Black soldier fly larvae mediate Zinc and Chromium transformation through the ZnuCBA and citric acid cycle system.

Intestinal microbiota and metal regulatory proteins (MRPs) underlie the transformation of heavy metals (HMs) by the black soldier fly larvae (BSFL), but the mechanisms involved are still not fully defined. Here, using 16S rRNA and metagenomics-assisted tracing, we found that zinc (Zn) and chromium (Cr) stress led to enrichment of Proteobacteria in the BSFL intestine. Support of Proteobacteria also led to increased levels of the Zn transporter proteins ZnuC/B/A and the Zn efflux proteins zntR/A.

Research Progress on Douchi Fibrinolytic Enzyme.

In recent years, the morbidity and mortality owing to thrombotic diseases have increased. Fibrinolytic enzymes can dissolve thrombosis. Existing thrombolytic drugs are expensive, have short half-lives, and cause bleeding easily.

Morphological description and DNA barcoding research of nine species.

Introduction: plants are highly valued for their ornamental qualities. However, traditional morphological identification methods are inefficient for discriminating species. DNA barcoding has emerged as a powerful alternative for species identification, but research on DNA barcodes is still limited.

Study on the Regulatory Mechanisms of Carapace Marking Formation in .

There are two phenotypes in the natural populations of , which is an ideal model for studying the formation of markings and body color in crustaceans. In a previous study, we used comparative transcriptome technology to screen some functional genes related to body color regulation. Here, high-throughput sequencing technology was used to perform microRNA (miRNA) sequencing analysis on the exoskeleton of with two types of carapace markings, and functional studies of related genes were performed.

Effectiveness and safety of ceftazidime-avibactam in Chinese patients with hospital-acquired pneumonia, including ventilator-associated pneumonia: a Phase 4, multi-centre, open-label study.

Background: Ceftazidime-avibactam was approved in patients with hospital-acquired pneumonia (HAP), including ventilator-associated pneumonia (VAP), based on the multinational Phase 3 REPROVE study. This study assessed efficacy and safety of ceftazidime-avibactam in Chinese adults with HAP/VAP.

Methods: This was a Phase 4, prospective, multi-centre, open-label study, conducted at 42 sites in China.

Aqueous extract of Rehmanniae Radix Praeparata improves bone health in ovariectomized rats by modulating the miR-29a-3p/NFIA/Wnt signaling pathway axis.

Ethnopharmacological Relevance: Rehmanniae Radix Praeparata (RRP), a widely used traditional Chinese medicine and a processed form of Rehmannia glutinosa, is primarily utilized to supplement kidney function and promote bone health. Clinical evidence suggests that RRP exhibits significant efficacy in the treatment of osteoporosis (OP). However, the precise mechanisms underlying its therapeutic effects remain incompletely understood.

p85β acts as a transcription cofactor and cooperates with BCLAF1 in the nucleus.

p85β is a regulatory subunit of the phosphoinositide 3-kinase (PI3K). Emerging evidence suggests that p85β goes beyond its role in the PI3K and is functional in the nucleus. In this study, we discover that nuclear p85β is enriched at gene loci and regulates gene transcription and that this regulatory role contributes to the oncogenic potential of nuclear p85β.

Simple and low-cost synthesis of g-CN for efficient sunlight photocatalytic degradation of antibiotics in biogas slurry.

Applying biogas slurry containing antibiotics as organic fertilizer to farmland can lead to the accumulation of antibiotics in the soil and access the water environment further through surface runoff and leachate, posing a potential risk to the regional water environment. In this study, photocatalysis was performed to degrade the antibiotics in biogas slurry employing g-CN as a catalyst under visible light and sunlight. g-CN was prepared by simple thermal polymerization at different temperatures and times with cheap urea and melamine, respectively, and the yield, structural characterization, and photocatalytic performance were analyzed.

Low-intensity pulsed ultrasound enhances delivery of 30 nm Q10 for improving mental and memory disorder in APP/PS1 mice.

Patients with Alzheimer's disease (AD) often experience mental and memory disorders with poor outcomes. Coenzyme Q10 can degrade formaldehyde (FA) and improve Alzheimer-related symptoms, but its ability to cross the blood-brain barrier (BBB) is limited. This study investigated whether low-intensity pulsed ultrasound (LIPUS) enhances 30 nm Q10 delivery and improve symptoms in AD model mice.

