Publications by authors named "Pamela Flores"

Microorganisms' natural ability to live as organized multicellular communities - also known as biofilms - provides them with unique survival advantages. For instance, bacterial biofilms are protected against environmental stresses thanks to their extracellular matrix, which could contribute to persistent infections after treatment with antibiotics. Bacterial biofilms are also capable of strongly attaching to surfaces, where their metabolic by-products could lead to surface material degradation.

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The undesirable, yet inevitable, presence of bacterial biofilms in spacecraft poses a risk to the proper functioning of systems and to astronauts' health. To mitigate the risks that arise from them, it is important to understand biofilms' behavior in microgravity. As part of the Space Biofilms project, biofilms of Pseudomonas aeruginosa were grown in spaceflight over material surfaces.

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Article Synopsis
  • Fungi biofilms have been discovered on various spacecraft surfaces, and while their presence is problematic, it's challenging to prevent.
  • The study investigated how microgravity affects fungal biofilm formation by sending seven types of materials inoculated with fungal spores to the International Space Station for different incubation periods.
  • Results showed that microgravity generally didn't alter biofilm shape, biomass, or thickness, but it did impact formation rates based on material and incubation time, with nanograss exhibiting significantly less biofilm formation.
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Microbiologists are learning to appreciate the importance of "functional amyloids" that are produced by numerous bacterial species and have impacts beyond the microbial world. These structures are used by bacteria to link together, presumably to increase survival, protect against harsh conditions, and perhaps to influence cell-cell communication. Bacterial functional amyloids are also beginning to be appreciated in the context of host-pathogen interactions, where there is evidence that they can trigger the innate immune system and are recognized as non-self-molecular patterns.

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Research on the development of scientific reasoning has put the main focus on children's experimentation skills, in particular on the control-of-variables strategy. However, there are more scientific methods than just experimentation. Observation is defined as an independent scientific method that includes not only the description of what is observed, but also all phases of the scientific inquiry, such as questioning, hypothesizing, testing, and interpreting.

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