The effects of neurotensin on selected parameters of lipid metabolism in rats.

15 nM/kg b.m. of neurotensin (NT) caused a significant inhibition of LMA within 30 min of administration and this effect persisted up for to the 240 th minute of the experiment.

The influence of glimepiride on the binding kinetics of insulin with its skeletal muscle and liver receptors in rats with short term and prolonged hyperglycemia induced by streptozotocin.

Background: The aim of the study was to determine the influence of glimepiride on the binding kinetics of insulin with its skeletal muscle receptor in rats with transient and prolonged hyperglycemia induced by streptozotocin.

Material/methods: The studies were performed on healthy male Wistar rats with a body mass of 220+/-30 g, fed with LSM-type standard chow, and given water ad libitum. Transient or prolonged hyperglycemia was induced by intraperitoneal administration of streptozotocin.

The influence of glimepiride on the oxidative state of rats with streptozotocin-induced hyperglycemia.

Background: The aim of this work was to determine whether glimepiride, a derivate of sulphonylurea of the hypoglycemic effect, influences the level of prooxidative factors and antioxidative enzymes activity in the course of experimental streptozotocin hyperglycemia in rats.

Material/methods: The studies were performed on healthy male Wistar rats of body weight 200+/-30 g, fed with the standard laboratory diet and given water ad libitum. Animals were divided into 3 groups, each consisting of 8 rats.

The effect of brief food withdrawal on the level of free radicals and other parameters of oxidative status in the liver.

Background: The aim of our study was to determine the effect of brief starvation on the level of free radicals and other parameters of oxidative status in the liver.

Material/methods: The Wistar male rats used for the experiment were divided into two groups: Group I had free access to water and standard chow, while Group II was subjected to a 36-hour fast. The activity of antioxidant enzymes--catalase (CAT), glutathione peroxidase (GSH-Px) and Cu, Zn-superoxide dysmutase (CuZn-SOD)--was measured in the livers of both control and fasting animals.

The effect of bradykinin on the oxidative state of rats with acute hyperglycaemia.

Many clinical and experimental studies have established the beneficial effect of kinins in hypertension, heart failure and ischaemia-reperfusion syndrome, but little attention has been given to the role of kinins in hyperglycaemic conditions. The purpose of the present study was to determine the influence of bradykinin on the levels of glucose, insulin, malondialdehyde and hydrogen peroxide, as well as antioxidative enzyme activity in rats with streptozotocin (STZ)-induced acute hyperglycaemia. In STZ-induced hyperglycaemic rats the levels of glucose, hydrogen peroxide and malondialdehyde were increased by 256% (from 6.

[The level of erythropoietin in serum of patients with anemia during infective endocarditis].

The infective endocarditis is a septic syndrome caused by an infection in endocardium or in heart valves. The majority of patients with infective endocarditis develop normocytic anemia. The metabolic studies in septic shock syndromes documented an intensive proteolysis of muscles, visceral organs and blood proteins, and probably of erythropoietin as a glycoprotein as well.

[Neurotensin--structure, origin and biological function].

Neurotensin is a 13-amino acid hormonal peptide which was first isolated from bovine hypothalamus. It is present in the digestive tract as well as in the central nervous system. It has a variety of biological activities as a central neurotransmitter or neuromodulator, and a peripheral hormone.

[Lipid mobilizing activity in blood serum during the follicular and luteal phases of the menstrual cycle].

The lipid mobilising activity of woman's blood serum in the follicular and luteal phases has been investigated. This activity was determined according to the Buckle method concerning parametrial adipose tissue of female rats. It was found to enhance the lipid mobilising activity in the follicular phase of the menstrual cycle.

Effect of hypothermia on the insulin--receptor interaction in liver plasma membranes.

The aim of the study was to estimate the effect of hypothermia on (125J)-iodoinsulin binding to liver plasma membranes. Rat liver membranes were prepared from control, normothermic rats (Tr = 35.6 +/- 0.

[Effect of progesterone on lipolysis in blood and adipose tissue of the female rat].

The effect of progesterone on lipolysis in blood and in adipose tissue of female rats has been investigated. The hormone was administered to the studied animals subcutaneously during 20 days. The lipid mobilizing activity was determined in blood serum according to the four experimental protocols: test serum--test tissue, test serum--control tissue, control serum--test tissue, and control serum--control tissue.

[Effect of insulin on temperature and metabolic responses in rats during normothermia and hypothermia].

As shown in our previous study, hypothermia provokes a variety of hormonal changes including inhibition of insulin secretion and increase in blood serum glucagon level. According to Therminarias et al. the administration of exogenous insulin to dogs subjected to hypothermia causes a calorigenic effect by enhancing oxygen consumption and rising the intensity of shivering thermogenesis.

[Effect of tolbutamide on lipolytic processes in blood and fat tissue of rats maintained in normothermic and hypothermic conditions].

The effect of tolbutamide administered in vivo or added to the incubation medium on lipolysis in adipose tissue and in blood has been studied in rats. Tissue lipolysis was lowered by 118 to 156% in groups of rats receiving tolbutamide in vivo. The addition of tolbutamide directly to the incubation medium caused an increase in the lipid mobilizing activity, while the addition of insulin inhibited lipolysis in each case.