Associations of urinary essential metals and their mixture with metabolic syndrome and its components among Chinese community-dwelling older adults.

Background: Little is known regarding the joint effect of multiple essential metals (EMs) on metabolic syndrome (MetS). This study aimed to investigate individual and overall correlations of EMs with MetS and its components among Chinese community-dwelling older adults.

Methods: Six urine EMs, including molybdenum (Mo), vanadium (V), selenium (Se), calcium (Ca), cobalt (Co), and magnesium (Mg), were measured using Inductively Coupled Plasma Mass Spectrometry (ICP-MS) in 2222 older adults.

Depressive symptoms changes in the new-onset stroke patients: A cross-lagged panel network analysis.

Background: Each year, there are approximately 10.3 million new stroke cases worldwide, with 2 million occurring in China. Post-stroke depression (PSD) is a common complication that negatively affects rehabilitation outcomes and increases long-term mortality.

Discovery of Cationic Lugdunin Derivatives with Membrane-Disrupting Activity against Resistant Bacteria via Radical Reactions and Amino Acid Mutations.

Lugdunin was the first reported new class of thiazolidine cyclopeptide antibiotic from human microbiomes. However, its structure could not accommodate polar amino acids or modules, limiting its potential for chemical modification and clinical application. Herein, we developed a combinatorial modification strategy based on in situ modification of tryptophan through a radical reaction and amino acid site-specific mutation, transforming lugdunin into a cationic cyclic peptide antibiotic.

Lipid alterations in acute myocardial infarction are associated with gut microbiota.

Although lipid metabolism and gut microbiota dysregulation are known to participate in cardiovascular disease, few studies have examined these alterations and associations in acute myocardial infarction (AMI) patients. This study reveals altered and associated lipid metabolism and gut microbiome based on proteomic and lipidomic analyses of fecal and plasma samples from 30 patients with AMI and 33 healthy volunteers. Twenty-three differential plasma lipids, nine altered fecal lipids, and nine differential fecal bacterial species were identified in AMI patients relative to healthy volunteers, with nine plasma lipids, three fecal lipids, and two fecal bacteria as potential biomarkers.

The cancer-associated fibroblasts interact with malignant T cells in mycosis fungoides and promote the disease progression.

Background: Cutaneous T-cell lymphoma (CTCL) is a heterogeneous group of T-cell lymphomas characterized with the presence of clonal malignant T cells. Mycosis fungoides (MF) is the most common type of CTCL. However, the pathogenesis of MF and the role of the tumor microenvironment (TME) remain unclear.

Marein alleviates the development of atherosclerosis through targeting miR-126/lipoprotein-associated phospholipase A axis.

As a classical precious medicine, Coreopsis tinctoria Nutt. (C. tinctoria) is widely utilized for treatment of cardiometabolic diseases, while the important compound of C.

The chain mediating effects of psychological capital and illness perception on the association between social support and acceptance of illness among Chinese breast cancer patients: A cross-sectional study.

Purpose: Acceptance of illness is crucial for effective breast cancer management and better health outcomes. Although social support is known to aid illness acceptance, the underlying mechanisms remain unclear. This study aims to examine the chain mediating role of psychological capital and illness perception in the relationship between social support and acceptance of illness among Chinese breast cancer patients.

NEIL3 and TOP2A as key drivers of esophageal cancer through WNT signaling.

Esophageal cancer (EC) is a highly aggressive malignancy with limited treatment options. Nei like DNA glycosylase 3 (NEIL3) and DNA topoisomerase II alpha (TOP2A) have been identified as potential therapeutic targets, though their roles in EC remain unclear. This study investigates the effects of NEIL3 overexpression and TOP2A knockdown, focusing on the WNT signaling pathway.

Quality Consistency Evaluation of Chemical Composition and Pharmacology of Pheretima aspergillum Dispensing Granules and Traditional Decoction Based on HPLC, GC-IMS, and Animal Experiments.

