Publications by authors named "Pamela M L Leiva"

In this work, we calculated the body condition indices, K-Fulton and scaled mass index (SMI), of reproductive and non-reproductive adult females as an indication of stored energy. We considered 87 adult females captured from 2001 to 2018, both reproductive and non-reproductive. The body condition was calculated considering two scenarios: (a) only the weight of the female, and (b) the sum of the weight of the female and the average dry weight of her nest.

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Broad-snouted caiman (Caiman latirostris) products (meat, fat and oil) are currently beginning to be valued as a food of special interest due to its high content of n-3 fatty acids. Thus, the objective of this study was to characterize the fats of caiman fed with diets enriched with flaxseeds (Linus usitatissimun) rich in n-3 fatty acids, lignans and antioxidants. Caimans were fed six days a week with: a control diet (C), and a diet enriched with ground flaxseed = 90% C + 10% flaxseed ground (FS), during 30 (FS30) and 60 (FS60) days.

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Increasing polyunsaturated or long-chain fatty acids in meat for human consumption improves both nutritional quality and consumer perception. The increase could occur through the addition of rich sources of omega-3 fatty acids (such as flaxseed or flaxseed oil) to the animal diet. The aim of this study was to evaluate the effects of dietary supplement with two presentations of flax (crushed seeds or oil) on the change of FA content in two cuts of caiman meat (tail and neck).

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The broad snouted caiman is a crocodylian native to South America that is subject to extensive conservation management in both wild and farming environments. Although reproductive behaviors like egg laying and clutch care have been examined in this species, little else is known about their copulatory system. We examined the anatomy of male and female cloacal and genital tissues ex vivo to build hypotheses of their interactions during copulation and the effects of that interaction on insemination.

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Article Synopsis
  • Caiman latirostris fat is often discarded, but it offers a valuable source of essential fatty acids that can be utilized for human consumption.
  • The study focused on optimizing oil extraction from this fat and characterizing its nutritional and microbiological properties as a food supplement.
  • The extracted oil contains high-quality fatty acids (oleic acid 34%, linoleic acid 30%) and shows no harmful bacteria, remaining stable for up to 8 months under specific conditions.
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In recent decades, eggshells of eggs from large-bodied reptiles have been studied by many researchers, to describe the eggshell, to compare them to extinct lineages that once inhabited our planet and also to understand how the egg provides the embryo specific conditions during incubation. In previous studies we described and characterized normal and pathologic Caiman latirostris eggshells; we also evaluated how the eggshell changes during incubation. In a study relating temperature variation and eggshell structures of successful eggs, we observed empty structures not previously described that we termed "intracascaral space".

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Article Synopsis
  • Temperature plays a vital role in the embryonic development of reptiles, particularly for species like the Broad-snouted caiman which has temperature-dependent sex determination.
  • A study investigated the effects of constant (31°C, 32°C, 33°C) vs. fluctuating temperatures (31 ± 2, 32 ± 1, 32 ± 2°C) on various factors including sex ratio, hatching success, and hatchling characteristics.
  • Results showed that constant temperatures led to predictable sex ratios, while fluctuations affected hatchling size and success, indicating that both mean temperature and its variability significantly influence early development in caimans.
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Egg inundation often results in poor hatching success in crocodylians. However, how tolerant eggs are to submergence, and/or how eggshell ultrastructure may affect embryo survival when inundated, are not well understood. In this study, our objective was to determine if embryo survival in is affected by eggshell surface roughness, when eggs are submerged under water.

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