Publications by authors named "Pamela R Menegotto"
Article Synopsis
- NPM1 mutations are found in 20-30% of acute myeloid leukemia (AML) patients, and there are various methods to analyze these mutations, each with its strengths and weaknesses.
- The study compares three nonsequencing methods—multiparameter flow cytometry (MFC), amplification refractory mutation system-PCR (ARMS-PCR), and quantitative PCR (qPCR)—to detect NPM1 mutations and explores nuclear morphometric analysis (NMA) as an alternative for detecting specific blast types.
- Results indicate that qPCR is the most effective method for identifying NPM1 mutations, ARMS-PCR is a more labor-intensive but cheaper option, and MFC serves as a preliminary screening tool that requires further confirmation through
There is an urgent need for advances in the treatment of Ewing sarcoma (EWS), an aggressive childhood tumor with possible neuroectodermal origin. Inhibition of histone deacetylases (HDAC) can revert aberrant epigenetic states and reduce growth in different experimental cancer types. Here, we investigated whether the potent HDAC inhibitor, sodium butyrate (NaB), has the ability to reprogram EWS cells towards a more differentiated state and affect their growth and survival.
View Article and Find Full Text PDFNeonatal handling is an experimental procedure used to analyze the effects of environmental interventions during early postpartum days (PPD). Long-lasting effects of repeated stress exposure in the neonatal period on the maternal side are poorly studied in this model. The aim of this study was to verify if handling the pups induces enduring effects on damśstress responses, increasing their risk for depression.
View Article and Find Full Text PDFGlioblastoma multiforme (GBM) is the most aggressive type of brain tumor, characterized by excessive cell proliferation, resistance to apoptosis, and invasiveness. Due to resistance to currently available treatment options, the prognosis for patients with GBM is very dismal. The activation of gastrin-releasing peptide receptors (GRPR) stimulates GBM cell proliferation, whereas GRPR antagonists induce antiproliferative effects in in vitro and in vivo experimental models of GBM.
View Article and Find Full Text PDFArticle Synopsis
- - The study explored how prenatal stress from maternal restraint affects the social behavior and anxiety of offspring, particularly focusing on specific brain neurons involved in the release of oxytocin (OT) and vasopressin (VP).
- - It found that male offspring from stressed mothers displayed impaired social memory and increased anxiety, especially in the group where both mother and pup experienced stress (S:S), which correlated with a decrease in OT and VP-positive neurons in certain brain areas.
- - The results emphasize the significance of the prenatal period, suggesting that stress during pregnancy can lead to long-term behavioral issues in adult offspring, although alterations in the peptidergic systems only partially explain these deficits.