PAP1 and PAP7 are required for association of plastid-encoded RNA polymerase with DNA.

Plastid-encoded RNA polymerase (PEP) is a bacterial-type multisubunit RNA polymerase responsible for the majority of transcription in chloroplasts. PEP consists of four core subunits, which are orthologs of their cyanobacterial counterparts. In Arabidopsis thaliana, PEP is expected to interact with 14 PEP-associated proteins (PAPs), which serve as peripheral subunits of the RNA polymerase.

Membrane association of active genes organizes the chloroplast nucleoid structure.

DNA is organized into chromatin-like structures that support the maintenance and regulation of genomes. A unique and poorly understood form of DNA organization exists in chloroplasts, which are organelles of endosymbiotic origin responsible for photosynthesis. Chloroplast genomes, together with associated proteins, form membrane-less structures known as nucleoids.

N -Methyladenosine modification of mRNA contributes to the transition from 2D to 3D growth in the moss Physcomitrium patens.

Plants colonized the land approximately 470 million years ago, coinciding with the development of apical cells that divide in three planes. The molecular mechanisms that underly the development of the 3D growth pattern are poorly understood, mainly because 3D growth in seed plants starts during embryo development. In contrast, the transition from 2D to 3D growth in the moss Physcomitrium patens has been widely studied, and it involves a large turnover of the transcriptome to allow the establishment of stage-specific transcripts that facilitate this developmental transition.

High-resolution map of plastid-encoded RNA polymerase binding patterns demonstrates a major role of transcription in chloroplast gene expression.

Plastids contain their own genomes, which are transcribed by two types of RNA polymerases. One of those enzymes is a bacterial-type, multi-subunit polymerase encoded by the plastid genome. The plastid-encoded RNA polymerase (PEP) is required for efficient expression of genes encoding proteins involved in photosynthesis.

Preferences for Renal Cell Carcinoma Pharmacological Treatment: A Discrete Choice Experiment in Patients and Oncologists.

Introduction: The purpose of this investigation was to explore patients' and oncologists' preferences for the characteristics of a pharmacological regimen for patients with advanced renal cell carcinoma (aRCC).

Material And Methods: Cross-sectional observational study based on a discrete choice experiment (DCE) conducted in Spain. A literature review, a focus group with oncologists and interviews with patients informed the DCE design.

Biomolecular insights into North African-related ancestry, mobility and diet in eleventh-century Al-Andalus.

Historical records document medieval immigration from North Africa to Iberia to create Islamic al-Andalus. Here, we present a low-coverage genome of an eleventh century CE man buried in an Islamic necropolis in Segorbe, near Valencia, Spain. Uniparental lineages indicate North African ancestry, but at the autosomal level he displays a mosaic of North African and European-like ancestries, distinct from any present-day population.

The canonical RdDM pathway mediates the control of seed germination timing under salinity.

Plants respond to adverse environmental cues by adjusting a wide variety of processes through highly regulated mechanisms to maintain plant homeostasis for survival. As a result of the sessile nature of plants, their response, adjustment and adaptation to the changing environment is intimately coordinated with their developmental programs through the crosstalk of regulatory networks. Germination is a critical process in the plant life cycle, and thus plants have evolved various strategies to control the timing of germination according to their local environment.

In the immuno-oncology era, is anti-PD-1 or anti-PD-L1 immunotherapy modifying the sensitivity to conventional cancer therapies?

Introduction: The advent of anti-programmed death receptor-1/ligand-1 antibodies (anti-PD(L)1) is profoundly changing the therapeutic strategy of oncology. As anti-PD(L)1 modulate tumour microenvironment, it might impact sensitivity to conventional cancer therapy (CCT). Therefore, we explored whether sensitivity to CCT was different before and after anti-PD(L)1 therapy.

Insights into the function of the phasiRNA-triggering miR1514 in response to stress in legumes.

We recently described the activity of miR1514a in response to water deficit in Phaseolus vulgaris. Pvu-miR1514a targets a NAC transcription factor mRNA for cleavage and subsequently triggers NAC-derived phasiRNA formation. Here we show that accumulation and activity of miR1514a are also conserved in the model legume Medicago truncatula.

Frequency and characteristics of familial melanoma in Spain: the FAM-GEM-1 Study.