This study investigates the consistency of quality between the traditional decoction (TD) of Pheretima aspergillum and its dispensing granule decoction (DGD) by examining their chemical composition and antithrombotic efficacy. We first determined the nucleoside components, protein content, and volatile compounds in 10 batches of TD and 9 batches of DGD sourced from three manufacturers using high-performance liquid chromatography (HPLC), UV-vis spectrophotometry, and gas chromatography ion mobility spectrometry (GC-IMS). Next, we assessed antithrombotic efficacy by measuring the relative length of tail thrombosis and calculating the thrombus inhibition rates at 24 and 48 h.

Association between exposure to environmental pollutants and increased oral health risks, a comprehensive review.

The burden of disease and death attributable to environmental pollution is a growing public health challenge worldwide, particularly in developing countries. While the adverse effects of environmental pollution on oral health have garnered increasing attention, a comprehensive and systematic assessment remains lacking. This article delves into the intricate relationship between environmental pollution and oral health, highlighting significant impacts on various aspects such as dental caries, periodontal diseases, oral facial clefts, cancer, as well as other oral diseases.

Risk of myocardial infarction and heart failure in gout patients: a systematic review and meta-analysis.

Background: Gout is a metabolic disease caused by decreased blood uric acid excretion and purine metabolism disorders. Long-term and persistent metabolic dysfunction gradually affects other organ functions and is the main factor inducing Myocardial Infarction (MI) and Heart Failure (HF), seriously affecting the health of patients. This study adopts a meta-analysis to analyze the risk of MI and HF in gout patients.

Single-cell and bulk RNA sequencing analysis reveals CENPA as a potential biomarker and therapeutic target in cancers.

Background: Cancer remains one of the most significant public health challenges worldwide. A widely recognized hallmark of cancer is the ability to sustain proliferative signaling, which is closely tied to various cell cycle processes. Centromere Protein A (CENPA), a variant of the standard histone H3, is crucial for selective chromosome segregation during the cell cycle.

Technical and Biological Biases in Bulk Transcriptomic Data Mining for Cancer Research.

Cancer research has been significantly advanced by the integration of transcriptomic data through high-throughput sequencing technologies like RNA sequencing (RNA-seq). This paper reviews the transformative impact of transcriptomics on understanding cancer biology, focusing on the use of extensive datasets such as The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx). While transcriptomic data provides crucial insights into gene expression patterns and disease mechanisms, the analysis is fraught with technical and biological biases.

Increased ferritin, serum lactate dehydrogenase, and aspartate aminotransferase levels predict macrophage activation syndrome complicating systemic lupus erythematosus: a retrospective study.

Background: This study aimed to assess the diagnosis of macrophage activation syndrome (MAS) at the onset of active childhood-onset systemic lupus erythematosus (cSLE), which is under-researched, and to compare the characteristics of cSLE with and without MAS, hypothesizing the existence of possible predictors of MAS in active cSLE.

Methods: This study enrolled 157 patients diagnosed with cSLE, with or without MAS, from Nanjing Medical University between January 2018 and May 2023. Data analysis was performed using an independent samples -test or the Mann-Whitney -test, the test, the Youden index to determine the optimal cutoff values for diagnosis, and binary logistic regression analysis to determine the predicted probability.

Neurological Nurses' Perspectives on Communication of Stroke Recurrence Risks: A Mixed-Method Study.

Aim: To gain a comprehensive understanding of the practices, attitudes, and thoughts of neurological nurses regarding communication about the risk of stroke recurrence.

Design: This is a mixed-method study with a sequential explanatory design.

Methods: An electronic survey was conducted amongst 280 neurological nurses from 30 hospitals to explore their clinical practice and attitudes towards stroke recurrence risk communication (RC).

Hybrid Enzyme-Electrocatalyst Cascade Modified Gas-Diffusion Electrodes for Methanol Formation from Carbon Dioxide.

We propose a hybrid electrocatalytic-bioelectrocatalytic reaction cascade integrated on a gas diffusion electrode for CO reduction under selective formation of methanol. Ag-BiO selectively reduces gaseous CO to formate at neutral pH conditions. A subsequent enzymatic cascade comprising formaldehyde dehydrogenase and alcohol dehydrogenase, which are both nicotinamide adenine dinucleotide (NAD)-dependent, further reduce formate sequentially to formaldehyde and methanol.