Cell-Cultured Influenza Vaccine Enhances IFN-γ+ T Cell and Memory T Cell Responses Following A/Victoria/2570/2019 IVR-215 (A/H1N1) Infection.

Background: Influenza remains a significant public health challenge, with vaccination being a substantial way to prevent it. Cell-cultured influenza vaccines have emerged to improve on the drawbacks of egg-based vaccines, but there are few studies focusing on T cell immunity with both types of vaccines. Therefore, we studied the following 2022-2023 seasonal influenza vaccines with a standard dose and high dose: cell-based (C_sd and C_hd) and egg-based (E_sd and E_hd) vaccines.

Highly Transparent Ka-/W-Band Electromagnetic Shielding Films Based on Double-Layered Metal Meshes.

An electromagnetic (EM) wave-shielding film exhibiting high performance in high-frequency bands, such as the Ka- and W-bands, was fabricated by using double-layered metal meshes. The double-layered shielding (DLS) film consists of metallic micromesh and nanomesh electrodes (NMEs) on the upper and lower surfaces of a poly(ethylene terephthalate) (PET) film, respectively. The micromesh electrodes (MMEs) were fabricated such that they possessed a thickness higher than the line width, and they thus exhibited excellent electromagnetic wave-shielding performance in addition to optical transmittance.

Mechanically Stable Flexible Organic Photovoltaics with Silver Nanomesh for Indoor Applications.

Enhanced device performance of flexible organic solar cells (FOSCs) was achieved according to the development of organic solar cells (OSCs). OSCs are promising candidates as energy sources for low-power supply systems such as the Internet of Things (IoT) under indoor lighting environments. To apply FOSCs to flexible or wearable applications, they must be mechanically stable.

A broad-spectrum and highly potent human monoclonal antibody cocktail for rabies prophylaxis.

Post-exposure prophylaxis (PEP) is highly effective in preventing disease progression of rabies when used in timely and appropriate manner. The key treatment for PEP is infiltration of rabies immune globulin (RIG) into lesion site after bite exposure, besides wound care and vaccination. Unfortunately, however, RIG is expensive and its supply is limited.

High-performance flexible transparent nanomesh electrodes.

A cost-effective process for producing high-performance Ag-paste-based flexible transparent nanomesh electrodes (FTNEs) was developed by optimizing their linewidth, pitch, and height. These nanomesh electrodes, with a linewidth of several hundred nanometers and a pitch of 10-200 μm on a PET substrate, achieved wide ranges of transmittance (83.1%-98.

Development and characterization of novel chimeric monoclonal antibodies for broad spectrum neutralization of rabies virus.

Current post-exposure prophylaxis for rabies virus infection has several limitations in terms of supply, cost, safety, and efficacy. Attempts to replace human or equine rabies immune globulins (HRIG or ERIG) have been made by several companies and institutes. We developed potent monoclonal antibodies to neutralize a broad spectrum of rabies viruses by screening hybridomas received from the U.

Hyperglycemia decreases the expression of ATP synthase β subunit and enolase 2 in glomerular epithelial cells.

Glomerular epithelial cells (GECs) are known to play a key role in maintaining the structure and function of the glomerulus. GEC injury induced by hyperglycemia is present in early-stage diabetic nephropathy (DN), which is the most common cause of renal failure. In an attempt to identify target proteins involved in the pathogenesis of GEC injury at early DN, we performed the proteomic analysis using primary cultures of GECs, prepared from the dissected rat glomeruli.

Comparison of tear proteins between healthy and early diabetic retinopathy patients.

Objectives: To identify potential prognostic or diagnostic marker tear proteins for early diabetic retinopathy (DR) and to investigate the pathogenesis of this disease using proteomics techniques.

Design And Methods: The tear proteins expressed in patients suffering from diabetes mellitus without the retinopathy symptoms, nonproliferative diabetic retinopathy and healthy volunteers were analyzed by 2-DE. The differentially expressed proteins in patients were identified by ESI-Q-TOF and confirmed by Western blotting.

Comparative analysis of serum proteomes of patients with cardiovascular disease.

Objectives: To monitor increases or decreases in cardiovascular disease (CVD)-related proteins that will be released from the deposits or plaque on the inner wall of blood vessels.

Design And Methods: Protein profiles of sera from healthy subjects and CVD patients were determined via 2-DE. Differentially expressed spots in CVD patients were identified by ESI-Q-TOF MS/MS.

Differentially expressed proteins of mesenchymal stem cells derived from human cord blood (hUCB) during osteogenic differentiation.

Multipotent mesenchymal stem cells (MSCs) derived from human umbilical cord blood (hUCB) represent promising candidates for the development of future cellular therapy strategies. MSCs have been found to be able to differentiate into various tissues. One of the primary limitations in our understanding of the biology of human MSCs is the absence of prospective markers required for the monitoring of lineage-specific differentiation.

Sample preparation method for plasma membrane proteome analysis.

The preparation of plasma membrane (PM) proteome samples is seriously difficult and time-consuming, owing to their profound hydrophobicity and low abundance. We have developed an efficient PM sample preparation method using Ultracentrifugation with Percoll and an aqueous two-phase extraction. The developed method was rapid (3 h) and provided high purities (26-fold of cell lysate) with a high yield (2.

Proteome analysis of the rat hepatic stellate cells under high concentrations of glucose.

To study the change in hepatic stellate cell (HSC) function under diabetic conditions, we cultured rat HSC in the presence of 5 and 30 mM glucose, which correspond to blood glucose concentrations during the early and late stages of diabetes, respectively. The differentially expressed HSC proteins were analyzed using 2-DE and ESI-Q-TOF MS/MS and confirmed with Western blotting. The changed protein expression will provide greater understanding of glycolysis in HSC at the high concentration of glucose.

The discovery of biomarkers for type 2 diabetic nephropathy by serum proteome analysis.

Diabetic nephropathy (DN) is a serious kidney complication of diabetes, and constitutes the leading cause of end-stage renal disease. The earliest clinical evidence of DN is microalbuminuria, a term which refers to the appearance of small but abnormal amounts of albumin in the urine. However, screening methods for DN, such as biomarker assays, are yet to be developed for type 2 DN.

Proteome analysis of serum from type 2 diabetics with nephropathy.

Diabetic nephropathy (DN) is a renal disease which develops as a consequence of diabetes mellitus. Microalbuminuria is the earliest clinical sign of DN. There are no specific diagnostic biomarkers for type 2 diabetics with nephropathy other than microalbuminuria and macroalbuminuria.