Introduction: Familial history of melanoma is a well-known risk factor for the disease, and 7% melanoma patients were reported to have a family history of melanoma. Data relating to the frequency and clinical and pathological characteristics of both familial and non-familial melanoma in Spain have been published, but these only include patients from specific areas of Spain and do not represent the data for the whole of Spain.

Patients And Methods: An observational study conducted by the Spanish Group of Melanoma (GEM) analyzed the family history of patients diagnosed with melanoma between 2011 and 2013 in the dermatology and oncology departments.

[Experimental model of recurrent acute otitis media: morphological study of the cochlear damage using scanning electron microscopy].

We designed an experimental model of acute otitis media to study its effects in the hair cells of the organ of Corti. Thirty Sprague-Dawley rats were used, and divided into three groups. The rats from the first group were exposed to one episode of acute otitis media, the second group to two episodes and the third group were induced with three episodes of acute otitis media.

[Otosclerosis surgery with CO2 laser].

Since Shea described the stapedectorny, many physicians have developed modifications of the technique, prosthesis or instrumentation. We have adopted one of the most recent advances to the treatment of otosclerosis, CO2 laser. Interventions were carried out in 20 consecutive ears using CO2 laser.

[Fungal sinusitis. Report of four cases].

Fungal sinusitis has a nonspecific clinical presentation, is refractory to standard medical treatment and may produce expansion and erosion of the sinus wall. Various factors have been implicated in the development of fungal sinusitis: anatomical factors in the osteomeatal complex, tissular hypoxia, traumatic factors, massive exposure to fungal spores, allergy and immunosuppression. Our study of four cases diagnosed in the department included the clinical, pathological, and radiological features of fungal sinusitis.

[Peritonsillar abscess: treatment guidelines].

A prospective study was carried out on 25 patients showing symptoms suggestive of peritonsillar abscess. Twelve patients were treated with i.v.

[Inverted papillomas of the nasal sinuses].

After reviewing the Arnau de Vilanova Hospital's records, a total of ten cases of inverted papillomas were found. Nasal obstruction was present in 100% of the cases. External surgery was performed in 70% of these patients, finding that in 50% of them, the real size of the tumor was smaller than that show in the radiological study.

Bacterial flora in the external ear canal and in induced cholesteatoma in the Mongolian gerbil.

We report on the bacterial flora of the external ear canal and of induced aural cholesteatoma in 14 Mongolian gerbils (Meriones unguiculatus). In the control, nonmanipulated external ear canal, gram-positive and gram-negative organisms were isolated. In the contents of the cholesteatoma sac, only monomicrobial, gram-negative isolates were found; in each animal they were different from the isolates from the nonmanipulated external ear canal.

[Lymphoepithelioma of the larynx].

Non-nasopharyngeal Lymphoepithelioma is a tumor of undifferentiated non-keratinizing cells enclosed in an lymphocytic stroma. The lymphoid tissue does not participate in the tumor process. Its tendency is to precociously spreading in the lymph nodes and viscera, but shows a good radiotherapeutic response.

[Cervical cystic hygroma].

Hygroma being a rare overgrowth the AA. of the paper consider interesting the report of a case sitting in the neck, diagnosed and treated at Arnau de Vilanova Hospital. Memento concerning this entity.

[Review of ORL manifestations in AIDS. Case series at the Arnau de Vilanova Hospital, Lleida].

The majority of patients suffering AIDS-syndrome present with ENT aspects, being the most prevalent lateral adenopathy of the neck firstly, followed by thrush, pharyngo-esophageal candidiasis and herpes simplex. Differences regarding the distribution in percentage in risk groups are signaled by the AA. between their own statistics and those of the americans writers.

[Recurrent polychondritis].

The interest of the relapsing polychondritis is specially due to its otolaryngological implications. The case reported evolved favourably and responded fairish to the corticoidtherapy. Nevertheless an assidous follow-up will be wise in order to stablish the pronostic in view of the outbreacks of the illness.

[Tuberculous otitis media].

Report of a case of middle ear tuberculosis in an elderly woman with a chronic discharging ear withstanding the common treatment. The diagnosis could be made through examination of the pieces taken at surgery (tympanoplasty). The A.

[Otic cholesteatoma. Some comments].

Comments of the AA regarding the diagnosis and management of cholesteatoma and description of their current surgical procedure are presented.