In vivo effect of 2-deoxy-D-glucose on adenine nucleotide levels in the liver and skeletal muscle of rats.

The present report indicates that 2-deoxy-D-glucose (2-DG) at a single dose causing reduction of Tre has no influence on liver and skeletal muscle content of ATP, ADP and AMP, the ATP/ADP ratio, energy charge potential (ECP) and total adenine nucleotides (TAN). After administration of 2-DG for 3) successive days, the level of ATP, ATP/ADP ratio, the values of ECP and TAN are decreased both in the liver and skeletal muscle. However, 72 hours after the last injection of 2-DG adenine nucleotide contents returned to the values observed in control group, indicating that the in vivo effect of this glucose analogue is fully reversible.

In vivo effect of 2-deoxy-D-glucose on glucose-6-phosphate dehydrogenase activity in the cytosol of liver, heart and skeletal muscle of rats.

2-deoxy-D-glucose (2-DG), the unmetabolizable analogue of glucose induces a series of metabolic, hormonal and behavioral responses, causing cellular glucoprivation. According to in vitro studies, 2-DG inhibits phosphofructokinase in cultured human cells. The present investigations deal with changes in the cytosolic glucose-6-phosphate dehydrogenase activity following in vivo 2-DG administration.

The effect of 4-aminopyrazolo (3,4-d) pyrimidine (4-APP) on some parameters of carbohydrate metabolism in the rat.

The study was designed to evaluate some parameters of carbohydrate metabolism in the rat as influenced by 4-APP, an adenine analogue. Adult female rats were injected with 1 mg 4-APP/100 g body weight/day for 3 days. 4-APP evoked a marked enlargement of the liver with lipid droplets accumulation in hepatocytes.

[Effect of estradiol on lipolytic processes in the blood and adipose tissue of female rats].

The effect of estradiol on lipolysis in blood and in adipose tissue of female rat has been investigated. The hormone was administered to the studied animals subcutaneously during 20 days. The lipid mobilizing activity was determined in blood serum according to the four different experimental protocols: test serum--test tissue, test serum--control tissue, control serum--test tissue, and control serum--control tissue.

Hyperthermia effect on lipolytic processes in rat blood and adipose tissue.

Rats anaesthetized with Brevinarcon were placed in a high-temperature chamber (air temperature 50 degrees C, relative humidity 50%) for induction of hyperthermia (rectal temperature 41.0 +/- +/- 0.5 degrees C).

Triiodothyronine effect on some parameters of carbohydrate metabolism in rat blood and liver. I. In vivo effect of triiodothyronine on liver glycogen content, blood metabolites and serum glycogenolytic activity.

Short (7 days) T3-treatment has no influence on the plasma glucose and FFA concentration and blood lactate level, as well as on the hepatic glycogen content in the rats. The rise in T3 levels, observed in our study, was accompanied by a fall in T4 concentrations, indicating suppression of the endogenous T4 production. On the other hand, glycogenolytic activity of rat serum in vitro is the highest in the system: control liver slices-serum of rats pretreated with T3 as compared to the system: control liver slices-control serum.

Adrenocortical function in 4-APP treated rats: a coupled stereological and biochemical study.

Adrenocortical function in 4-APP-induced (4-aminopyrazolo[3,4-d]pyrymidine) lipoprotein-deficient rats was studied in relation to quantitative morphologic changes in the gland. 4-APP treatment results in enlargement of the adrenal cortex and its zona fasciculata and reticularis cells. In enlarged livers, cholesterol and free fatty acid concentrations were similar to that of control rats, however a marked accumulation of triglycerides with a concomitant drop in hepatic delta 4-steroid hydrogenase activity was found.

[Energy expenditure and work exertion in clothing industry workers].

The maximum oxygen uptake (VO2max), oxygen consumption compared with VO2max values--percent of VO2max (strenuousness) and energy expenditure in clothing industry workers were investigated. Seventy five subjects took part in those investigations. The effective energy expenditure of weedle women was 2,3 KJ/480 min, store-workers--3,3 KJ/480 min, cutters 4,8 KJ/480 min, layers--5,1 KJ/480 min, and ironers from 3,7 KJ/480 min, to 4,7 KJ/480 min, respectively.

Effect of 2-deoxy-D-glucose on lipolytic processes in blood and adipose tissue of rat.

The effect of 2-deoxy-D-glucose on lipolytic processes in the blood and adipose tissue was studied. Rats treated with this antimetabolite showed a significant increase in serum glucose, FFA and glycerol level, as well as in the lipid mobilizing activity. On the other hand, the lipolytic activity of rat serum decreased when compared to control group.

Sex differences in adrenocortical structure and function. XIV. Heparin-releasable liver-lipase like activity in rat adrenals as affected by gonadectomy and testosterone or estradiol replacement.

Heparin-releasable liver-lipase like activity was studied in adrenals of intact, gonadectomized and gonadectomized-testosterone or estradiol replaced rats. Besides acid lipase, rat adrenals contain a neutral lipase activity. From both male and female adrenals about 70% of this activity is extractable by heparin containing medium.