The extracellular proteome of two Bifidobacterium species reveals different adaptation strategies to low iron conditions.

Background: Bifidobacteria are among the first anaerobic bacteria colonizing the gut. Bifidobacteria require iron for growth and their iron-sequestration mechanisms are important for their fitness and possibly inhibit enteropathogens. Here we used combined genomic and proteomic analyses to characterize adaptations to low iron conditions of B.

High Iron-Sequestrating Bifidobacteria Inhibit Enteropathogen Growth and Adhesion to Intestinal Epithelial Cells .

The gut microbiota plays an important role in host health, in particular by its barrier effect and competition with exogenous pathogenic bacteria. In the present study, the competition of PV8-2 (Bp PV8-2) and PV20-2 (Bk PV20-2), isolated from anemic infant gut microbiota and selected for their high iron sequestration properties, was investigated against Typhimurium (. Typhi) and O157:H45 (EHEC) by using co-culture tests and assays with intestinal cell lines.

Bifidobacterium pseudolongum Strain PV8-2, Isolated from a Stool Sample of an Anemic Kenyan Infant.

The complete genome sequence of Bifidobacterium pseudolongum PV8-2, isolated from feces of an anemic Kenyan infant, was determined using single-molecule real-time (SMRT) technology. The genome consists of a 2-Mbp chromosome and a 4-kb plasmid.

Complete and Assembled Genome Sequence of Bifidobacterium kashiwanohense PV20-2, Isolated from the Feces of an Anemic Kenyan Infant.

The complete genome sequence of Bifidobacterium kashiwanohense strain PV20-2, an infant feces isolate, was determined using single-molecule real-time sequencing (SMRT). Hierarchical genome assembly resulted in a completely assembled genome of 2,370,978 bp. The B.

Bifidobacteria strains isolated from stools of iron deficient infants can efficiently sequester iron.

Background: Bifidobacteria is one of the major gut commensal groups found in infants. Their colonization is commonly associated with beneficial effects to the host through mechanisms like niche occupation and nutrient competition against pathogenic bacteria. Iron is an essential element necessary for most microorganisms, including bifidobacteria and efficient competition for this micronutrient is linked to proliferation and persistence.

Novel Polyfermentor intestinal model (PolyFermS) for controlled ecological studies: validation and effect of pH.

In vitro gut fermentation modeling offers a useful platform for ecological studies of the intestinal microbiota. In this study we describe a novel Polyfermentor Intestinal Model (PolyFermS) designed to compare the effects of different treatments on the same complex gut microbiota. The model operated in conditions of the proximal colon is composed of a first reactor containing fecal microbiota immobilized in gel beads, and used to continuously inoculate a set of parallel second-stage reactors.