from the program websites, Health Grades, Doximity, and Elsevier's Scopus database.

Chitosan-functionalized nanobubbles for precision oncology: advances in targeted cancer therapeutics.

The convergence of nanotechnology and cancer therapeutics has opened new frontiers in the development of advanced drug delivery systems. Among the various nanocarriers, nanobubbles offer significant potential due to their unique properties, such as high payload capacity, responsiveness to external stimuli like ultrasound, and enhanced permeability and retention (EPR) effects. Functionalizing these nanobubbles with chitosan, a naturally derived biopolymer known for its biocompatibility, biodegradability, and ability to enhance cellular uptake, further improves their therapeutic efficacy.

A Research-Practice Partnership to Develop the R-CITY Multi-Component, Equity-Focused Social-Emotional Learning Intervention.

There is growing interest in the integration of social-emotional learning (SEL) and equity approaches in schools, yet systematic research on how to blend these two frameworks is limited. In this article, we describe the process by which a research-practice partnership (RPP) collaborated to iteratively co-create a multi-component equity-focused SEL preventive intervention in the context of a politically charged landscape related to the 'dual pandemics' of racial injustice and COVID-19 in the early 2020s. We conducted a document review of informal data sources (e.

Biophysical characterization of hydrogen sulfide: A fundamental exploration in understanding significance in cell signaling.

Hydrogen sulfide (H₂S) has emerged as a significant signaling molecule involved in various physiological processes, including vasodilation, neurotransmission, and cytoprotection. Its interactions with biomolecules are critical to understand its roles in health and disease. Recent advances in biophysical characterization techniques have shed light on the complex interactions of H₂S with proteins, nucleic acids, and lipids.

ER-GUARD: an evolutionarily conserved antioxidant defense system at ER membranes.

Oxidative protein folding in the endoplasmic reticulum (ER) is essential for all eukaryotic cells yet generates hydrogen peroxide (HO), a reactive oxygen species (ROS). The ER-transmembrane protein that provides reducing equivalents to ER and guards the cytosol for antioxidant defense remains unidentified. Here we combine AlphaFold2-based and functional reporter screens in to identify a previously uncharacterized and evolutionarily conserved protein ERGU-1 that fulfills these roles.

Surgeon Experience as Predictors of Outcome of Dacryocystorhinostomy Surgery.

Background: Dacryocystorhinostomy surgery aims to treat nasolacrimal duct obstruction and are often performed by general ophthalmologists in developing countries. The aim of this study is to estimate the clinical burden, surgeon's experience, and outcome of dacryocystorhinostomy surgery.

Methods: A cross-sectional survey of Nepalese Ophthalmologists registered with the Nepal Ophthalmic Society was conducted after ethical clearance from Nepal Health Research Council.

Understanding the bio-crystallization: An insight to therapeutic relevance.

In the realm of biomedical engineering and materials science, the synthesis of biomaterials plays a pivotal role in advancing therapeutic strategies for regeneration of tissues. The deliberate control of crystallization processes in biomaterial synthesis has emerged as a key avenue for tailoring the properties of these materials, enabling the design of innovative solutions for a wide array of medical applications. This review delves into the interplay between controlled crystallization and biomaterial synthesis, exploring its multifaceted applications in the therapeutic domains.

LPD-3 as a megaprotein brake for aging and insulin-mTOR signaling in C. elegans.

Insulin-mechanistic target of rapamycin (mTOR) signaling drives anabolic growth during organismal development; its late-life dysregulation contributes to aging and limits lifespans. Age-related regulatory mechanisms and functional consequences of insulin-mTOR remain incompletely understood. Here, we identify LPD-3 as a megaprotein that orchestrates the tempo of insulin-mTOR signaling during C.

Scalar invariant transform based deep learning framework for detecting heart failures using ECG signals.

Heart diseases are leading to death across the globe. Exact detection and treatment for heart disease in its early stages could potentially save lives. Electrocardiogram (ECG) is one of the tests that take measures of heartbeat fluctuations.

Hydrogen sulfide (H2S) metabolism: Unraveling cellular regulation, disease implications, and therapeutic prospects for precision medicine.

Hydrogen sulfide (HS), traditionally recognized as a noxious gas with a pungent odor, has emerged as a fascinating metabolite originating from proteinaceous foods. This review provides a comprehensive examination of HS regulatory metabolism in cell. Dysregulation of cellular processes plays a pivotal role in the pathogenesis of numerous diseases.

Characterization, Antioxidant, and Antimicrobial Properties of Mulberry Lattices.

In order to find the most advantageous bioactive compounds from mulberry latex for drug development in the near future, this study was conducted to characterize and evaluate antioxidant and antimicrobial properties from four different mulberry lattices (BR-2, S-1, AR-14, and S-146). The characterization of the lattices was performed by scanning electron microscopy with energy-dispersive X-ray spectroscopy, gas chromatography coupled to mass spectroscopy, and Fourier transform infrared spectroscopy. Further, screenings of the antioxidant and antimicrobial potential of selected lattices were performed in vitro using 2,2-diphenyl-1-picrylhydrazyl assay and agar well diffusion methods, respectively.