[Ceruloplasmin and peptides of medium molecular weight as parameters in development of acute myeloblastic leukemia].

A dynamic content of ceruloplasmin and medium-molecular peptides were studied in the blood serum of 28 patients with an acute clinical course of myeloblast-type leucosis during a scheduled chemotherapy. The mentioned biochemical criteria were shown to be able to characterize indirectly an intensity of the clinical course, functional liver condition and the efficiency of chemotherapy.

[Levels of extracellular DNA and polyamines as criteria of resistance to chemotherapy in patients with acute leukemia].

Content of polyamines and extracellular DNA were studied in blood of patients with acute leukoses which did not respond to chemotherapy. Concentration of total polyamines was distinctly lower in blood of patients with acute lymphoblast and myeloblast leukoses as compared with healthy persons. Free and total polyamines were unaltered in blood of these patients after cytostatic therapy.

[Middle molecules as an indicator of the degree of disruption of blood coagulation in hemophilia].

The blood plasma levels of medium-mass molecules (MMM) have been studied by UV spectrophotometry in patients with hemophilia A, B, and AB. The hemocoagulation status has been estimated with the use of the parameters reflecting the general blood coagulation ability and with the correction tests, using the thromboplastin generation test. The MMM levels in all hemophilia forms under study have been found essentially elevated vs.

[Determination of DNA level in human plasma].

It has been first demonstrated that DNA concentration in the plasma of healthy donors was 5 to 30 mu kg per I ml of plasma or 10-50% of DNA content in leukocytes contained in the same blood volume. DNA plasma concentration was determined by registering DNA-bisbenzimide complex fluorescence in supernatant derived from plasma containing 10% NaCl upon heating for 2-3 min at 100 degrees C. The method is simple, specific and permits the determination of DNA concentrations in 0.

[Direct fluorimetric determination of the DNA content in human leukocyte homogenates].

A simple and highly sensitive method was suggested for fluorometric determination of DNA content in human blood leukocytes. The method is based on the ability of fluorochrome bisbenzimide to bind specifically to DNA of cell homogenates in the presence of 2.5 M NaCl.

[Content of 5-methylcytosine in DNA fractions in normal human leukocytes and in chronic leukoses].

A procedure is described for fractionation of DNA obtained from leukocyte lysates of healthy donors and of patients with chronic leukoses using silica gel column chromatography. Lymphocytes and granulocytes of healthy donors as well as leukocytes from patients with chronic lympho- and myeloleukosis were dissimilar in the fractional composition of DNA. Nucleotide composition was studied; content of the DNA I fraction varied distinctly both in healthy donor granulocytes and in leukocytes of patients with chronic myeloleukosis as well as in leukocytes of patients with chronic lympho- and myeloleukosis.